中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 10
Oct.  2018
Turn off MathJax
Article Contents

Expression of D-bifunctional protein in hepatocellular carcinoma tissue and its correlation with alpha-fetoprotein and carbohydrate antigen 19-9

DOI: 10.3969/j.issn.1001-5256.2018.10.018
Research funding:

 

  • Published Date: 2018-10-20
  • Objective To investigate the expression of D-bifunctional protein ( DBP) in hepatocellular carcinoma ( HCC) tissue and its correlation with serum tumor markers alpha-fetoprotein ( AFP) and carbohydrate antigen 19-9 ( CA19-9) , as well as the clinical value of DBP in early diagnosis and prognostic evaluation of HCC. Methods A total of 20 patients who underwent hepatectomy for liver cancer in Tangshan Maternal and Child Health Hospital and Tangshan People's Hospital from June 2015 to May 2017 were enrolled as experimental group, and 20 healthy subjects who underwent physical examination in these two hospital during the same period of time were enrolled as control group. Chemiluminescence was used to measure the expression of AFP and CA19-9, and immunohistochemistry was used to measure the expression of DBP, which was expressed as integrated optical density ( IOD) , in HCC tissue and adjacent tissue. The t-test was used for comparison of continuous data between groups, and the Pearson correlation analysis was used to investigate correlation between indices. Results The IOD value of DBP in HCC tissue was significantly higher than that in adjacent tissues ( 220. 52 ± 101. 30 vs 40. 49 ±19. 32, t =-7. 00, P < 0. 01) . The expression of DBP was positively correlated with that of AFP ( r2= 0. 868, P < 0. 01) and CA19-9 ( r2= 0. 814, P < 0. 01) . Conclusion The expression of DBP increases in HCC patients, which is closely associated with the development and progression of HCC. Therefore, it provides a new target for the diagnosis of HCC.

     

