Packaging, selection and identification of recombinant adenoviruses carrying HCV HLA-A2 restricted compound multi-epitopes antigen

Wei SanHua; Ren JiHong; Liu Li; Jia ZhanSheng; Dong Ke; Lin Fang; Wang Xi; Zhang LiJun; Liu XinYang; Zhang HuiZhong

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Jan. 2011

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Packaging, selection and identification of recombinant adenoviruses carrying HCV HLA-A2 restricted compound multi-epitopes antigen

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Published Date: 2011-01-20

Abstract

Abstract

Objective To construct recombinant shuttle plasmid pAdtrack-CMV-Ub-Mep carrying HCV HLA-A2 restricted compound multi-epitopes gene, and transfect to HEK293 cells, to obtain recombinant adenoviruses rAd-Ub-Mep.Methods The compound antigen gene Ub-Mep was cloned into shuttle plasmid pAdTrack-CMV for pAdtrack-CMV-Ub-Mep.The recombinant adenoviruses rAd-Ub-Mep was packed in HEK293 cells by co-transfection with pAdtrack-CMV-Ub-Mep and frame plasmid pAdEasy1.The recombinant adenoviruses rAd-Ub-Mep was identified by PCR and expression of GFP, and the virus titer was determined by TCID50.Results The recombinant adenoviruses rAd-Ub-Mep was confirmed by enzyme cutting, PCR and fluorometric analysis.The virus titer was 1.2×1011cfu/L.Conclusion Success to construct recombinant adenoviruses vaccine, is the base for the study of cellular immunity induced by HCV HLA-A2 restricted compound multi-epitopes gene.
- hepacivirus,
- epitopes,
- adenoviruses

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沈阳化工大学材料科学与工程学院沈阳 110142

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Packaging, selection and identification of recombinant adenoviruses carrying HCV HLA-A2 restricted compound multi-epitopes antigen

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Packaging, selection and identification of recombinant adenoviruses carrying HCV HLA-A2 restricted compound multi-epitopes antigen

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