ObjectiveTo investigate the distribution characteristics of hepatitis C virus (HCV) genotypes in patients with hepatitis C in Guizhou, China, and to provide a basis for the prevention and individualized treatment of HCV infection. MethodsA total of 1211 HCV RNA-positive patients with hepatitis C who were treated in Guiyang Public Health Clinical Center from September 2011 to October 2018 were enrolled. PCR direct sequencing was performed to obtain HCV sequences, which were then compared with the known HCV sequences in GenBank to obtain HCV genotypes and subgenotypes. The association of genotype distribution with sex, age, ethnic group, region, and route of infection was analyzed. The chi-square test or the Fisher’s exact test was used for comparison of categorical data between two groups. ResultsA total of 4 genotypes and 11 subgenotypes were detected among the 1211 individuals with HCV infection, with the main genotypes of 1b (26.84%), 3b (27.17%), and 6a (24.28%). There was a significant difference in the distribution of HCV genotypes between the male and female individuals (χ2=15.428, P=0.009); 29.34% of the male individuals had genotype 3b, and 32.21% of the female individuals had genotype 1b. There was a significant difference in the distribution of HCV genotypes between different age groups (χ2=67.439, P<0.001); genotype 1b was the main genotype in the individuals aged ≤18 years (66.67%) or ≥60 years (58.93%), genotypes 3b and 6 were the main genotypes in the individuals aged 19-39 years (28.93% and 29.29%, respectively), and genotypes 1b, 3b, and 6 were the main genotypes in the individuals aged 40-59 years (29.54%, 27.33%, and 24.28%, respectively). There was a significant difference in the distribution of HCV genotypes between the individuals with different routes of infection (χ2=153.916, P<0.001); the most common route of infection was intravenous drug addiction (57.97%), followed by sexual contact (8.42%) and invasive cosmetic surgery (8.42%); genotype 3b was the main genotype in the individuals with intravenous drug addiction (31.48%) or invasive cosmetic surgery (32.35%), and genotype 6 was the main genotype in the individuals with sexual contact (36.27%). There was no significant difference in the distribution of HCV genotypes between the individuals in different ethnic groups or from different regions of Guizhou (both P>0.05). ConclusionThe distribution of HCV genotypes is diverse in Guizhou, and HCV strains with genotypes 3b, 1b, and 6a are the main epidemic strains. Several rare subgenotypes of HCV genotype 6 are observed. There is a significant difference in the distribution of HCV genotypes between the individuals with different ages, sexes, or routes of infection.
[1] SARRAZIN C,ZEUZEM S. Resistance to direct antiviral agents in patients with hepatitis C virus infection[J]. Gastroenterology,2010,138(2):447-462.
|
[2] SIMMONDS P,BUKH J,COMBET C,et al. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes[J]. Hepatology,2005,42(4):962-973.
|
[3] SMITH DB,BUKH J,KUIKEN C,et al. Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes:Updated criteria and genotype assignment web resource[J].Hepatology,2014,59(1):318-327.
|
[4] Chinese Society of Hepatology and Chinese Society of Infectious Diseases,Chinese Medical Association. The guideline of prevention and treatment for hepatitis C:A 2015 update[J]. J Clin Hepatol,2015,31(12):1961-1979.(in Chinese)中华医学会肝病学分会,中华医学会感染病学分会.丙型肝炎防治指南(2015年更新版)[J].临床肝胆病杂志,2015,31(12):1961-1979.
|
[5] Polaris Observatory HCV Collaborators. Global prevalence and genotype distribution of hepatitis C virus infection in 2015:A modelling study[J]. Lancet Gastroenterol Hepatol,2017,2(3):161-176.
|
[6] MESSINA JP,HUMPHREYS I,FLAXMAN A,et al. Global distribution and prevalence of hepatitis C virus genotypes[J].Hepatology,2015,61(1):77-87.
|
[7] BORGIA SM,HEDSKOG C,PARHY B,et al. Identification of a novel hepatitis C virus genotype from Punjab,India:Expanding classification of hepatitis C virus into 8 genotypes[J]. J Infect Dis,2018,218(11):1722-1729.
|
[8] MURPHY DG,SABLON E,CHAMBERLAND J,et al. Hepatitis C virus genotype 7,a new genotype originating from central Africa[J]. J Clin Microbiol,2015,53(3):967-972.
