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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 36 Issue 9
Sep.  2020
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Article Contents

Mechanism of action of Sini powder in treatment of liver cancer based on network pharmacology and molecular docking

DOI: 10.3969/j.issn.1001-5256.2020.09.018
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  • Received Date: 2020-02-24
  • Published Date: 2020-09-20
  • Objective To investigate the mechanism of action of Sini powder in the treatment of liver cancer based on network pharmacology and molecular docking. Methods Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was used to obtain the compound and target of Sini powder,and the corresponding gene Symbol was obtained through Uniprot. The disease genes of liver cancer were obtained from Human Genome Database,and the genes with intersection with the target genes of Sini powder were screened out. Cytoscape3. 7. 1 software was used to draw the map of“traditional Chinese medicine( TCM)-compound-target”network. STRING was used to construct a protein-protein interaction( PPI) network,R studio software was used to conduct gene ontology( GO) and Kyoto Encyclopedia of Genes and Genomes( KEGG) enrichment analyses on therapeutic targets,and then the results were visualized. The active component with the highest number of targets was selected as the ligand,and the target with the highest degree in the PPI network was selected as the receptor,so as to predict the structure of receptor-ligand complex and the amino acid residues that bind to each other. Results In this study,91 core targets and 141 relevant active components of Sini powder were screened out,among which quercetin and kaempferol were the main active components in the treatment of liver cancer. TP53 and HSP90 AA1 were the main therapeutic targets. The GO enrichment analysis obtained 1007 items which met the screening criteria,which were mainly involved in the biological processes of antioxidation reaction,activity regulation of protein serine and threonine kinase,and cellular stress response. The KEGG enrichment analysis obtained 102 pathways,which mainly regulated the hepatitis B pathway and the PI3 K-Akt signaling pathway in the prevention and treatment of liver cancer. The results of molecular docking showed a synergistic antitumor effect between the crystal structure domains VAL147,CYS220,GLU221,and PRO222 of quercetin-TP53. Conclusion This study reveals the mechanism of action of Sini powder in the treatment of liver cancer by acting on multiple targets and signaling pathways,which provides a theoretical basis for biological experiments.

     

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