Objective To investigate the role and action mechanism of Ganshuang granules in the protection against CCl4- induced chronic liver injury in mice via autophagy,and to provide a basis for its clinical application. Methods We established a chronic liver injury model in mice by intraperitoneal injection of CCl4,and a cell model of liver damage in cell line HL- 7702 induced by CCl4 in vitro. The animals were divided into three groups,Ganshuang granule intervention group,normal control group,and CCl4 group without receiving Ganshuang granules. In addition,we exposed the cells to 3- methyladenine( 3- MA),an autophagy inhibitor,and observed the effects of Ganshuang granules on cell apoptosis. Liver tissue injury was evaluated by HE staining; serum alanine aminotransferase( ALT) and aspartate aminotransferase( AST) levels were determined by biochemical assays. Autophagy was assessed by immunofluorescence staining and Western blotting. Liver apoptosis was analyzed by flow cytometry and Annexin V / PI double staining. Comparisons between groups were performed using analysis of variance. Results HE staining showed that liver tissue injury was significantly milder in the Ganshuang granule intervention group than in the CCl4 group. Serum ALT and AST levels were significantly differences between Ganshuang granule intervention group,normal control group,and CCl4group( F = 15. 76、13. 35,P < 0. 05). Both in vivo and in vitro tests showed that autophagy increased significantly in cells treated with Ganshuang granules than in cells exposed to CCl4 alone,while apoptosis was significantly reduced in the former.The administration of 3- MA weakened the protective effect of Ganshuang granules and increased apoptosis. Flow cytometry showed that apoptosises were significiantly differences between five groups( F = 25. 637,P < 0. 01). Conclusion Ganshuang granules have protective effects against chronic liver injury by inhibiting apoptosis,possibly through enhanced autophagy.
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