Objective To investigate the association between microRNA-155( miR-155)/SH2-containing inositol phosphatase-1( SHIP-1) and cardiovascular events in patients with nonalcoholic fatty liver disease( NAFLD). Methods A total of 104 patients with NAFLD who were admitted to our hospital from March 2018 to July 2019 were enrolled as NAFLD group,and 110 healthy volunteers who underwent physical examination in our hospital during the same period of time were enrolled as control group. Related indices were measured,including body mass index( BMI),blood pressure,fasting plasma glucose( FPG),total cholesterol( TC),triglyceride( TG),low-density lipoprotein cholesterol( LDL-C),high-density lipoprotein cholesterol( HDL-C),alanine aminotransferase( ALT),aspartate aminotransferase( AST),gamma-glutamyl transpeptidase( GGT),and total bilirubin( TBil),and quantitative real-time PCR was used to measure the mRNA expression of miR-155 and SHIP-1 in peripheral blood. The t-test was used for comparison of continuous data between two groups,the chi-square test was used for comparison of categorical data between two groups,and the Kruskal-Wallis H test was used for comparison of ranked data between groups. A Pearson correlation analysis was performed,and the receiver operating characteristic( ROC) curve was used to investigate the value of miR-155/SHIP-1 ratio in predicting cardiovascular events. Results Compared with the control group,the NAFLD group had significantly higher levels of BMI,FPG,TC,TG,LDL-C,ALT,AST,GGT,and TBil and a significantly lower level of HDL-C,as well as significantly higher relative mRNA expression of miR-155,significantly lower relative mRNA expression of SHIP-1,and a significantly higher incidence rate of cardiovascular events( t = 6. 617,9. 323,6. 668,8. 633,4. 285,22. 099,24. 093,20. 438,12. 366,6. 515,18. 893,18. 411,and 29. 967,χ2= 10. 476,all P < 0. 01). The patients were divided into three groups according to miR-155/SHIP-1 ratio,there were significant differences between the three groups in BMI,FPG,TC,TG,LDL-C,HDL-C,ALT,AST,GGT,TBil,and incidence rate of cardiovascular events( H = 20. 923,7. 936,6. 256,16. 181,21. 572,8. 435,18. 912,22. 869,7. 665,18. 657,and 9. 701,all P < 0. 05). The miR-155/SHIP-1 ratio was significantly correlated with BMI,FPG,TC,TG,LDL-C,HDL-C,ALT,AST,GGT,and TBil( r = 0. 297,0. 317,0. 332,0. 327,0. 286,-0. 279,0. 334,0. 352,0. 342,and 0. 350,all P < 0. 001). The patients with cardiovascular events had a significantly higher miR-155/SHIP-1 ratio than those without cardiovascular events( 3. 642 ± 1. 082 vs 2. 237 ± 0. 703,t = 11. 104,P < 0. 05),and the miR-155/SHIP-1 ratio had an area under the ROC curve of 0. 902 in predicting cardiovascular events in patients with NAFLD. Conclusion Abnormal glycolipid metabolism and liver function in patients with NAFLD are closely associated with the expression of miR-155/SHIP-1,and miR-155/SHIP-1 ratio has a certain value in predicting cardiovascular events in patients with NAFLD.
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