  • loading
  • [1]El-SERAG HB.Hepatocellular carcinoma[J].N Engl J Med, 2011, 365 (12) :1118-1127.
    [2]GHOURI Y, MIAN I, ROWE JH.Review of hepatocellular carcinoma:epidemiology, etiology, and carcinogenesis[J].J Carcinog, 2017, 16:1.
    [3]MOELLER G, ADAMSKI J.Multifunctionality of human 17betahydroxysteroid dehydrogenases[J].Mol Cell Endocrinol, 2006, 248:47-55.
    [4]LU X, MA PP, SHI Y, et al.NF-κB increased expression of 17β-hydroxysteroid dehydrogenase 4promotes HepG2 proliferation via inactivating estradiol[J].Mol Cell Endocrinol, 2015, 401:1-11.
    [5]LIU YW, WU XM.Diagnostic and prognostic significance of AFP, CA199 and CEA levels in patients with hepatocellular carcinoma[J].Lab Med, 2017, 32 (5) :406-409. (in Chinese) 刘永炜, 吴向民.血清AFP、CA199和CEA水平在肝癌诊断和预后中的作用[J].检验医学, 2017, 32 (5) :406-409.
    [6]WANG DX, QUAN WJ, SHI XX, et al.Research of the markers and their detection methods for early diagnosis of hepatocellular carcinoma[J].Anti-Tumor Pharm, 2016, 6 (1) :15-20. (in Chinese) 王丹霞, 全文婕, 史晓欣, 等.肝癌早期诊断标志物及其检测方法的研究进展[J].肿瘤药学, 2016, 6 (1) :15-20.
    [7]CZ HE, KH ZHANG, Q LI, et al.Combined use of AFP, CEA, CA125 and CAl9-9 improves the sensitivity for the diagnosis of gastric cancer[J].BMC Gastroenterol, 2013, 13:87.
    [8]JIA HL, XING XJ, YE QH, et al.Application of alpha-fetoprotein in the diagnosis of hepatocellular carcinoma[J].Acta Acad Med Sin, 2008, 30 (4) :440-443. (in Chinese) 贾户亮, 刑戌健, 叶青海, 等.甲胎蛋白在原发性肝癌临床诊断中的应用[J].中国医学科学院学报, 2008, 30 (4) :440-443.
    [9]SUN Y, GAO G, CAI J, et al.AnnexinA2 is a discriminative serologi-calcandidate in early hepatocellular carcinoma[J].Carcinogenesis, 2013, 34 (2) :595.
    [10]LI QR, JI SD.Application of multiple tumor marker protein chip in healthy screening of old people and its clinical significance[J].JJilin Univ:Med Edit, 2016, 42 (3) :535-540. (in Chinese) 李清茹, 季顺东.多肿瘤标志物蛋白芯片检测在老年人健康体检中的应用价值及其临床意义[J].吉林大学学报:医学版, 2016, 42 (3) :535-540.
    [11]HAN ZX, GONG J, ZHENG Y.Combined detection of serum carbohydrate antigens and CEA levels in patients with pancreatic cancer[J].Chin J Med Offic, 2016, 44 (10) :1052-1054. (in Chinese) 韩忠学, 巩静, 郑艳.血清糖类抗原与癌抗原对胰腺癌诊断价值[J].临床军医杂志, 2016, 44 (10) :1052-1054.
    [12]DAI WW, LIU ZX, XU BH.Serum CA125, CA199, AFP, CEA in patients with cirrhosis and primary liver cancer[J].J Pract Hepatol, 2017, 20 (1) :81-84. (in Chinese) 代伟伟, 刘正新, 徐宝宏.肝硬化和肝癌患者血清CA125、CA199、AFP和CEA水平变化[J].实用肝脏病杂志, 2017, 20 (1) :81-84.
    [13]MA HM, TU X.Value of joint detection of AFP, CEA, and CA199in diagnosis for primary live cancer[J].Int J Lab Med, 2016, 37 (12) :1663-1664. (in Chinese) 马海梅, 涂秀.AFP、CEA、CA199联合检测在原发性肝癌中的诊断价值[J].国际检验医学杂志, 2016, 37 (12) :1663-1664.
    [14]ZHU QH.Diagnostic value of joint detection of golgin 73, alphafetoprotein, alpha-fetoprotein variants for primary hepatocellular carcinoma[J].China Med Herald, 2016, 13 (5) :130-133. (in Chinese) 诸琴红.高尔基体蛋白73、甲胎蛋白、甲胎蛋白异质体3%联合检测对原发性肝癌的诊断价值[J].中国医药导报, 2016, 13 (5) :130-133.
    [15]ZHANG Y, XU YY, YAO CB, et al.Acetylation targets HSD17B4 for degradation viathe CMA pathway in response to estrone[J].Autophagy, 2017, 13 (3) :1-16.
    [16]YANG W, LU Y, XU Y, et al.Estrogen represses hepatocellular carcinoma (HCC) growth via inhibiting alternative activation of tumor-associated macrophages (TAMs) [J].J Biol Chem, 2012, 287:40140-40149.
    [17]RASIAH KK, GARDINER-G M, PADILLA EJ, et al.DBP overexpression, an independent biomarker of poor patient outcome in prostate cancer[J].Mol Cell Endocrinol, 2009, 301:89-96.
    [18]SHI L, FENG Y, LIN H, et al.Role of estrogen in hepatocellular carcinoma:Is inflammation the key?[J].J Transl Med, 2014, 12:93.
    [19]NAGAYOSHI Y, OHBA T, YAMAMOTO H, et al.Characterization of 17beta-hydroxysteroid dehydrogenase type 4 in human ovarian surface epithelial cells[J].Mol Hum Reprod, 2005, 11:615-621.
    [20]MALEKI J, NOURBAKHSH M, SHABANI M, et al.17beta-estradiol stimulates generation of reactive species oxygen and nitric oxide in ovarian adenocarcinoma cells (OVCAR3) [J].Iran J Cancer Preven, 2015, 8 (3) :e2332.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1928) PDF downloads(353) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return