中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 35 Issue 6
Jun.  2019
Turn off MathJax
Article Contents

Recent advances of quantitative detection methods for HBV cccDNA

DOI: 10.3969/j.issn.1001-5256.2019.06.006
  • Received Date: 2019-05-09
  • Published Date: 2019-06-20
  • Elimination of HBV cccDNA from hepatocytes infected with chronic HBV virus is considered to be the key to eradicating HBV.Monitoring HBV cccDNA before, during, and after viral treatment is essential for routine treatment of patients with chronic hepatitis B. With the introduction of new anti-HBV treatment technologies and new drugs targeting HBV cccDNA, Accurate and sensitive HBV cccDNA assays are urgently needed to evaluate efficacy. In recent years, HBV cccDNA detection methods have achieved gratifying results in both traditional PCR methods and digital PCR methods popular in recent years. In this paper, the advances in HBV cccDNA quantitative detection by qPCR, Magnetic bead capture hybridization, rolling circle amplification combined with in situ PCR, digital PCR and digital PCR assay in single cells were reviewed.

     

  • loading
  • [1]RAZAVI-SHEARER D, GAMKRELIDZE I, NGUYEN MH, et al.Global prevalence, treatment, and prevention of hepatitis B virus infection in 2016:A modelling study[J].Lancet Gastroenterol Hepatol, 2018, 3 (6) :383-403.
    [2]CHEN J, WU M, LIU K, et al.New insights into hepatitis B virus biology and implications for novel antiviral strategies[J].Nat Sci Rev, 2015, 2 (4) :296-313.
    [3]SEEGER C, MASON WS.Molecular biology of hepatitis B virus infection[J].Virology, 2015, 8 (12) :672-686.
    [4]LI XL, ZHAO JH, YUAN Q, et al.Detection of HBV covalently closed circular DNA[J].Viruses, 2017, 9 (3) :139-158.
    [5]CAI DW, NIE H, YAN R, et al.A Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures[J].Methods Mol Biol, 2013, 1030:151-161.
    [6]KOCK J, SCHLICHT HG.Analysis of the earliest steps of hepadnavirus replication:Genome repair after infectious entry into hepatocytes does not depend on viral polymerase activity[J].J Virol, 1993, 67 (11) :4867-4874.
    [7]ADDISON WR, WONG WW, FISCHER KP, et al.A quantitative competitive PCR assay for the covalently closed circular form of the duck hepatitis B virus[J].Antiviral Res, 2000, 48 (7) :27-37.
    [8]HE ML, WU J, CHEN Y, et al.A new and sensitive method for the quantification of HBV cccDNA by real-time PCR[J].Biochem Biophys Res Commun, 2002, 295:1102-1107.
    [9]SHAO JB, CHEN Z, NI WQ, et al.A quantitative method to detect HBV cccDNA by chimeric primer and real-time polymerase chain reaction[J].J Virol Method, 2003, 112:45-52.
    [10]WONG DK, YUEN MF, YUAN H, et al.Quantitation of covalently closed circular hepatitis B virus DNA in chronic hepatitis B patients[J].Hepatology, 2004, 40 (3) :727-737.
    [11]WERLE-LAPOSTOLLE B, BOWDEN S, ZOULIM F, et al.Persistence of HBV cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy[J].Gastroenterology, 2004, 126 (11) :1750-1758.
    [12]NASSAL M.HBV cccDNA:Viral persistence reservoir and key obstacle for a cure of chronic hepatitis B[J].Gut, 2015, 34 (1) :1-13.
    [13]LUO J, CUI X, HU J.Identification of intermediate in hepatitis B virus cccDNA.formation and sensitive and selective cccD-NA detection[J].J Virol, 2017, 8 (3) :539-556.
    [14]JIANG PX, MAO RC, ZHANG JM, et al.Exonuclease I and IIIimprove the detection efficacy of hepatitis B virus covalently closed circular DNA[J].Hepatobiliary Pancreat Dis Int, 2018, 19 (18) :261-269.
    [15]QU B, NI Y, LEMPP FA, et al.T5 exonuclease hydrolysis of hepatitis B virus replicative intermediates allows reliable quantification and fast drug efficacy testing of covalently closed circular DNA by PCR[J].J Virol, 2018, 92 (23) :e01117-e01118.
    [16]YEH CT, CHIU HT, CHU CM.G1 phase dependent nuclear localization of rcDNA and aphindicolin induced HBV cccDNA[J].J Med Virol, 1998, 55 (5) :42-50.