|
[9] SU YY,LIU HX,WANG N. Hepatitis C virus genotypes in China:A systematic review[J]. Chin J Epidemiol,2013,34(1):80-84.(in Chinese)苏迎盈,刘慧鑫,汪宁.中国丙型肝炎病毒基因型分布[J].中华流行病学杂志,2013,34(1):80-84.
|
[10] HUANG CQ,WANG FX. Genotype of HCV genes in a tertiary hospital in Harbin[J/CD]. Chin J Exp Clin Infect Dis(Electronic Edition),2017,11(5):483-489.(in Chinese)黄超群,王福祥.哈尔滨某三甲医院丙型肝炎病毒基因分型研究[J/CD].中华实验和临床感染病杂志(电子版),2017,11(5):483-489.
|
[11] LIN J,WEN XY,TANG M. Hepatitis C viral genotyping and its relation with clinic among Han population in southern Sichuan area[J]. Lab Med Clin,2016,13(10):1359-1361.(in Chinese)林江,温先勇,唐敏.川南地区汉族人群丙型肝炎病毒基因分型及其与临床关系[J].检验医学与临床,2016,13(10):1359-1361.
|
[12] ZHOU ZG,ZENG T,XU H,et al. Genotype distribution and clinical significance of hepatitis C virus in Chongqing[J]. J Chongqing Med Univ,2015,40(3):383-387.(in Chinese)周召刚,曾藤,徐华,等.重庆地区丙型肝炎病毒基因型分布及临床意义[J].重庆医科大学学报,2015,40(3):383-387.
|
[13] DING JJ,LI YY,TIAN M. Analysis of genotypes of HCV in Guizhou by line probe nucleic acid hybeidization[J]. Chin J Exp Clin Virol,1999,13(3):243-246.(in Chinese)丁静娟,李媛媛,田苗.线探针核酸杂交分析贵州地区HCV感染株基因型[J].中华实验和病毒学杂志,1999,13(3):243-246.
|
[14] YANG XL,LIANG YD,LI YF,et al. Study on hepatitis C virus genotyping in Guizhou area[J]. Chongqing Med,2014,43(30):4065-4067,4070.(in Chinese)杨兴林,梁跃东,李勇富,等.贵州地区丙型肝炎病毒基因分型的研究[J].重庆医学,2014,43(30):4065-4067,4070.
|
[15] LI J,CHEN J,ZHUANG H. Epidemiology of hepatitis C[J]. J Prac Hepatol,2012,15(5):379-381.(in Chinese)李杰,陈杰,庄辉.丙型肝炎的流行病学[J].实用肝脏病杂志,2012,15(5):379-381.
|
[16] FU Y,QIN W,CAO H,et al. HCV 6a prevalence in Guangdong province had the origin from Vietnam and recent dissemination to other regions of China:Phylogeographic analyses[J]. PLo S One,2012,7(1):e28006.
|
[17] ZHOU DX,TANG JW,CHU IM,et al. Hepatitis C virus genotype distribution among intravenous drug user and the general population in Hong Kong[J]. J Med Virol,2006,78(5):574-581.
|
[18] WANG Y,WU HY,ZHAO XC,et al. Evolutionary characteristics and positive selection site of hepatitis C virus isolated in intravenous drug users in Pudong new district,Shanghai[J].Chin J Epidemiol,2017,38(3):378-383.(in Chinese)王郁,吴红岩,赵希畅,等.上海市浦东新区注射吸毒者HCV的进化分析与正选择位点研究[J].中华流行病学杂志,2017,38(3):378-383.
|
[19] TANG W,SU MH,JIANG JN,et al. Epidemiological characteristics and genotype distribution of hepatitis C virus in Guangxi[J]. World Chin J Dig,2014,22(9):1300-1306.(in Chinese)唐维,苏明华,江建宁,等.广西地区丙型肝炎病毒的基因型分布与流行病学特征[J].世界华人消化杂志,2014,22(9):1300-1306.
|
[20] YAN Z,FAN K,WANG Y,et al. Changing pattern of clinical epidemiology on hepatitis C virus infection in southwest China[J]. Hepat Mon,2012,12(3):196-204.
|
[21] PENG XB,MAO XR,CHEN H. Genotypes of HCV in hepatitis C patients of Han nationality in Gansu,China[J]. J Clin Hepatol,2013,29(11):828-831.(in Chinese)彭雪彬,毛小荣,陈红.甘肃地区汉族HCV基因分型相关分析[J].临床肝胆病杂志,2013,29(11):828-831.
