|
[1]REVILL PA, CHISARI FV, BLOCK JM, et al.A global scientific strategy to cure hepatitis B[J].Lancet Gastroenterol Hepatol, 2019.[Epub ahead of print]
|
|
[2]YAN H, ZHONG G, XU G, et al.Sodium taurocholate cotransporting polypeptide is a functional receptor for human hepatitis B and D virus[J].Elife, 2012, 1:e00049.
|
|
[3]GUO JT, GUO H.Metabolism and function of hepatitis B virus cccDNA:Implications for the development of cccDNA-targeting antiviral therapeutics[J].Antiviral Res, 2015, 122:91-100.
|
|
[4]SEEGER C, MASON WS.Hepatitis B virus biology[J].Microbiol Mol Biol Rev, 2000, 64 (1) :51-68.
|
|
[5]PERRILLO R.Benefits and risks of interferon therapy for hepatitis B[J].Hepatology, 2009, 49 (5 Suppl) :s103-s111.
|
|
[6]DURANTEL D, ZOULIM F.New antiviral targets for innovative treatment concepts for hepatitis B virus and hepatitis delta virus[J].J Hepatol, 2016, 64 (1 Suppl) :s117-s131.
|
|
[7]CHEN Y, SZE J, HE ML.HBV cccDNA in patients'sera as an indicator for HBV reactivation and an early signal of liver damage[J].World J Gastroenterol, 2004, 10 (1) :82-85.
|
|
[8]YU XH, WANG Y, YANG TT.Research advances in clinical application of hepatitis B virus covalently closed circular DNA[J].Chin J Nosocomiol, 2013, 23 (17) :4338-4340. (in Chinese) 余晓红, 王媛, 杨廷桐.乙型肝炎病毒cccDNA临床应用研究新进展[J].中华医院感染学杂志, 2013, 23 (17) :4338-4340.
|
|
[9]TUTTLEMAN JS, POURCEL C, SUMMERS J.Formation of the pool of covalently closed circular viral DNA in hepadnavirusinfected cells[J].Cell, 1986, 47 (3) :451-460.
|
|
[10]ZHANG YY, ZHANG B, THEELE D, et al.Single-cell analysis of covalently closed circular DNA copy numbers in a hepadnavirus-infected liver[J].Proc Natl Acad Sci U S A, 2003, 100 (21) :12372-12377.
|
|
[11]DANDRI M, BURDA MR, WILL H, et al.Increased hepatocyte turnover and inhibition of woodchuck hepatitis B virus replication by adefovir in vitro do not lead to reduction of the closed circular DNA[J].Hepatology, 2000, 32 (1) :139-146.
|
|
[12] WERLE-LAPOSTOLLE B, BOWDEN S, LOCARNINI S, et al.Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy[J].Gastroenterology, 2004, 126 (7) :1750-1758.
|
|
[13]NASSAL M.HBV cccDNA:Viral persistence reservoir and key obstacle for a cure of chronic hepatitis B[J].Gut, 2015, 64 (12) :1972-1984.
|
|
[14]ITZKOVITZ S, van OUDENAARDEN A.Validating transcripts with probes and imaging technology[J].Nat Methods, 2011, 8 (4Suppl) :s12-s19.
|
|
[15]GALL JG, PARDUE ML.Formation and detection of RNA-DNAhybrid molecules in cytological preparations[J].Proc Natl Acad Sci U S A, 1969, 63 (2) :378-383.
|
|
[16]JOHN HA, BIRNSTIEL ML, JONES KW.RNA-DNA hybrids at the cytological level[J].Nature, 1969, 223 (5206) :582-587.
|
|
[17]BAUMAN JG, WIEGANT J, BORST P, et al.A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA[J].Exp Cell Res, 1980, 128 (2) :485-490.
|
|
[18]LANGER PR, WALDROP AA, WARD DC.Enzymatic synthesis of biotin-labeled polynucleotides:Novel nucleic acid affinity probes[J].Proc Natl Acad Sci U S A, 1981, 78 (11) :6633-6637.
