慢性乙肝病毒感染者外周血树突状细胞亚群与血清HBVDNA的关系研究

Objective To investigate the influence of serum HBVDNA levels on the characterizathion of circulating dendritic cell subsets in patients with chronic hepatitis B infection.Methods The plasmacytoid DC (DC2, Lineage-HLA-DR+CD123+) and non-IFN-alpha producingmyeloid DC (DC1, Lineage-HLA-DR+CD11c+) in fresh peripheral blood from chronic hepatitis B infection without any anti-viral treatment and healthy donors were enumerated by using four-color flow cytometry.The serological markers of hepatitis B virus were evaluated by solid phase radioimmunoassay (RIA) .Results Percentage of PDC in patientswith chronic hepatitis B was numerically decreased and subsequently Produce less than that of nomal controls, but without statistical difference.Further, serum HBeAg positive patients with chronic hepatitis B exhibited a significant increase in percentage of circulating PDC, compared with HBeAg negative patients and health donors (P<0.01;P<0.05, respectively) .And there is no significant difference of MDC in peripheral blood between chronic hepatitis B patients and nomal controls or among three groups above (P=0.194) .Conclusion The results suggested that the HBV infection may cause patient's immunity function change and lead to the immune tolerance or apoptosis;The percentage of peripheral PDC is associated with the HBeAg seroconversion and may plays a role on inhibition of HBV replication in patients with chronic hepatitis B.