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CN 22-1108/R
Volume 40 Issue 1
Jan.  2024
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Article Navigation >  Journal of Clinical Hepatology  > 2024  >  40(1): 70-75

Changes in the total volume and contour density of oval cells in hepatic lobules of rats with carbon tetrachloride-induced hepatic fibrosis： A stereological study

DOI: 10.12449/JCH240113
  • 1.

    Department of Infectious Diseases，Affiliated Hospital of North Sichuan Medical College，Nanchong，Sichuan 637000，China

  • 2.

    Department of Clinical Medicine，North Sichuan Medical College，Nanchong，Sichuan 637000，China

  • 3.

    Institute of Basic Medicine and Forensic Medicine，North Sichuan Medical College，Nanchong，Sichuan 637000，China

Research funding:

North Sichuan Medical College 2021 School-Level Scientific Research Development Plan Project (CBY21-QA14);

Nanchong Special Project for Municipal and University Science and Technology Strategic Cooperation in 2020 (20SXQT0304)

More Information
  • Corresponding author: PENG Bin， 340255492@qq.com （ORCID： 0000-0002-6947-2570）
  • Received Date: 2023-03-22
  • Accepted Date: 2023-06-05
  • Published Date: 2024-01-23
    Abstract
  •   Objective  To quantitatively investigate the changes in the total volume and contour density of hepatic oval cells （HOC） in hepatic lobules of rats with carbon tetrachloride （CCl4）-induced hepatic fibrosis.  Methods  A total of 11 healthy male Sprague-Dawley rats were randomly divided into control group with 5 rats and hepatic fibrosis group with 6 rats， and CCl4 and olive oil suspension were injected subcutaneously twice a week， 3 mL/kg each time. After five weeks of hepatic fibrosis modeling， five liver tissue blocks with a size of about 1 mm3 were randomly selected from the liver of each rat to prepare one Epon812 epoxy resin-embedded ultrathin section， and the stereological method and transmission electron microscopy were used for the quantitative analysis of the total volume and contour density of HOC in the hepatic lobules of rats. In addition， four liver tissue blocks with a thickness of 2 mm were randomly selected from the remaining liver of each rat to prepare two paraffin-embedded Masson staining sections， and the degree of liver fibrosis in each rat was qualitatively evaluated according to the Metavir staging criteria for liver fibrosis. The independent-samples t test was used for comparison of continuous data between groups.  Results  The quantitative stereological analysis showed that the total volume of HOC in hepatic lobules was 15.40±7.63 mm3 in the control group and 146.80±114.00 mm3 in the liver fibrosis group， and compared with the control group， the total volume of HOC in hepatic lobules of rats in the liver fibrosis group was significantly increased by 8.53 times （t=-2.551， P=0.031）； the contour density of HOC in hepatic lobules was 56.20±40.40 in the control group and 566.50±317.00 in the liver fibrosis group， and compared with the control group， the contour density of HOC in hepatic lobules of rats in the liver fibrosis group was significantly increased by 9.08 times （t=-3.539， P=0.006）. Qualitative observation showed that liver fibrosis stage of rats reached stage Ⅱ-Ⅲ according to the Metavir scoring criteria， and massive proliferation of HOC was observed around the proliferation site of hepatic stellate cells in the perisinusoidal space of rats.  Conclusion  CCl4 induces significant proliferation of HOC in hepatic lobules of rats with liver fibrosis.

     

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