Establishment and application value of a monosodium glutamate-rat-liver regeneration model

Li HanMin

doi:10.3969/j.issn.1001-5256.2019.11.047

Volume 35 Issue 11

Nov. 2019

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Article Navigation > Journal of Clinical Hepatology > 2019 > 35(11): 2600-2604

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Establishment and application value of a monosodium glutamate-rat-liver regeneration model

DOI: 10.3969/j.issn.1001-5256.2019.11.047

Li HanMin

Institute of Hepatology,Hubei Provincial Hospital of Traditional Chinese Medicine & Li Hanmin's Inheritance Studio for Experts of Traditional Chinese Medicine
Institute of Basic Theory of Traditional Chinese Medicine,Hubei Academy of Chinese Medicine

Research funding:

Received Date: 2019-05-30
Published Date: 2019-11-20

Abstract

Abstract

The establishment of an animal model of liver regeneration in a state of neuro-endocrine-immune network dysfunction is an urgent need to promote the research on the regulatory mechanism of liver regeneration. The monosodium glutamate( MSG)-rat-liver regeneration model is a composite model of liver regeneration which combines the MSG-rat model with the model of partial hepatectomy( PHx) or hepatotoxicity( for example,CCl4),and it is mainly used to investigate liver regeneration and its regulatory mechanism in the state of neuro-endocrine-immune network dysfunction,mechanisms and drug screening of liver-brain syndrome,and mechanisms of disease-syndrome combination and drug efficacy. Dysregulated liver regeneration process is observed in the MSG-rat-liver regeneration model established by the MSG-rat model and PHx,which is significantly different from the liver regeneration process in normal rats after major hepatectomy. The MSG-rat-liver regeneration model established by the MSG-rat model and hepatotoxicity has a significantly higher degree of liver fibrosis than normal rats with CCl4-induced liver fibrosis. The hypothalamic-pituitary-hepatic axis is an important mechanism of the influence of the external environment on liver regeneration,and the imbalance of epithelial-mesenchymal transition/mesenchymal-epithelial transition is an important mechanism of the influence of intrahepatic microenvironment on abnormal liver regeneration( liver fibrosis).The research on MSG-rat-liver regeneration model preliminarily reveals the efficacy and mechanism of traditional Chinese medicine in the prevention and treatment of liver and related diseases( including liver-brain syndrome) by influencing the neuro-endocrine-immune-liver regeneration regulatory network and promotes the research on the combination of diseases and syndromes. The establishment and application of the MSG-rat-liver regeneration model has important scientific significance and application value in further exploration of the complex mechanism of liver regeneration and the mechanism of traditional Chinese medicine in regulating liver regeneration.
- liver regeneration,
- models,animal,
- sodium glutamate

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References(22)

References

[1] LI HM. The basis and clinic of TCM regulating liver regeneration[M]. Wuhan:Huazhong University of Science and Technology Press,2016:73-77.(in Chinese)李瀚旻．中医药调控肝再生基础与临床[M]．武汉:华中科技大学出版社，2016:73-77.

[2] SHEN GJ,JIANG XY,HUANG J,et al. Dynamic changes in drug-resistant duck hepatitis B virus DNA in single hepatocyte nucleus after hemihepatectomy using a liver regeneration model[J]. J Clin Hepatol,2017,33(11):2127-2131.(in Chinese)沈国俊，江小云，黄洁，等．利用半肝切除再生模型研究单个肝细胞核内耐药鸭乙型肝炎病毒DNA的动态变化[J]．临床肝胆病杂志，2017,33(11):2127-2131.

[3] HIGGINS G,ANDERSON R. Experimental pathology of the liver. I. Restoration of the liver of the white rat following partial surgical removal[J]. Arch Pathol,1931,12(3):186-202.

[4] LI HM. Neuroendocrine-immune-liver regeneration regulatory network[J]. Chin J Integr Tradit West Med Liver Dis,2014,24(4):193-196.(in Chinese)李瀚旻．神经-内分泌-免疫-肝再生调控网络[J]．中西医结合肝病杂志，2014,24(4):193-196.

[5] LIU YF,CAI DF. Effects of monosodium levoglutamate on neuroendocrine and immune system[J]. Foreign Med Sci:Endocrinol,1997,17(3):143-145.(in Chinese)刘彦芳，蔡定芳．左旋谷氨酸单钠对神经内分泌免疫系统的影响[J]．国外医学:内分泌学分册，1997,17(3):143-145.

[6] LI HM,ZHANG LT,MEI JJ,et al. Establishment of a rat model of monosodium levoglutamate-liver regeneration[J]. World Chin J Dig,2000,8(7):824-826.(in Chinese)李瀚旻，张六通，梅家俊，等．左旋谷氨酸单钠-肝再生-大鼠模型的建立[J]．世界华人消化杂志，2000,8(7):824-826.

[7] LI HM. Establish the model of rat of deficiency of the kidneyessence and the liver-blood[J]. Chin J Basic Med Tradit Chin Med,2001,7(4):51-53.(in Chinese)李瀚旻．“肝肾精血亏虚”大鼠模型的建立[J]．中国中医基础医学杂志，2001,7(4):51-53.

[8] LI HM. The basis and clinic of TCM regulating liver regeneration[M]. Wuhan:Huazhong University of Science and Technology Press,2016:207-218.(in Chinese)李瀚旻．中医药调控肝再生基础与临床[M]．武汉:华中科技大学出版社，2016:207-218.

[9] LI HM,YANG ML,MEI JJ,et al. The effects of Zuogui Pill on expression of TGF-(alpha),(beta)and it’s receptor in ARN and regenerative liver of the MSG-regeneration-rat[J].Chin J Hepatol,2004,12(5):307-308.(in Chinese)李瀚旻，杨木兰，梅家俊，等．左归丸对大鼠转化生长因子-α、β及其受体表达的影响[J]．中华肝脏病杂志，2004,12(5):307-308.