|
[22] LI W,KANG Y,DING GQ,et al. Clinical effect and safety of sofosbuvir-ledipasvir regimen in treatment of patients with HCV genotype 6a chronic hepatitis C[J]. J Clin Hepatol,2019,35(3):526-529.(in Chinese)李威,康谊,丁岗强,等.sofosbuvir/ledipasvir方案治疗HCV6a型慢性丙型肝炎的临床效果和安全性[J].临床肝胆病杂志,2019,35(3):526-529.
|
[23] YANG WW. Influencing factor on the efficacy of combined treatment of pegylated interferon-αand ribavirin in patients with chronic hepatitis C[J/CD]. Chin J Exp Clin Infect Dis(Electronic Edition),2018,12(3):301-306.(in Chinese)杨炜炜.聚乙二醇化干扰素联合利巴韦林治疗慢性丙型肝炎疗效的影响因素[J/CD].中华实验和临床感染病杂志(电子版),2018,12(3):301-306.
|
[1] | Yuanzhi HUANG, Fuchuan WANG, Yi DONG, Zhiqiang XU, Yinjie GAO, Jianguo YAN, Lili CAO, Danni FENG, Min ZHANG. Liver disease phenotypes and clinical features of patients with different genotypes of Wilson’s disease[J]. Journal of Clinical Hepatology, 2024, 40(8): 1627-1632. doi: 10.12449/JCH240819 |
[2] | Jie ZHOU, Jinmao LIAO, Ling LIAO, Huanchun YANG, Zhan LIU. A rare ATP7B genotype identified in the siblings with hepatolenticular degeneration and their pedigree analysis[J]. Journal of Clinical Hepatology, 2022, 38(5): 1122-1125. doi: 10.3969/j.issn.1001-5256.2022.05.029 |
[3] | Qiu Chen, Jia ShengNan, Zhang Qian, Zhang ChuanHui, Jin ZhenJing, Yang LanLan. Patients with mixed genotype hepatitis C virus infection treated by sofosbuvir/velpatasvir/ribavirin: A case report[J]. Journal of Clinical Hepatology, 2020, 36(7): 1591-1592. doi: 10.3969/j.issn.1001-5256.2020.07.028 |
[4] | Wang Mei, Xiong HuaGang, Yang ZhiGang, Long Hai, Wang Yi, Tan Li. HCV genotype and clinical features of patients with HIV/HCV coinfection in Guizhou,China: An analysis of 383 cases[J]. Journal of Clinical Hepatology, 2020, 36(10): 2203-2208. doi: 10.3969/j.issn.1001-5256.2020.10.009 |
[6] | Ding ShiMei, Qu Wei, Liang Xi, Jiao Yang, Zhang Jing, Jia XiaoLi, Wang WenJun, Dang ShuangSuo. Relationship between chronic hepatitis C virus genotype and thyroid function[J]. Journal of Clinical Hepatology, 2015, 31(8): 1271-1274. doi: 10.3969/j.issn.1001-5256.2015.08.021 |
[7] | Luo Shan, Chen Fei, Zhang Jian, Wu ZhiHui, Ou WenSheng, Huang Jian, Wen Bo, Ju XiaoWang. Hepatitis C virus infection and genotype distribution among injecting drug users in methadone clinics in Chenzhou, China[J]. Journal of Clinical Hepatology, 2015, 31(11): 1841-1844. doi: 10.3969/j.issn.1001-5256.2015.11.017 |
[8] | Zhang YanFang, Zhao DongMei, Zhang YanMin, Wang Wei, Zhao HongRu. An analysis of related factors for hepatitis C genotyping and sustained virologic response in Xingtai, China[J]. Journal of Clinical Hepatology, 2015, 31(11): 1845-1848. doi: 10.3969/j.issn.1001-5256.2015.11.018 |
[9] | Gu Bin, Huang HuiQin, Hou XiaoLan, Ning Feng, Zhang Yan, Li Lu. Relationship between hepatitis C virus genotypes and viral load in Chenzhou, China[J]. Journal of Clinical Hepatology, 2013, 29(11): 832-834. doi: 10.3969/j.issn.1001-5256.2013.11.008 |