    [17]GUO YC, SHENG SC, NIE B, et al.Development of magnetic capture hybridization and quantitative polymerase chain reaction for hepatitis B virus covalently closed circular DNA[J].Hepat Mon, 2015, 15 (6) :729-736.
    [18]MARGERIDON S, CARROUE DS, CHEMIN I, et al.Rolling circle amplification, a powerful tool for genetic and functional studies of complete hepatitis B virus genomes from low-level infections and for directly probing covalently closed circular DNA[J].Antimicrob Agents Chemother, 2008, 52 (9) :3068-3073.
    [19]ZHONG YW, HAN JQ, ZOU ZS, et al.Quantitation of HBV covalently closed circular DNA in micro formalin fixed paraffinembedded liver tissue using rolling circle amplification in combination with real-time PCR[J].Clin Chim Acta, 2011, 412 (6) :1905-1911.
    [20]ZHONG YW, HU SY, XU C, et al.A novel method for detection of HBV cccDNA in hepatocytes using rolling circle amplification combined with in situ PCR[J].BMC infect Dis, 2014, 14:608-619.
    [21]ZHANG XN, LU W, ZHENG Y, et al.In situ analysis of intrahepatic virological events in chronic hepatitis B virus infection[J].J Clin Investig, 2016, 126 (2) :1079-1092.
    [22]WHITE RA, QUAKE SR, CURR K.Digital PCR provides absolute quantitation of viral load for an occult RNA virus[J].J Virol Methods, 2011, 179 (6) :45-50.
    [23]HINDSON BJ, NESS KD, MASQUELIER DA, et al.Highthroughput droplet digital PCR system for absolute quantitation of DNA copy number[J].Anal Chem, 2011, 83 (12) :8604-8610.
    [24]VOGELSTEIN B, KINZLER KW.Digital PCR[J].Proc Natl Acad Sci U S A, 1999, 96 (4) :9236-9241.
    [25]HAYDEN RT, GU Z, INGERSOLL J.Comparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus[J].J Clin Microbiol, 2013, 51 (11) :540-546.
    [26]MU D, YAN L, TANG H, et al.A sensitive and accurate quantification method for the detection of hepatitis B virus covalently closed circular DNA by the application of a droplet digital polymerase chain reaction amplification system[J].Biotechnol Lett, 2015, 37 (10) :2063-2073.
    [27]CAVIGLIA GP, ABATE ML, TANDOI F, et al.Quantitation of HBV cccDNA in anti-HBc-positive liver donors by droplet digital PCR:A new tool to detect occult infection[J].J Hepatol, 2018, 69 (2) :301-307.
    [28]WALLRAPP A, RIESENFELD SJ, BURKETT PR, et al.The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation[J].Nature, 2017, 549 (7672) :351-356.
    [29]YILMAZ S, SINGH AK.Single cell genome sequencing[J].Curr Opin Biotechnol, 2012, 23 (3) :437-443.
    [30]ZONG CZ, LU SJ, CHAPMAN AR, et al.Genome-wide detection of single nucleotide and copy number variations of a single human Cell[J].Science, 2012, 338 (6114) :1622-1626.
    [31]LU SJ, ZONG CZ, XIE XS, et al.Probing meiotic recombination and aneuploidy of single sperm cells by whole genome sequencing using MALBAC[J].Science, 2012, 338 (6114) :1627-1630.
    [32]HUANG JT, YANG Y, LIU SM, et al.A highly sensitive and robust method for hepatitis B virus covalently closed circular DNA detection in single cells and serum[J].J Mol, 2018, 20 (3) :334-343.
    [33]ZOULIM F.New insight on hepatitis B virus persistence from the study of intrahepatic viral HBV cccDNA[J].J Hepatol, 2005, 42 (3) :302-308.
    [34]LUCIFORA J, SALVETTI A, MARNIQUET X, et al.Detection of the hepatitis B virus (HBV) covalently-closed-circular DNA (HBV cccDNA) in mice transduced with a recombinant AAV-HBV vector[J].Antiviral Res, 2017, 145 (9) :14-19.
    [35]YUEN MF, WONG DK, SUM SS, et al.Effect of lamivudine therapy on the serum covalently closed-circular (ccc) DNAof chronic hepatitis B infection[J].Am J Gastroenterol, 2005, 100 (5) :1099-1103.
    [36]CHEN YJ, HE ML.HBV cccDNA in patients'sera as an indicator for HBV reactivation and an early signal of liver damage[J].World J Gastroenterol, 2004, 10 (1) :82-85.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2763) PDF downloads(465) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return