Preliminary study of HCV mouse model induced by infectious HCV 2a plasmid

Objective To establish a mouse model which can produce hepatitis C virus(HCV) RNA for examination of the efficiency of virus clearance of high-affinity HCV monoclonal antibody in vivo.Methods Huh7-Jc1 cell model was established by transfecting a genotype 2a HCV plasmid Jc1 into Huh7 cell line which can produce HCV RNA in vitro.The Huh7-Jc1 cell was injected into the nude mouse abdominally or intravenously and/,and the remaining HCV RNA level was detected by optical in vivo imaging and virus RNA in peripheral blood was also detected.Negative control was established by injecting non-HCV Huh7 cell.Results A HCV cell model was estabished which could express HCV RNA and core protein.and the supernatant of the HCV cell model also had the ability to infect the Huh7 cells.And the HCV RNA could be detected in the serum of the BALB/c-nu nude mouse which were implanted with Huh7-Jc1 cell(intraperitoneal or tail intravenous).Conclusion Our data indicates that implanting Huh7-Jc1 cell is a feasible method for building a HCV expressing mouse model,however,a higher efficiency and long-term virus producing mouse model still needed moreresearch.