Objective To investigate the influence of specific down-regulation of LKB1 expression on invasion and migration of the hepatoma cell line SK-hep-1.Methods Quantitative real-time PCR (RT-PCR) and Western blot were used to measure the expression of LKB1 in the human hepatoma cell line SK-hep-1 and normal hepatocytes (HL7702) .LKB1 specific double-strand small interfering RNA was designed, synthesized, and then transfected into the human hepatoma cell line SK-hep-1 with high expression of LKB1 to silence the expression of LKB1 gene.A negative control (NC) group was established.RT-PCR and Western blot were used to measure the changes in the mRNA and protein expression of LKB1.Cell Counting Kit-8 was used to measure the proliferative capacity of hepatoma cells.In vitro Transwell chamber invasion and migration assays were used to observe the influence of LKB1 gene silencing on invasion and migration of the human hepatoma cell line SK-hep-1.The t-test was used for comparison of continuous data between two groups;a one-way analysis of variance was used for comparison between multiple groups, and the least significant difference t-test was used for further comparison between any two groups.Results Compared with normal human hepatocytes (HL7702) , SK-hep-1 cells had significantly higher mRNA and protein expression of LKB1 (both P<0.05) .Compared with the NC group, the siLKB1-1 treatment group had significant reductions in the mRNA and protein expression of LKB1 in SK-hep-1 cells (both P<0.05) .Compared with the NC group, the siLKB1-1 treatment group had a significantly higher cell proliferation rate and significant increases in invasion and migration (1.393 ±0.022 vs 1.1284±0.032, t=15.313, P<0.001;147±19 vs 83±21, t=14.879, P<0.001;113±13 vs 75±17, t=8.791, P<0.001) .Conclusion LKB1 may be involved in inhibiting the proliferation, invasion, and migration of hepatoma cells and can affect the prognosis of patients with liver cancer.
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