Objective To investigate the effect of exogenous recombinant mouse insulin-like growth factor binding protein related protein 1 (rmIGFBPrP1) on both liver and kidney tissue in mouse, and explore the mechanism of rmIGFBPrP1 on fibrosis.Methods A total of 26 clean male wild-type C57BL/6 mice were randomly divided into normal control group, sham-operated group and model group.The rmIGFBPrP1 which was released by capsule osmotic pump was used to make the model.HE staining and Sirius red staining were observed to investigate the pathological changes and deposition of collagen fibers in liver and kidney of mice.The expression and distribution of IGFBPrP1, Smad3, p-Smad2/3, III collagen (Collagen III) of liver and kidney tissues were detected by immunohistochemistry.Hepatocyte apoptosis was detected by DUTP nick end labeling (TUNEL) .Results Comparing with the control group and sham-operated group, the content of collagen fiber and the expression of IGFBPrP1, Smad3, p-Smad2/3, Collagen III increased (P<0.01) in the liver of model group, While only the expression of IGFBPrP1 in the kidney of model group increased than that in the control group and sham-operated group (P<0.01) , there is no difference of collagen fibers content and other indicators expression in these three groups (P>0.05) .TUNEL showed the increase in ratio of apoptotic liver cells in model group than that in normal group and sham-operated group (P<0.01) .Conclusion Exogenous IGFBPrP1 can promote hepatocyte apoptosis and liver fibrosis, while have little effect on the mice kidney.IGFBPrP1 may selectively induce organs and organization fibrosis in mice.
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