Relationship between percentages of T lymphocytes in peripheral blood and deficiency of vital qi in patients with HBV- ACLF

Cao Hui; Zhang Yang; Tan ShanZhong; Zhao Lei; Shen JianJun; Xiao Qian; Liang ZhongFeng; Jiang ShuLian

doi:10.3969/j.issn.1001-5256.2014.04.009

Issue 4

Apr. 2014

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > Journal of Clinical Hepatology > 2014 > 30(4): 320-323

PDF( 1306 KB)

Relationship between percentages of T lymphocytes in peripheral blood and deficiency of vital qi in patients with HBV- ACLF

DOI: 10.3969/j.issn.1001-5256.2014.04.009

Department of Integrated , TCM & Western Medicine, The Second Hospital of Nanjing

Research funding:

Received Date: 2013-07-29
Published Date: 2014-04-20

Abstract

Abstract

Objective To investigate the relationship between percentages of T lymphocytes in peripheral blood and deficiency of vital qi in patients with hepatitis B virus- related acute- on- chronic liver failure (HBV- ACLF) .Methods Forty patients with early- stage HBV-ACLF were enrolled as liver failure group, and 50 patients with chronic hepatitis B (CHB) as CHB group;20 healthy subjects who underwent physical examination in the clinic during the same period were selected as control group.Flow cytometry was used to determine the percentages of CD3+T lymphocytes, CD4+and CD8+T lymphocytes, and CD4+CD25+regulatory T cells (Tregs) in peripheral blood, as well as CD4+/ CD8+ratio, and the three groups were compared in this regard by analysis of variance.Results Compared with the control group and CHB group, the liver failure group had a significantly decreased percentage of CD3+T lymphocytes in peripheral blood (34.12% ± 22.33% vs 50.31% ± 12.09% and 49.72% ± 20.11%, P < 0.05) , a significantly increased CD4+/ CD8+ratio (1.78 ± 1.25 vs 1.32 ± 0.31 and 1.31 ± 0.52, P < 0.05) , and a significantly decreased percentage of CD4+CD25+Tregs in peripheral blood (0.83% ± 1.00% vs 2.93% ± 1.31% and 3.17% ± 2.29%, P < 0.05) .Conclusion Once HBV- ACLF develops, T lymphocyte cells in peripheral blood and related effector T lymphocytes are“exhausted”, which reveals that the characteristics of its pathogenesis is“deficiency of vital qi”.
- liver failure,
- hepatitis B, chronic,
- T lymphocyte,
- TCM WM THERAPY

FullText(HTML)

References(16)

References

[1]YIN SP, GUO Q, JIANG Y, et al.Clinical study of lamivudine in the treatment of acute-on-chronic liver failure patients caused by hepatitis B[J].China Med Herald, 2012, 9 (17) :182-183. (in Chinese) 阴生平, 郭堑, 蒋茵, 等.拉米夫定治疗慢性乙型肝炎引起的慢加急性肝衰竭患者的临床研究[J].中国医药导报, 2012, 9 (17) :182-183.

[2]ZHANG Y, TAN SZ.The research progress in etiological and pathogenetic characteristics of Chinese medicine on chronic severe hepatitis/liver failure[J].Chin J Integr Tradit West Med Liver Dis, 2011, 21 (6) :380-383. (in Chinese) 张旸, 谭善忠.慢性重型肝炎/肝衰竭的中医病因病机特点研究进展[J].中西医结合肝病杂志, 2011, 21 (6) :380-383.

[3]SHI WL, SUN YQ, ZHANG T.The ideas and methods of triple factors Chinese medicine in mechanism of prevention and treatment on hepatitis B-liver failure[J].J Tradit Chin Med, 2011, 52 (5) :389-391. (in Chinese) 史文丽, 孙永强, 张弢.中医药防治乙型肝炎肝功能衰竭发生机制中三重因素的思路和方法[J].中医杂志, 2011, 52 (5) :389-391.

[4]ZHUANG QJ, QIU LM, YAO XS, et al.CD4+CD25+regulatory T cells and hepatitis B virus infection[J].World Chin J Dig, 2012, 20 (24) :2248-2253. (in Chinese) 庄勤建, 邱隆敏, 姚新生, 等.CD4+CD25+调节性T细胞与乙型肝炎病毒感染的研究进展[J].世界华人消化杂志, 2012, 20 (24) :2248-2253.

[5] WU YY, TAN SZ.The researches on the role of CD4+CD25+tregs in the pathogenesis of chronic hepatitis B[J].Chin J Infect Dis, 2012, 30 (9) :563-565. (in Chinese) 吴银亚, 谭善忠.CD4+CD25+调节性T淋巴细胞在慢性乙型肝炎发病机制中的作用[J].中华传染病杂志, 2012, 30 (9) :563-565.

