Association of TRIB1 rs2954029 single nucleotide polymorphism with the risk of nonalcoholic fatty liver disease and coronary heart disease

Objective To investigate the association of TRIB1 rs2954029 single nucleotide polymorphism with nonalcoholic fatty liver disease (NAFLD) and coronary heart disease (CHD) and its influence on blood lipids in the Chinese Han population in Qingdao, China.Methods A total of 146 patients with NAFLD, 155 patients with CHD, and 156 patients with NAFLD and CHD, who were admitted to Qingdao Municipal Hospital from January to November, 2017, were enrolled as NAFLD group, CHD group, and NAFLD + CHD group, respectively, and 175 healthy individuals were enrolled as control group. Fasting venous blood samples were collected for biochemical analysis and TRIB1 rs2954029 genotyping. The chi-square test was used to analyze whether the distribution of genotypes was in accordance with the Hardy-Weinberg equilibrium. The chi-square test was used for comparison of categorical data between groups; the t-test and the Wilcoxon rank-sum test were used for comparison of continuous data between groups. The unconditioned logistic regression model was used to calculate odds ratio (OR) and its 95% confidence interval (CI) . Results There were no significant differences in rs2954029 genotype/allele frequency and distribution between the CHD group and the control group, as well as between the NAFLD + CHD group and the NAFLD group (all P > 0. 05) . In the CHD group and the control group, rs2954029 AT + AA carriers did not have an increased risk of CHD compared with rs2954029 TT carriers (OR = 1. 496, 95% CI: 0. 947-2. 363, P = 0. 084) ; in the NAFLD + CHD group and the NAFLD group, rs2954029 AT + AA carriers did not have an increased risk of CHD compared with rs2954029 TT carriers (OR = 1. 361, 95% CI: 0. 832-2. 227, P =0. 219) ; there were still no significant differences after adjustment for sex, age, and body mass index. In the whole population and the NAFLD group, there were no significant differences in biochemical parameters between the individuals carrying A allele and those not carrying this allele (P > 0. 05) ; in the CHD group and the NAFLD + CHD group, the individuals carrying A allele had a significantly higher level of triglyceride than those not carrying this allele (Z =-1. 986, -2. 521, all P < 0. 05) ; in the control group, the individuals carrying A allele had a significantly higher level of total cholesterol than those not carrying this allele (Z =-2. 653, P < 0. 05) . Conclusion In the Chinese Han population in Qingdao, TRIB1 rs2954029 single nucleotide polymorphism does not increase the risk of CHD in NAFLD patients.