Effect of extracellular ubiquitin on the proliferation,invasion,and migration of hepatoma cells

Objective To investigate the effect of extracellular ubiquitin on the proliferation,invasion,and migration of hepatoma cells by in vitro cell experiments. Methods The hepatoma cells( HepG2) were treated with extracellular ubiquitin at different concentrations( 200,400,and 800 ng/ml); CCK-8 assay was used to observe cell proliferation,Transwell assay was used to observe the effect of extracellular ubiquitin at different concentrations on the invasion ability of hepatoma cells,and wound healing assay and Western blotting were used to observe the effect of extracellular ubiquitin on the migration ability of hepatoma cells. A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups.Results The CCK-8 assay showed that extracellular ubiquitin significantly promoted the proliferation of hepatoma cells in a concentration-dependent manner,with the most significant effect at the concentration of 400 ng/ml,and the intervention group had a significantly higher relative absorbance value than the control group at 48,72,and 96 hours( all P < 0. 05). The Transwell assay showed that different concentrations of extracellular ubiquitin significantly promoted the invasion of hepatoma cells,with the most significant effect in the group treated by400 ng/ml extracellular ubiquitin( 134. 00 ± 8. 18 vs 347. 33 ± 18. 90,t = 17. 94,P < 0. 001). The Wound healing assay and Western blotting showed that 400 ng/ml extracellular ubiquitin significantly increased the migration ability of HepG2 cells. Conclusion Extracellular ubiquitin can significantly promote the proliferation,invasion,and migration of HepG2 cells in vitro in a concentration-dependent manner.