[6]Liver Failure and Artificial Liver Group, Chinese Society of Infectious Diseases, CMA;Severe Liver Disease and Artificial Liver Group, Chinese Society of Hepatology, CMA.Guideline for diagnosis and treatment of liver failure[J/CD].Chin J Transplant:Electronic Edition, 2013, 7 (1) :48-56. (in Chinese) 中华医学会感染病学分会肝衰竭与人工肝学组, 中华医学会肝病学分会重型肝病与人工肝学组.肝衰竭诊疗指南[J/CD].中华移植杂志:电子版, 2013, 7 (1) :48-56.

[7] Chinese Society of Hepatology and Chinese Society of Infectious Diseases, Chinese Medical Association.The guideline of prevention and treatment for chronic hepatitis B (2010 version) [J].J Clin Hepatol, 2011, 27 (1) :1-15. (in Chinese) 中华医学会肝病学分会, 中华医学会感染病学分会.慢性乙型肝炎防治指南 (2010年版) [J].临床肝胆病杂志, 2011, 27 (1) :1-15.

[8]ZENG ZL, WU JL, WU JZ.Detection of proportions of lymphocyte T subsets in peripheral blood and levels of Th1/Th2 cytokines in patients with hepatis B virus infection and its dinical significance[J].J Jilin Univ:Med Edit, 2012, 38 (3) :555-558. (in Chinese) 曾志励, 吴健林, 吴继周.乙型肝炎患者外同血T淋巴细胞亚群比例和Th1/Th2细胞因子水平检测及其临床意义[J].吉林大学学报:医学版, 2012, 38 (3) :555-558.

[9]SUN XH, LIU QL, LI M, et al.The study of CD4+and CD8+T subsets in chronic hepatitis B patients[J].Chin J Cell Mol Immunol, 2011, 27 (5) :545-547. (in Chinese) 孙学华, 刘巧丽, 李曼, 等.慢性乙型肝炎患者CD4+和CD8+T细胞亚群的研究[J].细胞与分子免疫学杂志, 2011, 27 (5) :545-547.

[10]DENG M, LI MH, LIU SA, et al.Studies about the level of CD4+CD25+regulatory T cells and relation between expression of Foxp3and CD127 in peripheral blood of chronic HBV infection[J].Chinese J Exp Clin Virol, 2010, 24 (1) :21-23. (in Chinese) 邓敏, 李明慧, 刘顺爱, 等.慢性HBV感染者调节性T细胞水平及Foxp3与CD127表达关系的研究[J].中华实验和临床病毒学杂志, 2010, 24 (1) :21-23.

[11]NAN XP, ZHANG Y, YU HT, et al.Circulating CD4+CD25+high regulatory T cells and expression of PD-1 and BTLA on CD4+T cells in patients with chronic hepatitis B virus infection[J].Viral Immunol, 2010, 23 (1) :63-70.

[12]WU YY, TAN SZ, ZHAO L.et al.The changes and value of the proportion of CD4+CD25+regulatory T cells in HBeAg (+) and HBeAg (-) ASC in peripheral blood[J].J Clin Hepatol, 2012, 28 (3) :209-211. (in Chinese) 吴银亚, 谭善忠, 赵磊, 等.HBeAg (+) 与HBeAg (-) 乙型肝炎病毒携带者外周血CD4+CD25+调节性T淋巴细胞比例的变化及意义[J].临床肝胆病杂志, 2012, 28 (3) :209-211.

[13]ZHANG H, HUANG LX, CHEN CX, et al.Effect of CD4+CD25+Foxp3+regulatory T cells on acute-on-chronic liver failure caused by hepatitis B infection[J].J Southeast Univ:Med Sci Edi, 2011, 30 (2) :315-318. (in Chinese) 张慧, 黄丽霞, 陈朝霞, 等.CD4+CD25+Foxp3+Treg细胞在HBV感染所致慢加急性肝衰竭中作用[J].东南大学学报:医学版, 2011, 30 (2) :315-318.

[14]YANG J, YI P, WEI L, et al.Phenotypes and clinical significance of circulating CD4+CD25+regulatory T cells (Tregs) in patients with acute-on-chronic liver failure (ACLF) [J].J Transl Med, 2012, 10 (1) :193.

[15]SHI F, ZHANG JY, ZENG Z, et al.Skewed ratios between CD3 (+) T cells and monocytes are associated with poor prognosis in patients with HBV-related acute-on-chronic liver failure[J].Biochem Biophys Res Commun, 2010, 402 (1) :30-36.

