The Changes in substance P in jejunum of fatty liver

Objective To observe the expression of substance P (SP) in the mesenteric plexus of jejunum in a fatty liver rat model, therefore, investigate their possible role in the pathogenesis of gastrointestinal dysfunction in fatty liver.Methods Thirty SD rats were selected and randomly divided into the normal control group (n=10) and the fatty liver model group (n=20) .Fatty liver model were fed with high-fat diet.The hepatic tissue was sliced for pathological observation.The blue dextran-2000 was used to label gastrointestinal tract for gastric lavage to quantify the enteric transmission rate after three months.The tissue specimens were obtained from the control and the fatty liver model groups.The expression of SP-positive fibers was analyzed by immuonhistochemistry.The results were examined by means of color image analyzer.Results Compared with that of the controls, the small intestinal transmission rate of fatty liver rats was significantly reduced (P < 0.05) , the expressions of SP decreased (P < 0.05) .Conclusion The decrease of substance P in the mesenteric plexus of jejunum by the fatty liver rat model has a relationship with the decrease of movement of intestine.