Association of the expression level of the long non-coding RNA TINCR in peripheral blood with Th1/Th2 balance and prognosis after transcatheter arterial chemoembolization in patients with hepatocellular carcinoma

Tao BAI; Jingli WANG; Xudong HU; Bing XIA; Hailin CHENG; Juan WANG

doi:10.3969/j.issn.1001-5256.2021.01.016

Volume 37 Issue 1

Jan. 2021

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Article Navigation > Journal of Clinical Hepatology > 2021 > 37(1): 79-83

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Association of the expression level of the long non-coding RNA TINCR in peripheral blood with Th1/Th2 balance and prognosis after transcatheter arterial chemoembolization in patients with hepatocellular carcinoma

DOI: 10.3969/j.issn.1001-5256.2021.01.016

a.
Department of Gastroenterology, Jinyintan Hospital, Wuhan 430023, China
b.
Department of Hepatology, Jinyintan Hospital, Wuhan 430023, China
c.
Department of Infectious Diseases, Jinyintan Hospital, Wuhan 430023, China

Received Date: 2020-04-30
Accepted Date: 2020-08-21
Published Date: 2021-01-20

Abstract

Abstract

Objective To investigate the association of the expression level of the long non-coding RNA (lncRNA) TINCR in peripheral blood with Th1/Th2 balance and prognosis after transcatheter arterial chemoembolization (TACE) in patients with hepatocellular carcinoma (HCC). Methods A retrospective analysis was performed for the clinical data of 85 patients with HCC who were treated with TACE in Jinyintan Hospital from March 2015 to March 2017, and 30 healthy volunteers who underwent physical examination in our hospital were randomly selected as control group. The expression of lncRNA TINCR and Th1/Th2 cytokines in peripheral blood were measured on day 7 after TACE for HCC patients and the control group. The t-test was used for comparison of continuous data, the chi-square test was used for comparison of categorical data, and the Kruskal-Wallis H test was used for comparison of ranked data. A Spearman correlation analysis was used to investigate the correlation of the relative expression of lncRNA TINCR with Th1/Th2 cytokines. The Kaplan-Meier survival curves were used to investigate the influence of the relative expression of lncRNA TINCR on survival, and the log-rank test was used for comparison of survival curves. Results The HCC group had significantly higher relative expression of lncRNA TINCR in peripheral blood than the control group (1.784±0.429 vs 0.926±0.263, t=10.277, P < 0.05). The relative expression of lncRNA TINCR was associated with vascular invasion, tumor size, tumor stage, and serum alpha-fetoprotein (t=3.958, 4.499, 3.361, and 4.949, P < 0.001, P < 0.001, P=0.001, and P < 0.001). Compared with the control group, the HCC group had significantly lower serum levels of interferon-γ (IFNγ)[(13.48±5.20) pg/ml vs (27.49±7.21) pg/ml, t=11.408, P < 0.05] and interleukin-2 (IL-2) [(21.89±6.45) pg/ml vs (32.05±7.89) pg/ml, t=6.986, P < 0.05] and significantly higher serum levels of interleukin-4 (IL-4) [(13.73±3.35) pg/ml vs (9.36±2.73) pg/ml, t=6.426, P < 0.05] and interleukin-10 (IL-10) [(12.75±2.74) pg/ml vs (8.93±2.16) pg/ml, t=6.909, P < 0.05]. In HCC patients, the relative expression of lncRNA TINCR in peripheral blood was negatively correlated with the serum levels of IFNγ and IL-2(r=-3.164 and -3.270, both P < 0.001) and was positively correlated with the serum levels of IL-4 and IL-10 (r=2.963 and 3.044, P=0.007 and P < 0.001). With the relative expression of lncRNA TINCR of ≥1.700 as high expression, there were 47 HCC patients with high expression and 38 HCC patients with low expression. The log-rank test showed that the patients with low expression of lncRNA TINCR had significantly better survival than those with high expression (χ²=4.182, P < 0.05). Conclusion HCC patients have significantly higher expression of lncRNA TINCR in peripheral blood than the healthy population, and the expression of lncRNA TINCR is closely associated with the pathological features and Th1/Th2 immune balance of patients and can thus be used as one of the potential indicators for predicting the survival and prognosis of patients.
- Carcinoma, Hepatocellular,
- Chemoembolization, Therapeutic,
- RNA, Long Noncoding,
- Th1-Th2 Balance,
- Prognosis

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References

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Association of the expression level of the long non-coding RNA TINCR in peripheral blood with Th1/Th2 balance and prognosis after transcatheter arterial chemoembolization in patients with hepatocellular carcinoma

DOI: 10.3969/j.issn.1001-5256.2021.01.016