Expression of cysteinyl leukotriene receptor-1 in pancreatic cancer tissue and its clinical significance

Objective To investigate the expression of cysteinyl leukotriene receptor- 1( Cys LTR- 1) in pancreatic cancer tissue and its association with clinical features and prognosis. Methods A total of 42 patients with pathologically confirmed pancreatic cancer who were admitted to Central Hospital of Huludao from January to October,2015 were enrolled,and cancer and adjacent tissues were collected. Real-time PCR was used to measure the relative mRNA expression of Cys LTR- 1 in these tissues,and the patients were followed up for 1 year.The t- test was used for comparison of continuous data between groups; an analysis of variance was used for comparison between multiple groups,and the least significant difference t- test was used for comparison between any two groups. The multivariate logistic regression analysis was used to determine influential factors for the prognosis of patients with pancreatic cancer. Results The cancer tissues had significantly higher relative mRNA expression of Cys LTR- 1 than adjacent tissues( 0. 037 1 ± 0. 020 5 vs 0. 016 8 ± 0. 011 4,t = 6. 58,P<0. 001). The relative mRNA expression of Cys LTR- 1 was associated with TNM stage( t = 2. 731,P = 0. 009),degree of tumor differentiation( F = 10. 537,P < 0. 001),and lymph node metastasis( t = 3. 408,P = 0. 002). During the 1- year follow- up,17 patients died and25 survived; the patients who died had significantly higher relative mRNA expression of Cys LTR- 1 than those who survived( 0. 045 6 ±0. 021 7 vs 0. 031 3 ± 0. 019 8,t = 2. 21,P = 0. 03). The logistic regression analysis showed that TNM stage( OR = 6. 92,95% CI: 1. 30-36. 82,P = 0. 02),degree of tumor differentiation( OR = 5. 71,95% CI: 1. 44- 22. 62,P = 0. 01),and lymph node metastasis( OR =9. 62,95% CI: 2. 23- 41. 46,P < 0. 001) were prognostic factors for pancreatic cancer. After adjustment for age,sex,TNM stage,degree of tumor differentiation,tumor location,and lymph node metastasis,the mRNA expression of Cys LTR- 1 was still a prognostic factor for patients with pancreatic cancer( OR = 1. 11,95% CI: 1. 07- 1. 15,P = 0. 03). Conclusion Cys LTR- 1 is involved in the development and progression of pancreatic cancer and can be used as a new molecular marker for prognostic evaluation in patients with pancreatic cancer.