|
|
[19]NAOUMOV NV, ALEXANDER GJ, EDDLESTON AL, et al.In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens:Method for detecting human and viral DNA using biotinylated probes[J].J Clin Pathol, 1988, 41 (7) :793-798.
|
|
[20]HEILES HB, GENERSCH E, KESSLER C, et al.In situ hybridization with digoxigenin-labeled DNA of human papillomaviruses (HPV 16/18) in He La and Si Ha cells[J].Biotechniques, 1988, 6 (10) :978-981.
|
|
[21]HAASE AT, RETZEL EF, STASKUS KA.Amplification and detection of lentiviral DNA inside cells[J].Proc Natl Acad Sci US A, 1990, 87 (13) :4971-4975.
|
|
[22]KERN D, COLLINS M, FULTZ T, et al.An enhanced-sensitivity branched-DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma[J].J Clin Microbiol, 1996, 34 (12) :3196-3202.
|
|
[23]HENDRICKS DA, STOWE BJ, HOO BS, et al.Quantitation of HBV DNA in human serum using a branched DNA (bDNA) signal amplification assay[J].Am J Clin Pathol, 1995, 104 (5) :537-546.
|
|
[24]DETMER J, LAGIER R, FLYNN J, et al.Accurate quantification of hepatitis C virus (HCV) RNA from all HCV genotypes by using branched-DNA technology[J].J Clin Microbiol, 1996, 34 (4) :901-907.
|
|
[25]PLAYER AN, SHEN LP, KENNY D, et al.Single-copy gene detection using branched DNA (bDNA) in situ hybridization[J].J Histochem Cytochem, 2001, 49 (5) :603-612.
|
|
[26]REN JY, CHEN ML, TIAN GS, et al.Application of in situ hybridization in research on hepatitis B[J].Foreign Med Sci:Epidemiol Loimol, 1988, 6:245-248. (in Chinese) 任进余, 陈梅龄, 田庚善, 等.原位杂交技术在乙型肝炎研究中的应用[J].国外医学.流行病学传染病学分册, 1988, 6:245-248.
|
|
[27]GOWANS EJ, BURRELL CJ, JILBERT AR, et al.Detection of hepatitis B virus DNA sequences in infected hepatocytes by in situ cytohybridisation[J].J Med Virol, 1981, 8 (1) :67-78.
|
|
[28]NURIYA H, INOUE K, TANAKA T, et al.Detection of hepatitis B and C viruses in almost all hepatocytes by modified PCR-based in situ hybridization[J].J Clin Microbiol, 2010, 48 (11) :3843-3851.
|
|
[29]KENNY D, SHEN LP, KOLBERG JA.Detection of viral infection and gene expression in clinical tissue specimens using branched DNA (bDNA) in situ hybridization[J].J Histochem Cytochem, 2002, 50 (9) :1219-1227.
|
|
[30]CALABRESE D, WIELAND SF.Highly sensitive detection of HBVRNA in liver tissue by in situ hybridization[J].Methods Mol Biol, 2017, 1540:119-134.
|
|
[31]YEH CT, CHIU HT, CHU CM, et al.G1 phase dependent nuclear localization of relaxed-circular hepatitis B virus DNA and aphidicolin-induced accumulation of covalently closed circular DNA[J].J Med Virol, 1998, 55 (1) :42-50.
|
|
[32]ZHONG Y, HU S, XU C, et al.A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR[J].BMC Infect Dis, 2014, 14:608.
|
|
[33]ZHANG X, LU W, ZHENG Y, et al.In situ analysis of intrahepatic virological events in chronic hepatitis B virus infection[J].J Clin Invest, 2016, 126 (3) :1079-1092.
|
|
[34]LI M, SOHN JA, SEEGER C.Distribution of hepatitis B virus nuclear DNA[J].J Virol, 2018, 92 (1) :e01391-17.
|
|
[35]ZHANG X, YUE L, ZHANG Z, et al.Establishment of a fluorescent in situ hybridization assay for imaging hepatitis B virus nucleic acids in cell culture models[J].Emerg Microbes Infect, 2017, 6 (11) :e98.
|
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