Objective To observe the effect of Ruangan Huajian granule on hepatic expression of alpha-smooth muscle actin (α-SMA) , tumor growth factor-beta 1 (TGFβ1) , and collagen I (COL-Ⅰ) using a C57 mouse model of experimental hepatic fibrosis.Methods Forty C57 mice were randomly divided into four groups (n=10 each) : uninduced normal control group, model control group, model treatment group with low-dose (0.1 g/day for six weeks) Ruangan Huajian granule, model treatment group with high-dose (0.2 g/day for six weeks) Ruangan Huajian granule.Hepatic fibrosis was induced by intraperitoneal injection of 10% carbon tetrachloride.The expression of α-SMA, TGFβ1, and COL-Ⅰ in liver tissue of mice was observed by immunohistochemistry.The liver tissues were also scored for fibrosis stage by evaluating morphological changes detected upon hematoxylin-eosin and Masson trichrome staining.Results There were significantly higher levels of α-SMA, TGFβ1, and COL-Ⅰ expression and scores of fibrosis stage in the hepatic fibrosis mice than in the normal control mice (P<0.05) and in the model control group than in either the low-dose or high-dose model treatment groups (P<0.05) .there="" was="" no="" significant="" difference="" between="" the="" low-dose="" model="" treatment="" group="" and="" high-dose="" p="">0.05) .Conclusion Ruangan Huajian granule may alleviate the degree of fibrosis in C57 mice with experimental hepatic fibrosis by reducing hepatic expression of α-SMA and TGFβ1.Furthermore, Ruangan Huajian granule may depress hyperplasia by reducing COL-Ⅰ expression.
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