Expression and significance of OX40/OX40L in peripheral blood of patients with autoimmune hepatitis,primary biliary cholangitis,and their overlap syndrome

WANG WeiZhao; ZHU QinLing; XIANG XiaoXing; MA Li; GU Da; TONG Cong; WANG TingTing; HE JiaJun; LIU Jun; WANG Lu; CHEN ChaoWu

doi:10.3969/j.issn.1001-5256.2020.12.020

Volume 36 Issue 12

Dec. 2020

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Article Navigation > Journal of Clinical Hepatology > 2020 > 36(12): 2740-2745

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Expression and significance of OX40/OX40L in peripheral blood of patients with autoimmune hepatitis,primary biliary cholangitis,and their overlap syndrome

DOI: 10.3969/j.issn.1001-5256.2020.12.020

Center of Endoscopic Diagnosis and Treatment,Subei People's Hospital of Jiangsu & Clinical Medical College of Yangzhou University
Department of Gastroenterology,Subei People's Hospital of Jiangsu & Clinical Medical College of Yangzhou University
Graduate School,Dalian Medical University
Center of Rheumatology and Immunology,Subei People's Hospital of Jiangsu & Clinical Medical College of Yangzhou University

Received Date: 2020-06-10
Published Date: 2020-12-20

Abstract

Abstract

Objective To investigate the expression and clinical significance of OX40/OX40 L( CD134/CD134 L) in CD4 + T cells,CD8 +T cells,monocytes,and B lymphocytes in peripheral blood of patients with autoimmune hepatitis( AIH),primary biliary cholangitis( PBC),and their overlap syndrome before and after standardized treatment. Methods A total of 74 patients with AIH,PBC,and their o-verlap syndrome who were diagnosed in Subei People's Hospital of Jiangsu from August 2015 to August 2019 were enrolled,and according to related diagnostic criteria,they were divided into AIH group( group A) with 29 patients,PBC group( group P) with 26 patients,and overlap syndrome group( group C) with 19 patients. A healthy control group with 30 individuals was also established. Peripheral blood samples were collected before and after standardized treatment to measure the expression of OX40/OX40 L on the surface of peripheral blood cells by immunofluorescence flow cytometry,and the expression of OX40/OX40 L was compared before and after treatment and between the three groups and the healthy control group to investigate its clinical significance. A one-way analysis of variance was used for comparison between multiple groups,and the least significant difference t-test was used for further comparison between two groups; the paired t-test was used for comparison of paired samples between two groups. Results There were no significant differences in sex composition and age composition between the three groups( P > 0. 05). Before treatment,the positive rate of OX40 in peripheral blood CD4+T cells gradually increased in groups A,P,and C,and groups A,P,and C had a significantly higher positive rate of OX40 than the control group( 14. 80% ± 4. 99%/17. 11% ± 2. 71%/25. 18% ± 5. 55% vs 6. 67% ± 2. 26%,F = 14. 823,P < 0. 001); groups A,P,and C had a significantly higher positive rate of OX40 in CD8+T cells than the control group( 4. 86% ± 1. 54%/6. 40% ± 1. 88%/7. 33% ± 2. 12% vs 4. 09% ± 2. 69%,F =5. 486,P < 0. 001); the positive rate of OX40 L in CD14+monocytes was 19. 84% ± 6. 11% in group A,21. 17% ± 4. 35% in group P,29. 13% ± 6. 32% in group C,and 4. 86% ± 2. 34% in the control group,and there was a significant difference between groups( F =17. 004,P < 0. 001); the positive rate of OX40 L in CD19+B cells was 17. 62% ± 3. 86% in group A,14. 75% ± 4. 32% in group P,10. 13% ± 2. 56% in group C,and 4. 50% ± 1. 38% in the control group,showing a trend of gradual reduction,and groups A,P,and C had a significantly higher positive rate than the control group( F = 12. 221,P < 0. 001). After treatment,the positive rate of OX40 in CD8+T cells decreased significantly to a similar level as the control group,and there was no significant difference between groups( F =0. 731,P = 0. 538). For the other three types of cells,although there were varying degrees of reduction in the positive rate of OX40/OX40 L after treatment,groups A,P,and C still had a significantly higher positive rate than the control group; in CD4+T cells,the positive rate of OX40 was 11. 00% ± 1. 98% in group A,13. 72% ± 1. 03% in group P,19. 72% ± 3. 47% in group C,and 6. 67% ± 2. 26% in the control group,and groups A,P,and C had a significantly higher positive rate than the control group( F = 11. 365,P < 0. 001); in CD14+monocytes,the positive rate of OX40 L was 11. 82% ± 2. 23% in group A,15. 19% ± 4. 42% in group P,24. 51% ± 4. 09% in group C,and 4. 86% ± 2. 34% in the control group,and groups A,P,and C had a significantly higher positive rate than the control group( F =13. 748,P < 0. 001); in CD19+B cells,the positive rate of OX40 L was 9. 09% ± 3. 25% in group A,6. 81% ± 2. 20% in group P,7. 48% ± 2. 85% in group C,and 4. 50% ± 1. 38% in the control group,and groups A,P,and C had a significantly higher positive rate than the control group( F = 8. 052,P < 0. 001). Groups A,P,and C had significant reductions in the expression of OX40/OX40 L in peripheral blood CD4+T cells,CD8+T cells,CD14+monocytes,and CD19+B lymphocytes after treatment( all P < 0. 05). Conclusion The expression of OX40/OX40 L in peripheral blood increases in patients with AIH,PBC,and their overlap syndrome and decreases after treatment,indicating that the OX40/OX40 L pathway is involved in the pathogenesis of the above diseases,and the role of OX40 on the surface of CD8+T cells may better reflect the treatment outcome.
- hepatitis,autoimmune,
- primary biliary cholangitis,
- overlap syndrome,
- receptors,OX40,
- OX40 ligand

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References

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Expression and significance of OX40/OX40L in peripheral blood of patients with autoimmune hepatitis,primary biliary cholangitis,and their overlap syndrome

DOI: 10.3969/j.issn.1001-5256.2020.12.020