Objective To investigate the effect of CD4+CD25+ regulatory T cells (Treg) in the proliferation of hepatic stellate cells (HSC) and the secretion of Hyaluronic Acid (HA) .Methods By using magnetic activated cell sorter (MRCS) , Treg cells were isolated from peripheral blood of chronic hepatitis B patients The hepatic stellate cells (HSC) cultured alone were used as blank group.The LX-2 were co-cultured with Treg cells in vitro as co-culture group;adding TGFβ1 to HSC co-cultured as control group.CCK-8 method for the detection of the proliferation of HSC, ELISA analysis for the detection of HA secretion was performed.One-way analysis of variance was used for the comparison.Results Compared with the blank group (0.6623±0.02702) the proliferation of HSC of the co-culture group was significantly higherand the most prominent effect was 2.5∶ 1 (0.6836±0.00560) , while the proliferation of HSC decreased at 1∶ 1、5∶ 1 and 7.5∶ 1, and the discrepancy between co-culture subgroups and blank group had statistical significance (F=11.421, 5.272, 84.514, 490.639;P<0.05) .Compared with blank group, the proliferation of HSCs of different concentrations of TGFβ1 control subgroup increased significantly (0.6720±0.00753, 0.6773±0.00585, 0.5373±0.00387) and reached a peak at 8 ng/μl, and the discrepancy between control subgroups and blank group had statistical significance (F=196.175, 799.624, 236.461;P<0.01) .Linear correlation analysis showed concentrations of HA and the proliferation of HSC in co-culture group was positively correlated with control group (r=0.661, 0.871;P<0.01) .Conclusion CD4+CD25+ Treg cells could accelerate the cultivation of HSCs proliferation and HA secretion.And, in vitro, we confirmed CD4+CD25+ Treg cells play an important role in the process of chronic hepatitis B.
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