中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 3
Mar.  2013

Comparative analysis of serum markers of cardiac function in a rabbit model of and human patients with obstructive jaundice 

  • Published Date: 2013-03-20
  • Objective To investigate whether the serum markers of cardiac function determined in a rabbit model with experimentally induced obstructive jaundice are similar to those in human patients with obstructive jaundice.Methods Reversible obstructive jaundice was induced in rabbits by gripping the general bile ducts with a 15 cm hemostasis pincer for 3 min.Sham-operated rabbits were used as controls.Serum samples were taken at day 30 post-surgery.Thirty patients with obstructive jaundice due to biliary stones, and 30 age-and sex-matched healthy controls were recruited for study.Fasting serum samples were collected for analysis.Changes in serum cardiac markers were assayed by the Beckman-Coulter CX-7 automatic biochemical analyzer and 81600N immunoassay system.Significance of differences between groups was assessed by Dunnett's t-test.Results In the rabbit model, total bilirubin (TBil) and direct bilirubin (DBil) were significantly elevated (vs.sham-operated control, P< 0.01) .In addition, the jaundice model showed significantly increased serum creatine kinase isoenzyme (CK-MB;8.76±2.36 vs.2.36±1.12 μg/L) , myoglobin (MYO;88.4±24.9 vs.31.3±12.4 μg/L) , and cardiac troponin-I (TROPI;0.35±0.11 vs.0.01±0.01 μg/L) (all P< 0.01) .Similarly, the patients with obstructive jaundice showed significantly higher levels of CK-MB (23.93±5.11 vs.2.06±0.82 μg/L) , MYO (188.8±36.5 vs.25.4±10.2 μg/L) , and TROPI (0.34±0.08 vs.0.01±0.01 μg/L) (all P< 0.01) .Conclusion The elevated serum levels of cardiac markers in the rabbit model of experimentally induced obstructive jaundice rabbits are resemble those in human patients with obstructive jaundice due to biliary stones.The rabbit model is an appropriate tool for investigating myocardial injury related to obstructive jaundice.

     

  • [1]Dong L, Guo JY, Huang YN, et al.The research application of endo-scopic double stents for malignant obstructive jaundice[J].J Clin Hep-atol, 2012, 28 (2) :103-104. (in Chinese) 董琳, 郭建阳, 黄允宁, 等.内镜下双支架联合治疗恶性梗阻性黄疸的探讨[J].临床肝胆病杂志, 2012, 28 (2) :103-104.
    [2]李海军, 刘颖斌, 方河清, 等.肿瘤坏死因子、内皮素-1和一氧化氮在梗阻性黄疸大鼠血浆和肝组织中活性变化及甘氨酸治疗的研究[J].中华普通外科杂志, 2003, 18 (9) :567-568.
    [3]Gong P, Wang ZY, Zhao ZW, et al.Expression of TNF-αandsuperoxide Dismutase mRNA in Myocardium of rats with obstructivejaundice[J].Chin J Bases General Surg, 2004, 11 (6) :502-504.巩鹏, 王忠裕, 赵作伟, 等.梗阻性黄疸大鼠心肌TNF-α及SOD基因mRNA的表达[J].中国普外基础与临床杂志, 2004, 11 (6) :502-504.
    [4]Yao N, Li YM, Zhou B.Expression and significance of bax and bcl-2 in myocardial tissue injury after obstructive jaundice[J].J LanzhouUniv (Med SCi) , 2007, 33 (3) :31-33. (in Chinese) 姚南, 李玉明, 周斌.Bax、bcl-2在梗阻性黄疸心肌损伤中的表达及意义[J].兰州大学学报 (医学版) , 2007, 33 (3) :31-33.
    [5]Yan HM, Chen WL.Changes of Cytokines in Peritoneal macropha-ges and blood plasma in obstructive jaundice rats[J].J Shanxi MedUniv, 2003, 34 (1) :26-27. (in Chinese) 阎慧明, 陈文亮.梗阻性黄疸大鼠巨噬细胞功能变化的研究[J].山西医科大学学报, 2003, 34 (1) :26-27.
    [6]Dai F, Wang WX, Ding YM, et al.Relationship between TNF-αandhepatic cell apoptosis in obstructive jaundice rats[J].Chin J ExpSurg, 2003, 20 (1) :51-52.戴锋, 王卫星, 丁佑铭, 等.大鼠梗阻性黄疸时肝细胞凋亡与肿瘤坏死因子的关系[J].中华实验外科杂志, 2003, 20 (1) :51-52. (in Chinese)
    [7]Liang XM, Shao TW, Chen CH.Pathological damage and physiologicalprotection of bilirubin in human body[J].Pract J Chin Med, 2012, 9 (3) :172-175. (in Chinese) 梁小明, 邵天伟, 陈昌辉.胆红素对机体的病理损伤和生理保护作用[J].实用医院临床杂志, 2012, 9 (3) :172-175.
    [8]赵国华, 高燕勤, 胡玲.足月新生儿高胆红素血症对心肌损害临床研究[J].河北医药, 2011, 33 (6) :908.
    [9]蒲秀红, 郭晓清, 安涛, 等.新生儿病理性黄疸心肌酶及肌钙蛋白I检测结果分析[J].临床军医杂志, 2010, 38 (5) :779-781.
    [10]张怡, 段司凤, 朱进秋, 等.新生儿高胆红素血症与心肌损伤的临床研究[J].中国优生与遗传杂志, 2010, 18 (8) :82-83.
    [11]张秋丽, 王洪波, 周建国, 等.高胆红素血症对新生儿心肌酶谱的影响[J].宁夏医学杂志, 2010, 32 (6) :567-568.
    [12]顾丹凤.新生儿黄疸心肌损伤的研究[J].海南医学, 2011, 22 (2) :42-43.
    [13]熊金凤, 王义光, 杨兴林, 等.血清胆红素与肌钙蛋白I关系的临床分析[J].检验医学与临床, 2007, 4 (12) :1182-1183.
    [14]朱传伟, 谷圣常, 周庆振.梗阻性黄疸对心肌损伤的临床探讨[J].中国当代医药, 2009, 16 (10) :158-159.
    [15]何小蓉, 涂干卿.心脏标志物的检验医学与临床应用[J].重庆医学, 2009, 38 (10) :1255-1258.
    [16]Xiong XM, Deng SX.Expression of cTnT in rabbit hearts duringmyocardial is chemia and its Post-Mortem stability[J].J ForensicMed, 2008, 24 (2) :15-18, 86. (in Chinese) 熊小明, 邓世雄.心肌肌钙蛋白T在家兔缺血心肌中的表达及其死后稳定性[J].法医学杂志, 2008, 24 (2) :15-18, 86.
    [17]王天然, 周纬, 黄海, 等.可逆型梗阻性黄疸动物模型探讨[J].世界华人消化杂志, 2005, 13 (12) :1454-1456.
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