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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 38 Issue 6
Jun.  2022
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Article Navigation >  Journal of Clinical Hepatology  > 2022  >  38(6): 1317-1322

Value of multi-glycan in the auxiliary diagnosis of dual-phenotype hepatocellular carcinoma

DOI: 10.3969/j.issn.1001-5256.2022.06.019
  • 1.

    Department of Clinical Laboratory, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China

  • 2.

    Department of Laboratory Medicine, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

Research funding:

Sponsored by Fuzhou Health Technology Project (2019-S-wq19);

Startup Fund for Scientific Research, Fujian Medical University (2019QH1296);

Fujian Provincial Natural Science Foundation Projects (general) (2020J011169)

More Information
  • Corresponding author: LIU Lijuan, ljliu@126.com (ORCID:0000-0001-9809-0056)
  • Received Date: 2021-09-27
  • Accepted Date: 2021-12-23
  • Published Date: 2022-06-20
    Abstract
  •   Objective  To investigate the expression of multi-glycan in serum of patients with dual-phenotype hepatocellular (DPHCC) and its clinical significance.  Methods  Serum samples were collected from 65 patients with DPHCC, 80 patients with primary hepatocellular carcinoma (HCC), and 120 patients with liver cirrhosis (LC) who were treated in Mengchao Hepatobiliary Hospital of Fujian Medical University from June 2019 to December 2020. DNA sequencer-aided fluorophore-assisted carbohydrate electrophoresis was used to measure the expression of N-glycan in serum, The measurement data of normal distribution were compared by t-test between the two groups and analysis of variance between multiple groups; The measurement data with non normal distribution were compared by Mann-Whitney U test between the two groups and Kruskal-Wallis H test between multiple groups, the chi-square test was used for comparison of categorical data between groups.The logistic regression method was used to establish the common index model. The efficacy of AFP, PIVKA - Ⅱ, CEA, CA19-9 and multi glycan in the diagnosis of DPHCC was evaluated by receiver operating characteristic (ROC) curve, and the area under ROC curve (AUC) was compared by Z test.  Results  There was a significant difference in multi-glycan between the DPHCC group and the HCC group (P < 0.001), while there were no significant differences in AFP, PIVKA-Ⅱ, CEA, CA19-9, and SUM between the two groups (P=0.924, 0.084, 0.442, 0.924, and 0.206). Multi-glycan had an area under the ROC curve (AUC) of 0.775, which was significantly higher than that of AFP (0.507), PIVKA-Ⅱ (0.584), CEA (0.537), CA19-9 (0.505), and SUM (0.561), and multi-glycan had a sensitivity of 69.23%, which was increased compared with the other 5 items. There were significant differences in multi-glycan, AFP, PIVKA-Ⅱ, CA19-9, and SUM between the DPHCC group and the LC group (all P < 0.001), but there was no significant difference in CEA between the two groups (P=0.14). Multi-glycan had an AUC of 0.780, which was also higher than that of AFP (0.767), PIVKA-Ⅱ (0.743), CEA (0.566), CA19-9 (0.689), and SUM (0.713), and multi-glycan had a sensitivity of 89.23%, which was increased compared with the other five items.  Conclusion  Multi-glycan can be used as one of the indicators for the auxiliary diagnosis of DPHCC.

     

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