[16]ZHANG Y, WU YY, TAN SZ, et al.Clinical observation on chronic severe hepatitis B treated by principle of strengthening vital Qi and the spleen based on the mechanism of deficiency in vital Qi[J].JETCM, 2012, 21 (6) :872-873, 892. (in Chinese) 张旸, 吴银亚, 谭善忠, 等.基于正虚机制观察益气健脾法治疗慢性乙型肝炎肝衰竭的临床疗效[J].中国中医急症, 2012, 21 (6) :872-873, 892.

Relative Articles

[1]	Yunchong WU, Yanyan YANG, Chuan LI, Xiaohuan WU, Shide LIN. Role of inflammatory cytokines in disorder of glucose metabolism in patients with liver cirrhosis[J]. Journal of Clinical Hepatology, 2024, 40(9): 1886-1890. doi: 10.12449/JCH240926
[2]	Zixiang GAO, Zhongliang SHEN, Jing LIU, Youhua XIE. Research advances in cytokines in the treatment of chronic hepatitis B[J]. Journal of Clinical Hepatology, 2022, 38(8): 1710-1715. doi: 10.3969/j.issn.1001-5256.2022.08.002
[3]	Huang ZhiQin, Huang HuiFang. Research advances in cytokines associated with primary biliary cholangitis[J]. Journal of Clinical Hepatology, 2020, 36(2): 446-450. doi: 10.3969/j.issn.1001-5256.2020.02.047
[4]	Zhang Yu, Shao YueMing, Qin TingTing, Yin Xin, Wen XiaoYu. Current research status and application value of cytokines in drug-induced liver injury[J]. Journal of Clinical Hepatology, 2020, 36(8): 1883-1886. doi: 10.3969/j.issn.1001-5256.2020.08.045
[5]	Wei XinHuan, Liu YaLi, Zhang Jing, Lin Wei. Expression and significance of cytokines in children with acute hepatitis A[J]. Journal of Clinical Hepatology, 2019, 35(9): 1942-1945. doi: 10.3969/j.issn.1001-5256.2019.09.011
[6]	Xiang Jiao, Jia XiaoDong, Lu ShanShan, Wu DaWei, Zhang Ting, Dong JinKe, Huang JiaGan, Li YinYin, Yang Bin, Xu Yong, Dong Zheng, Lu Ying, Zeng Zhen, Lu YinYing. Effect of cryoablation versus microwave ablation on clinical indices and cytokines in patients with hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2019, 35(8): 1728-1733. doi: 10.3969/j.issn.1001-5256.2019.08.016
[7]	Xu Ying, Zhang DingQi, Chen JiaMei, Liu Wei, Liu Ping. Effect of various cells on the activation of hepatic stellate cells in liver microenvironment[J]. Journal of Clinical Hepatology, 2019, 35(2): 424-430. doi: 10.3969/j.issn.1001-5256.2019.02.042
[8]	Liu Zhao, Ma WenTing, Zhang PengFei, Li JinKui, Xiao JianXi, Lei JunQiang. Research advances in molecular imaging of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2018, 34(4): 872-876. doi: 10.3969/j.issn.1001-5256.2018.04.038
[9]	Wu Han, Li ZhenLi, Xing Hao, Wang MingDa, Wu MengChao, Yang Tian. Research advances in the structure and function of osteopontin and its effect in diagnosis and treatment of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2018, 34(9): 1999-2003. doi: 10.3969/j.issn.1001-5256.2018.09.036
[10]	Huang YongJie, Wu DeQuan, Yuan JunHui. Current status of treatment of primary hepatocellular carcinoma in the elderly population[J]. Journal of Clinical Hepatology, 2018, 34(6): 1313-1317. doi: 10.3969/j.issn.1001-5256.2018.06.039
[11]	Sheng QingShou, Lai GuoQuan, Yu XueJing, Wei Cui. The role of traditional Chinese medicine in regulating cytokines associated with liver fibrosis[J]. Journal of Clinical Hepatology, 2018, 34(12): 2677-2680. doi: 10.3969/j.issn.1001-5256.2018.12.037
[12]	Zhang ZeYu, Cheng HongYan. Clinical application of positron emission tomography/computed tomography in hepatocellular carcinoma and related research advances[J]. Journal of Clinical Hepatology, 2017, 33(7): 1365-1368. doi: doi:10.3969/j.issn.1001-5256.2017.07.036
[13]	Chen WeiBo, Cai HuiHua, Yang Yue, Chen XueMin, Jiang Yong, Sun DongLin. Research advances in regorafenib in treatment of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2017, 33(12): 2425-2428. doi: 10.3969/j.issn.1001-5256.2017.12.039
[14]	Yang Liu, Zhu XiLing, Li Gong. Expression of programmed death-ligand 1 in hepatocellular carcinoma and its clinical significance[J]. Journal of Clinical Hepatology, 2016, 32(8): 1630-1634. doi: 10.3969/j.issn.1001-5256.2016.08.047
[15]	Zhang LiangJie, Chen HuiJuan, Zhao ShouSong. Effects of artificial liver plasma exchange on cytokines in patients with liver failure[J]. Journal of Clinical Hepatology, 2015, 31(4): 578-581. doi: 10.3969/j.issn.1001-5256.2015.04.024
[16]	Mao YiJia, Mao XiaoRong, Jiang Ni. Recent research on association between gene polymorphisms of IL-28B and other cytokines and HCV infection[J]. Journal of Clinical Hepatology, 2015, 31(8): 1347-1350. doi: 10.3969/j.issn.1001-5256.2015.08.044
[17]	Ma DeNing, Zhu XiaoDong, Tang ZhaoYou. Role of cancer- associated fibroblasts in growth and metastasis of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2014, 30(9): 865-868. doi: 10.3969/j.issn.1001-5256.2014.09.007
[18]	Zhang Ye, Zhang MingJie, Jia ZhanSheng. Research advances in cellular immunotherapy for primary hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2014, 30(9): 860-864. doi: 10.3969/j.issn.1001-5256.2014.09.006
[19]	Zhou Jian, Wan Hong. Relationship between changes in serum cytokine levels after artificial liver therapy and clinical prognosis in patients with liver failure[J]. Journal of Clinical Hepatology, 2013, 29(7): 535-537. doi: 1001-5256 (2013) 07-0535-03
[20]	Guan HaiTao, Zhao JinLi, Tang Rui, Sun LinLin. IL-17 promotes expression of fibrosis-related factors in hepatic stellate cells[J]. Journal of Clinical Hepatology, 2013, 29(5): 370-374.

