Analysis of differential gene expression profiles in hepatic fibrosis tissue using cDNA microarray

Wu ShiPin; Liu ZhenZhen; Zhou Jun; Huang MingHui

Issue 4

Apr. 2007

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Article Navigation > Journal of Clinical Hepatology > 2007 > 23(4): 258-260

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DNA微阵矩技术研究肝纤维化差异基因表达谱变化

Published Date: 2007-04-20

Abstract

Abstract

To investigate the differentially expressed genes in hepatic fibrosis tissues using cDNA microarray and screen for fibrosis-related genes.Total RNA was extracted from normal human liver tissues and liver cirrhosis tissues.The mRNA was used to prepare probes.The mixed probes were hybridized to the cDNA microarray.After washing, the DNA chips were scanned by laster scanner.The acquired image was analyzed.Among the 10.000 target genes, 99 genes were identified in liver cirrhosis tissues that were either over or under-expressed as compared to controls.45 genes were up-regulated and 54 down-regulated.9 new genes were found in the study.The results showed that the cDNA microarray for analysis of gene expression profile is a powerful method for screening hepatic fibrosis-related genes.
- oligonucleotide array sequence analysis,
- gene expression,
- liver cirrhosis

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沈阳化工大学材料科学与工程学院沈阳 110142

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DNA微阵矩技术研究肝纤维化差异基因表达谱变化

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DNA微阵矩技术研究肝纤维化差异基因表达谱变化

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