Effect of Pien Tze Huang on bile excretion and its analgesic effect in experimental animals

Objective To investigate the effect of Pien Tze Huang on bile excretion and its analgesic effect in experimental animals. Meth-ods A total of 30 guinea pigs were randomly divided into Pien Tze Huang group, ursodeoxycholic acid group, and control group. Accordingto body mass, the three groups were treated with Pien Tze Huang ( 140 mg/kg) , ursodeoxycholic acid ( 22. 5 mg/kg) , and normal saline, respectively, by gavage for 4 consecutive days. Then bile drainage was performed to measure the volume of bile secretion and the change inbile composition. Related liver function parameters were also measured. A total of 30 mice were randomly divided into Pien Tze Huang group ( 360 mg/kg) , ursodeoxycholic acid group ( 120 mg/kg) , and control group. At one hour after the last administration, the mice were givenintraperitoneal injection of 0. 6% glacial acetic acid ( 0. 15 ml/10 g) , and the writhing test was performed to observe the analgesic effect ofPien Tze Huang. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least signifi-cant difference t-test was used for further comparison between two groups. Results Compared with the control group, the Pien Tze Huanggroup and the ursodeoxycholic acid group had a significant increase in the volume of bile secretion ( P = 0. 039 and 0. 009) . There were nosignificant differences between the three groups in cholesterol, bilirubin, total bile acid, and phospholipid in bile ( all P > 0. 05) and liverfunction parameters ( alanine aminotransferase, aspartate aminotransferase, gamma-glutamyl transpeptidase, total bilirubin, direct biliru-bin, indirect bilirubin, and total bile acid) ( all P > 0. 05) . Compared with the control group, the Pien Tze Huang group had a significantreduction in the number of writhing times ( P < 0. 001) , suggesting that Pien Tze Huang had a marked analgesic effect. Conclusion Ani-mal experiments show that Pien Tze Huang has marked choleretic and analgesic effects, which provides theoretical and data support for theclinical application of Pien Tze Huang.