Objective To investigate the identification and treatment of variation of extrahepatic bile duct in laparoscopic cholecystectomy( LC),and to reduce the occurrence of bile duct injury. Methods This study included 60 patients who received LC in the People's Hospital of Caidian District in Wuhan and had structural variation of extrahepatic bile duct found during the operation from January 2012 to January2014. The clinical data were retrospectively analyzed,and the intraoperative and postoperative conditions were summarized. Results During operation,cystic duct variation was found in 32 cases,abnormal position of the point where the cystic duct joins the extrahepatic bile duct in 20 cases,the cystic duct and the common hepatic duct having the common wall before joining the common bile duct in 2 cases,aberrant bile duct in the gallbladder bed in 2 cases,and accessory hepatic duct in 4 cases. Fifty- one patients( 85%) successfully underwent LC; 9 patients( 15%) were converted to open surgery. All patients finished surgery successfully. There were 2 cases of postoperative complications; one patient developed residual stones in the bile duct,and bile leakage occurred in the other patient at one week after LC,who recovered after reoperation. All patients were cured and discharged,without severe complications such as intraperitoneal hemorrhage,infection,and intestinal injury. Conclusion Identifying the structural variation of extrahepatic bile duct,dissecting the Calot's triangle meticulously,and determining the type of variation of extrahepatic bile duct play important roles in LC and significantly reduce the incidence of bile duct injury.