Supplements(0)

Cited By

Cited by

Periodical cited type(10)

1.	李晏杰，王娜，王沙，唐鲲，毛德文，石清兰，龙富立. HBV相关性肝衰竭发病中的Th细胞免疫调控机制及中医药干预的研究进展. 内蒙古中医药. 2023(11): 134-138 .
2.	霍瑞静，张翠丽，李静，何晨熙，李帅，高健. NF-κB信号通路对慢性乙型肝炎患者Th17/Treg相关细胞因子水平变化的影响. 中华医院感染学杂志. 2022(17): 2570-2573 .
3.	黄杏，刘文志，陈斌. 清温并用法治疗肝衰竭的中医理论及作用机制探讨. 中国医学创新. 2021(10): 164-167 .
4.	陈斌，丁秀丽，彭杰，黎运芳，王若宇，周意. 清温并用法对慢加急性肝衰竭肠源性内毒素血症大鼠结肠组织FoxP3, ROR-γt表达的影响. 中国实验方剂学杂志. 2020(02): 19-25 .
5.	谭年花，陈斌，彭杰，朱文芳，张涛，周意，王若宇. 乙肝相关性慢加急性肝衰竭“阳黄-阴阳黄-阴黄”不同证候分型患者Treg/Th17的表达. 中国实验方剂学杂志. 2020(02): 33-38 .
6.	陈斌，黎运芳，张涛，朱文芳，彭杰，张茜茜. 清温并用法对慢加急性肝衰竭大鼠肝组织Treg/Th17型细胞因子及转录因子的影响. 中华中医药杂志. 2020(09): 4618-4622 .
7.	李薇，于佳卉，王新婷，王春梅，李贺，陈建光，孙靖辉. 北五味子总木脂素对环磷酰胺诱导的免疫功能低下小鼠的调节作用. 吉林大学学报(医学版). 2019(02): 294-299+472 .
8.	陈斌，彭杰，张涛，朱文芳，张茜茜，丁秀丽，黎运芳. 清温并用法对慢加急性肝衰竭大鼠结肠组织调节性T淋巴细胞/辅助性T淋巴细胞17细胞因子的影响. 湖南中医药大学学报. 2019(05): 573-577 .
9.	张龙跃，张序瑞. 赤芍解毒汤保留灌肠治疗慢加急性肝衰竭临床研究. 系统医学. 2019(13): 138-140 .
10.	梁琼，赵隽，苏静，梁琳，张吉发. 凉血化瘀解毒法对急性肝衰竭模型大鼠免疫功能、Toll样受体及VEGF表达的影响. 中国中医急症. 2019(10): 1700-1704 .

Other cited types(5)

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views (2894) PDF downloads(518)

Relationship between percentages of T lymphocytes in peripheral blood and deficiency of vital qi in patients with HBV- ACLF

DOI: 10.3969/j.issn.1001-5256.2014.04.009