Effect of Quzhi Ruangan decoction on the mRNA and protein expression of organic anion transporting polypeptide 2B1 in the small intestine of rats with nonalcoholic fatty liver disease

ZHANG SuYan; XIA EnRui; ZHOU QingLi; ZHANG ShunZhen

doi:10.3969/j.issn.1001-5256.2020.11.017

Volume 36 Issue 11

Nov. 2020

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Article Navigation > Journal of Clinical Hepatology > 2020 > 36(11): 2484-2488

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Effect of Quzhi Ruangan decoction on the mRNA and protein expression of organic anion transporting polypeptide 2B1 in the small intestine of rats with nonalcoholic fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2020.11.017

School of Chinese Materia Medica,Yunnan University of Chinese Medicine
School of Basic Medical Sciences,Yunnan University of Chinese Medicine

Research funding:

Received Date: 2020-04-30
Published Date: 2020-11-20

Abstract

Abstract

Objective To investigate the effect of Quzhi Ruangan decoction on the mRNA and protein expression of organic anion transporting polypeptide 2 B1( OATP2 B1) in the small intestine of rats with nonalcoholic fatty liver disease( NAFLD). Methods After 1 week of adaptive feeding,36 male Sprague-Dawley rats were randomly divided into normal group,model group,simvastatin group,and high-,middle-,and low-dose Quzhi Ruangan decoction groups,with 6 rats in each group. Liver tissue was collected and HE staining was used to observe hepatic steatosis; an automatic biochemical analyzer was used to measure the serum levels of alanine aminotransferase( ALT),aspartate aminotransferase( AST),glucose( GLU),and cholesterol( CHOL); RT-PCR and Western blot were used to measure the mRNA and protein expression of OATP2 B1 in the small intestine of rats. A one-way analysis of variance was used for comparison of continuous data between multiple groups,and the least significant difference t-test was used for further comparison between two groups. Results There were significant differences in liver index,GLU,and CHOL between groups( F = 10. 814,12. 298,and 5. 024,all P < 0. 05),and there were also significant differences in the mRNA and protein expression of OATP2 B1 in the small intestine( F = 13. 384 and 73. 025,both P<0. 05). Compared with the normal group,the model group had significant increases in the mRNA and protein expression of OATP2 B1( both P < 0. 05); compared with the model group,the simvastatin group and the high-and middle-dose Quzhi Ruangan groups had a significant reduction in the mRNA expression of OATP2 B1( all P < 0. 05); compared with the simvastatin group,the middle-and low-dose Quzhi Ruangan groups had a significant reduction in the mRNA expression of OATP2 B1( both P < 0. 05). Compared with the normal group,the model group had a significant increase in the protein expression of OATP2 B1( P < 0. 05); compared with the model group,all treatment groups had a significant reduction in the protein expression of OATP2 B1( all P < 0. 001); compared with the simvastatin group,the high-,middle-,and low-dose Quzhi Ruangan groups had a significant reduction in the protein expression of OATP2 B1( all P < 0. 05). Conclusion Quzhi Ruangan decoction may alleviate the pathological changes of NAFLD by reducing the overexpression of OATP2 B1.
- non-alcoholic fatty liver disease,
- organic anion transporters,
- rats,Sprague-Dawley

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References

[1] SHU JR,LI JQ,LIU Q. Epidemiology of nonalcoholic fatty liver disease and related risk factors[J]. J Clin Hepatol,2019,35(9):2085-2090.(in Chinese)舒筠然，李俊琪，刘琼．非酒精性脂肪性肝病的流行病学和危险因素分析[J]．临床肝胆病杂志，2019,35(9):2085-2090.

[2] National Workshop on Fatty Liver and Alcoholic Liver Disease,Chinese Society of Hepatology,Chinese Medical Association,Fatty Liver Expert Committee,Chinese Medical Doctor Association. Guideline of prevention and treatment for nonalcoholic fatty liver disease:A 2018 update[J]. J Clin Hepatol,2018,34(5):947-957.(in Chinese)中华医学会肝脏病学分会脂肪肝和酒精性肝病学组，中国医师协会脂肪性肝病专家委员会．非酒精性脂肪性肝病防治指南(2018更新版)[J]．临床肝胆病杂志，2018,34(5):947-957.

[3] LI ZZ,XUE J,CHEN P,et al. Prevalence of nonalcoholic fatty liver disease in mainland of China:A meta-analysis of published studies.[J]. J Gastroenterol Hepatol,2014,29(1):42-51.

[4] DUAN SJ,CHEN JL,CHEN J,et al. Research progress of correlation between nonalcoholic fatty liver disease and metabolic syndrome in traditional chinese medicine and western medicine[J]. Med Recapitulate,2019,25(15):2935-2939.(in Chinese)段绍杰，陈佳良，陈洁，等．非酒精性脂肪性肝病与代谢综合征的中西医相关性研究进展[J]．医学综述，2019,25(15):2935-2939.