[10] LI HM,YANG ML,MEI JJ,et al. Apoptosis of ARN neural cell in MSG-regeneration-rat and genetic expression of apoptotic related gene TGF-(beta)1[J]. Chin J Appl Physiol,2003,19(1):46-47,93.(in Chinese)李瀚旻，杨木兰，梅家俊，等．MSG-肝再生-大鼠下丘脑神经细胞凋亡及相关基因TGF-β1的表达[J]．中国应用生理学杂志，2003,19(1):46-47,93.

[11] YANG ML,LI HM,MEI JJ,et al. The expression of TGF-β1mRNA in the hypothalamic arn of rat with MSG-liver regeneration using in situ hybridization[J]. Chin J Histochem Cytochem,2002,11(2):202-204.(in Chinese)杨木兰，李瀚旻，梅家俊，等．Dig标记探针原位杂交检测MSG-肝再生-大鼠下丘脑弓状核TGF-β1mRNA[J]．中国组织化学与细胞化学杂志，2002,11(2):202-204.

[12] LI HM,GAO X,YANG ML,et al. Effects of Zuogui Wan on neurocyte apoptosis and down-regulation TGF-β1 expression in nuclei of arcuate hypothalamus of monosodium glutamate—liver regeneration rats[J]. World J Gastroenterol,2004,10(19):2823-2826.

[13] LI HM,GAO X,ZHOU MS. Gene expression profile of liver regeneration induced by monosodium L-glutamate in rat[J].World Chin J Dig,2005,13(4):448-451.(in Chinese)李瀚旻，高翔，周密思．MSG-肝再生-大鼠再生肝组织基因表达谱分析[J]．世界华人消化杂志，2005,13(4):448-451.

[14] LI HM. The basis and clinic of TCM regulating liver regeneration[M]. Wuhan:Huazhong University of Science and Technology Press,2016:315-327.(in Chinese)李瀚旻．中医药调控肝再生基础与临床[M]．武汉:华中科技大学出版社，2016:315-327.

[15] LI HM,ZHANG LT,QIU XF,et al. Study of the effect and the mechanism of Zougui Pill on the deficiency of kidney-essence and liver-blood of model of MSG-regeneration-rat[J].Med J Wuhan Univ,2001,3(4):30-33.(in Chinese)李瀚旻，张六通，邱幸凡，等．左归丸改善MSG-肝再生-大鼠肝肾精血亏虚证的作用机理研究[J]．湖北中医学院学报，2001,3(4):30-33.

[16] SONG HL,LI HM,LIN LS,et al. Effects of Diwuyanggan capsule on liver regeneration of rat with deficiency of kidney essence and liver blood[J]. Chin J Integr Tradit West Med Liver Dis,2013,23(2):90-92.(in Chinese)宋红丽，李瀚旻，林立生，等．地五养肝胶囊对肝肾精虚大鼠肝再生的影响[J]．中西医结合肝病杂志，2013,23(2):90-92.

[17] LI HM,GAO X,ZHOU MS. Effects of Zuogui pill on gene expression profile of regenerate hepatic tissue in MSG-liver regeneration-rat[J]. China J Tradit Chin Med Pharm,2006,21(2):104-106.(in Chinese)李瀚旻，高翔，周密思．左归丸对MSG-肝再生-大鼠再生肝组织基因表达谱的影响[J]．中华中医药杂志，2006,21(2):104-106.

[18] LI HM,GAO X,ZHOU MS. Zuogui pill regulating gene expression profile in liver of MSG-induced liver regeneration in rats[J]. Chin J Basic Med Tradit Chin Med,2005,11(8):595-598.(in Chinese)李瀚旻，高翔，周密思．左归丸针对性调节MSG-肝再生-大鼠再生肝组织基因表达[J]．中国中医基础医学杂志，2005,11(8):595-598.

[19] ZHAO BB,LONG QH,WANG P,et al. Protective effect of Chaihu Shugan San on liver injury and neurotrophic factors in brain area of depression in MSG-rat-liver regeneration model[J]. Lishizhen Med Mater Med Res,2018,29(12):2833-2837.(in Chinese)赵宾宾，龙清华，王平，等．柴胡疏肝散对MSG-大鼠-肝再生模型肝脏损伤和抑郁症脑区神经营养因子的保护作用[J]．时珍国医国药，2018,29(12):2833-2837.

[20] ZHAO BB,LONG QH,WANG CY,et al. Protective effects of Liu Wei Di Huang Wan on the liver,orbitofrontal cortex nissl bodies,and neurites in MSG+PH-induced liver regeneration rat msodel[J]. Evid Based Complement Alternat Med,2018,2018:9090128.

[21] LI HM. Prevention and treatment of liver fibrosis by regulating liver regeneration[J]. J Clin Hepatol,2015,31(6):992-995.(in Chinese)李瀚旻．从调控肝再生探讨肝纤维化的防治[J]．临床肝胆病杂志，2015,31(6):992-995.

[22] LIU CH,ZHAO ZM,LYU J. Advances in the understanding and treatment of liver fibrosis in traditional Chinese medicine[J]. J Clin Hepatol,2019,35(4):728-733.(in Chinese)刘成海，赵志敏，吕靖．中医对肝纤维化逆转的认识与治疗[J]．临床肝胆病杂志，2019,35(4):728-733.

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Establishment and application value of a monosodium glutamate-rat-liver regeneration model

DOI: 10.3969/j.issn.1001-5256.2019.11.047