[10] | Deng Le, Wen ZhiLi. Review of recent research progress in correlating HBV genotypes with efficacy of antiviral treatments[J]. Journal of Clinical Hepatology, 2012, 28(6): 469-473. |
[11] | Guo XinHui, Yan Yan, Li Zhuo, Yin JiMing. Fluorescent reverse transcription-PCR assay for determination of hepatitis C virus genotypes[J]. Journal of Clinical Hepatology, 2011, 27(1): 65-67. |
[12] | Cheng Ling, Hong GuoHu, Guo Yan, Mao Qing. Prediction of HLA-DRB1*0311/0401 in recognizing epitopes of HCV Core protein of different genotypes[J]. Journal of Clinical Hepatology, 2011, 27(1): 61-64. |
[13] | Zhang WenJie, Liu LiJun, Du ShaoCai, Li ChaoXia, Lu QingYun, Yang ShaoHua. The prevalence of hepatitis C virus genotype in Lanzhou area[J]. Journal of Clinical Hepatology, 2010, 26(3): 290-291+294. |
[15] | Chen Yan, Tian DeYing, Xia NingShao. Epidemiology and the genotypes of HEV isolated from patients with hepatitis E in Wuhan[J]. Journal of Clinical Hepatology, 2006, 22(1): 36-38. |
[16] | Xu ZhengJu, Yang Hong, Zhang QiHua, Chen XianLi, Li ShuQing, Wang ChongGuo. Study on the relationship between hepatitis B virus genotypes and effect of lamivudine therapy.[J]. Journal of Clinical Hepatology, 2005, 21(3): 157-159. |
[18] | Liu ChengYong, Hou YuanPei, Qian XiuMei, Sun Qing, Song Li. Distribution of hepatitis B virus genotype in Xuzhou and its clinical significance[J]. Journal of Clinical Hepatology, 2005, 21(3): 151-152. |
[19] | Li TingMing, Zhu YouFu, He HaiTang, Mao QianGuo, Wu AiHua, Liang YuFang, Luo KangXian. The relation between chinese hepatitis B virus genotype B C and efficacy of interferon-α.[J]. Journal of Clinical Hepatology, 2005, 21(1): 16-18. |
[20] | Jiao Jian, Wang JiangBin. Distribution of HCV genotypes and its clinical features in patients coinfected with HCV and HBV[J]. Journal of Clinical Hepatology, 2004, 20(2): 75-76. |
1. | 崔蕊,马杈,杨天芬,周浩军,李萍萍. 直接抗病毒药物治疗失败的慢性丙型肝炎患者临床特点及基因型分析. 肝脏. 2024(08): 943-946 . ![]() | |
2. | 冯程,陈友惠,李黎,王辉,李生浩,杨永锐. HCV合并HIV和HBV患者的实验室指标变化特征分析. 云南医药. 2024(06): 13-17 . ![]() | |
3. | 刘宏,官越,兰发强,陈晨. 泸州地区901例HCV感染者基因型分布特征. 热带医学杂志. 2024(12): 1773-1776 . ![]() | |
4. | 木朝宇,张晓梅,范德胜,刘培明,姚慧琳. 淮北市HCV感染者基因亚型分布特征及1b型天然耐药突变分析. 蚌埠医学院学报. 2023(02): 260-263 . ![]() | |
5. | 宫瑞,蒋红梅,孙广,于明瑞,岳龙飞,张云东. 502例丙型肝炎患者病毒基因分型及与性别和年龄的关系研究. 现代生物医学进展. 2021(06): 1147-1150 . ![]() | |
6. | 杜本丽,杨萱,官平珍,颜芬,屈生毕,唐仁海,王译葵,王继宝,杨跃诚,叶润华,姚仕堂,丁盈盈,段松. 云南省芒市美沙酮维持治疗门诊服药者HCV感染及治疗现状分析. 中国艾滋病性病. 2021(08): 877-881 . ![]() | |
7. | 陈友惠,高建鹏,王辉,冯程,李俊义,母昌垒,李婉澜,许艳玲,李畏娴,李黎. 昆明地区丙型肝炎病毒基因型分布特征的研究. 现代医学. 2021(07): 747-750 . ![]() | |
8. | 杨自力,张洁,陈娟娟,任皓月,万祎. HCV基因型、AFP与P53抗体、ANA在HCV相关肝细胞癌中的相关性. 热带医学杂志. 2021(09): 1134-1138 . ![]() | |
9. | 王梅,熊华刚,杨智刚,龙海,王艺,谭丽. 贵州省383例HIV/HCV共感染者HCV基因分型及临床特征. 临床肝胆病杂志. 2020(10): 2203-2208 . ![]() |