P16 protein expression and DNA quantitative analysis in human hepatocellular carcinoma

Meng ZhiLan; Ye DaXiong; Song XiaoHua; Yang ChunMing; Zhang Ning

Issue 3

Mar. 2003

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Article Navigation > Journal of Clinical Hepatology > 2003 > 19(3): 154-155

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肝细胞癌p16蛋白的表达及DNA含量分析

Published Date: 2003-03-20

Abstract

Abstract

To investigate p16 protein expression and DNA quantitative analysis and clinical significance. p16 protein expression was quantitatively determined by immunohistochemistry in 60 cases of hepatocellular carcinoma. DNA quantitative analysis of 60 cases of hepatocellular carcinoma was performed by imagecytometry. Simultaneously 20 cases of cirrhosis adjacent tumor tissues were contrasted. p16 protein expression in 60 cases of hepatocellular carcinoma was significantly lower than that in cirrhosis adjacent tumor tissues. The expression of p16 protein becomes lower with the increase in grading. The mean of DNA relative ploid (Uvalue) in hepatocellular carcinoma was from 1.08 to 3.17. U value becomes higher with the increase in grading (P<0.05) . Abnormal expression of p16 protein is closely correlated to the occurrence, development and malignancy degree of hepatocellular carcinoma. DNA quantity in hepatocellular carcinoma is closely correlated to pathological feature and clinical biological behavior. Combining both may serve as an indicator to predict the prognosis of patients.
- Hepatocellular carcinoma,
- Protein p16,
- Immunohistochemistry,
- Image cytometry

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肝细胞癌p16蛋白的表达及DNA含量分析

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肝细胞癌p16蛋白的表达及DNA含量分析

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