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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 3
Mar.  2016
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Article Contents

Antifibrotic mechanism of Fuzheng Huayu prescription by regulation of the differential expression of microRNAs in mouse liver

DOI: 10.3969/j.issn.1001-5256.2016.03.022
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  • Published Date: 2016-03-20
  • Objective To identify microRNAs( miRNAs) regulated by Fuzheng Huayu prescription( FZHY) and analyze their biological functions,and to partially reveal the antifibrotic mechanism of FZHY in liver fibrosis. Methods The mouse model of liver fibrosis was developed by subcutaneous injection of CCl4. The mice were divided into normal group,model group,and FZHY group. The mice received FZHY once a day at 3 days before model establishment,and the treatment lasted for 8 weeks. Collagen deposition in liver tissue was evaluated by Sirius Red staining and determination of hydroxyproline( Hyp) content. The expression profile of miRNAs in mouse liver was determined by miRNA microarray. According to the expression profile,miRNAs regulated by FZHY were identified by looking for miRNAs showing the same trends in the normal group and the FZHY group compared with the model group. The results were confirmed by quantitative real- time PCR. The miRNA targets were predicted using Target Scan program and PITA database. The DAVID database was used to analyze and identify the substantial functions and signaling pathways of those miRNA targets. Comparison between multiple groups was made by analysis of variance. Results FZHY substantially reduced Hyp content and inhibited collagen deposition in the fibrotic liver tissue. The miRNA microarray identified mmu- miR- 322,mmu- miR- 342- 3p,and mmu- miR- 296- 5p as miRNAs regulated by FZHY. According to the analysis of signaling pathway,the three miRNAs might regulate 32 signaling pathways,including chemokine signaling pathway,focal adhesion,MAPK signaling pathway,regulation of actin cytoskeleton,gap junction,ECM- receptor interaction,Wnt signaling pathway,and Jak- STAT signaling pathway,which were closely related to liver fibrosis; the functional enrichment analysis predicted 32 substantial functions of the three miRNAs,including GTPase regulator,cell junction,regulation of apoptosis,regulation of Ras signal transduction,and small GTPase regulator,which were closely related to liver fibrosis. Conclusion The antifibrotic effect of FZHY in the liver might be achieved by down- regulation of the expression of miR- 322,miR- 342- 3p,and miR- 296- 5p,which regulates their specific functions,such as MAPK signaling pathway,Wnt signaling pathway,regulation of apoptosis,and regulation of Ras signal transduction. The conclusion deserves further investigation.

     

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