[5] ZHOU Y,JIANG YW,ZHENG PY. Research progress of traditional Chinese medicine in treatment of nonalcoholic fatty liver disease[J]. Liaoning Tradit Chin Med,2019,46(6):1327-1330.(in Chinese)周盐，蒋雨薇，郑培永．中医药治疗非酒精性脂肪肝病临床研究进展[J]．辽宁中医杂志，2019,46(6):1327-1330.

[6] LU J,JIANG Y,ZHANG X,et al. Research progress of nonalcoholic fatty liver disease in traditional Chinese medicine[J].Shanghai J Tradit Chin Med,2017,51(S1):238-241.(in Chinese)吕佳，江云，张鑫，等．非酒精性脂肪性肝病中医研究进展[J]．上海中医药杂志，2017,51(S1):238-241.

[7] YU X,WANG WJ,JIN JY,et al. Linggui Zhugan Decoction combined with probiotics in the treatment of nonalcoholic fatty liver disease[J]. J Changchun Univ Chin Med,2019,35(5):891-894.(in Chinese)喻晓，王雯婕，金嘉悦，等．苓桂术甘汤联合益生菌治疗非酒精性脂肪肝[J]．长春中医药大学学报，2019,35(5):891-894.

[8] HONG Z,CHEN SY,YANG WJ,et al. Role of dietary intervention in nonalcoholic fatty liver disease[J]. J Clin Hepatol,2016,32(3):561-564.(in Chinese)洪泽，陈思颖，杨文娟，等．膳食营养摄入对非酒精性脂肪性肝病的影响[J]．临床肝胆病杂志，2016,32(3):561-564.

[9] BAI YN. Meticulous study on the physiological function of small intestine[J]. Shanxi J Tradit Chin Med,2005,21(1):3-5.(in Chinese)白宇宁．小肠生理功能探微[J]．山西中医，2005,21(1):3-5.

[10] WEI GC,HE JY. Traditional Chinese medicine intervention to nonalcoholic fatty liver disease based on physique identification[J]. J Changchun Univ Chin Med,2018,34(3):518-521.(in Chinese)魏功昌，何瑾瑜．中医体质辨识治疗非酒精性脂肪性肝病[J]．长春中医药大学学报，2018,34(3):518-521.

[11] BAI SY,ZHANG Y,FANG RJ,et al. Syndrome and treatment analysis on water metabolism disorders and diarrhea[J]. Acta Chin Med,2017,32(2):250-252.(in Chinese)白少玉，张怡，方锐洁，等．水液代谢失常与泄泻证治探析[J]．中医学报，2017,32(2):250-252.

[12] XIANG T,REN B,YANG ZB,et al. Explorling the correlation between Pi and Shen from the expressions of organic anion transporting polypeptide 2a1 and 2b1 in Pi deficiency model Rats[J]. Chin J Integr Med,2015,35(10):1255-1260.(in Chinese)项婷，任斌，杨璋斌，等．从脾虚大鼠AA-I的排泄与脏腑oatp2a1、oatp2b1的表达探讨脾肾关系[J]．中国中西医结合杂志，2015,35(10):1255-1260.

[13] XIANG T,YANG ZB,SUN BG,et al. Exploration on the nature of sleen govering transportian and transformation based on the dynamic expression of oatp2a1 in spleen-deficiency rats[J].Chin J Tradit Chin Med and Pharm,2014,29(2):430-434.(in Chinese)项婷，杨璋斌，孙保国，等．基于脾虚大鼠有机阴离子转运肽2a1动态表达的脾主运化内涵探讨[J]．中华中医药杂志，2014,29(2):430-434.

[14] CHEN Y,DAI M,XIE HP,et al. Study on relationship between internal environment of spleen deficiency and liver cancer from expression of Oatp2a1[J]. Chin J Tradit Chin Med and Pharm,2017,32(11):5056-5059.(in Chinese)陈燕，戴敏，谢和平，等．基于Oatp2a1表达探讨小鼠脾虚内环境与肝癌的关系[J]．中华中医药杂志，2017,32(11):5056-5059.

[15] GAO X. Explorration of the relationship between the nonalcoholic fatty liver disease in rats and the correlation of dampness and blood stasis based on the QATP2a1 and OATP2b1[D].Kunming:Yunnan University of Traditional Chinese Medicine,2019.(in Chinese)高歆．基于OATP2a1、OATP2b1研究非酒精性脂肪肝大鼠与湿浊的关系[D]．昆明:云南中医药大学，2019.

[16] MCFEELY SJ,WU L,RITCHIE TK,et al. Organic anion transporting polypeptide 2B1-More than a glass-full of drug interactions[J]. Pharmacol Ther,2019,196:204-215.

[17] ZHANG SZ,SHI AH,BIAN Y,et al. Influence of Quzhi Ruangan Formula on blood fat and hemorheology of rats with early[J]. Chin J Tradit Chin Med and Pharm,2015,30(3):875-877.(in Chinese)张顺贞，石安华，卞瑶，等．去脂软肝方对早期脂肪肝大鼠血脂及血液流变学的影响[J]．中华中医药杂志，2015,30(3):875-877.

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Effect of Quzhi Ruangan decoction on the mRNA and protein expression of organic anion transporting polypeptide 2B1 in the small intestine of rats with nonalcoholic fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2020.11.017