Effect of interferon-α and thymopentin on the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells

Objective To investigate the effect of synergistic intervention of interferonα(IFNα) and thymopentin(TP5) on the mRNA expression of apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3 A(APOBEC3 A) and apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3 B(APOBEC3 B) in HepG2. 2. 15 cells. Methods HepG2. 2. 15 cells were divided into blank control group,IFNα treatment group,TP5 treatment group,and IFNα + TP5 treatment group,and at 12,24,48,and 72 hours of treatment,quantitative real-time PCR was used to measure the mRNA expression of APOBEC3 A and APOBEC3 B in HepG2. 2. 15 cells. An analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results Compared with the blank control group,the IFNα treatment group and the IFNα + TP5 treatment group had a significant increase in the mRNA expression of APOBEC3 A at 12,24,48,and 72 hours of treatment(all P < 0. 001). Compared with the IFNα treatment group,the IFNα + TP5 treatment group had a significant increase in the mRNA expression of APOBEC3 A at these four time points(all P < 0. 001). TP5 treatment had no significant influence on the mRNA expression of APOBEC3 A at each time point(all P > 0. 05). There was no significant difference in the mRNA expression of APOBEC3 B between the blank control group and the treatment groups(all P > 0. 05). Conclusion IFNα combined with TP5 can significantly upregulate the mRNA expression of APOBEC3 A in HepG2. 2. 15 cells.