Effect of lobaplatin on proliferation and apoptosis of hepatocellular carcinoma HepG2 cells and related mechanism of action

Objective To investigate the effect of lobaplatin on the proliferation and apoptosis of hepatocellular carcinoma (HCC) HepG2 cells in vitro and related mechanism of action.Methods HepG2 cells were divided into lobaplatin group (proliferation assay:5 μmol/L, 10 μmol/L, and 15 μmol/L; apoptosis assay:10 μmol/L; invasion assay:4 μg/ml) and control group (no drug was added) .CCK-8 assay and flow cytometry were used to measure the proliferation and apoptosis of HepG2 cells.Western Blot was used to measure the change in the expression of NF-κB, Bcl-2, Bax, Bid, Puma, and caspase-3 proteins involved in proliferation and apoptosis.A two-way analysis of variance was used for proliferation assay, the chi-square test was used for apoptosis assay, and the t-test was used for invasion assay.Results After intervention for 24, 48, and 72 hours, there was a significant difference in proliferation inhibition rate between the two groups (F = 273.5, P < 0.01) ; there was also a significant difference between different concentration groups at the same time point (F =1857, P < 0.01) , and all the concentration groups showed a significant change over time (F = 1365, P < 0.01) .As for apoptosis, there was a significant difference in apoptosis rate between the two groups (χ2= 1821, P < 0.001) .As for invasion ability, compared with the control group, the lobaplatin group had a significantly lower number of cells passing through the Transwell chamber (21.30 ± 2.74 vs 45.00± 4.26, t = 11.89, P < 0.001) .As for proteins, the lobaplatin group had a significant reduction in the expression of Bcl-2 and significant increases in the expression of NF-κB, Bax, Puma, and caspase-3.Conclusion Lobaplatin exerts a certain effect on the proliferation and apoptosis of HCC HepG2 cells by affecting the proteins involved in proliferation and apoptosis, but further studies are needed due to complex signaling pathways in cells.