Association between single nucleotide polymorphism of microRNA-146a and recurrence after liver cancer surgery

Objective To investigate the association between the single nucleotide polymorphism( SNP) of miRNA- 146 a rs2910164 and recurrence after liver cancer surgery. Methods A total of 89 patients with primary liver cancer who underwent radical resection for liver cancer in Maojian Hospital of Dongfeng Medical Group were enrolled,and according to the presence or absence of postoperative recurrence,they were divided into recurrence group and non- recurrence group. The Taq Man probe method was used to determine the genotypes( G / C)of miRNA- 146 a rs2910164. The frequency of each genotype was compared between the two groups to investigate the association between the SNP of MicroRNA- 146 a and recurrence after liver cancer surgery. The independent- samples t test was used for comparison of continuous data between the two groups,the chi- square test was used for comparison of categorical data between the two groups,and multivariate logistic regression analysis was used to determine the factors associated with the recurrence of liver cancer. Results The three genotypes of miRNA- 146 a G / C in non- recurrence group conformed to the Hardy- Weinberg equilibrium law( P > 0. 05). After the surgery for liver cancer,the frequency of miRNA- 146 a G / C genotype showed a significant difference between the recurrence group and the non- recurrence group( χ2= 9. 115,P = 0. 010),and compared with the non- recurrence group,the recurrence group had a significantly higher frequency of miRNA- 146a( rs2910164) CG genotype( χ2= 4. 013,P = 0. 039) and a significantly lower frequency of GG genotype( χ2= 9. 046,P = 0. 003). The multivariate logistic regression analysis showed that tumor diameter( OR = 1. 075,P = 0. 003 9) and miRNA- 146a( rs2910164) CG genotype( OR = 6. 215,P = 0. 001 4) were the risk factors for recurrence after the surgery for liver cancer,and that miRNA146a( rs2910164) GG genotype was the protective factor against recurrence after the surgery for liver cancer( OR = 0. 382,P = 0. 002 5).Conclusion The SNP of MiRNA- 146a( rs2910164 C / G) is associated with recurrence after the radical surgery for liver cancer,and the CG genotype of rs2910164 may be the risk factor for postoperative recurrence after the surgery for liver cancer.