Clinical effect of fecal microbiota transplantation versus the traditional Chinese medicine <i>Rheum officinale</i> in a rat model of hyperlipidemic acute pancreatitis

Xujuan LUO; Xue BAI; Zenghui LI; Fan LIU; Hao TANG; Ruoxin LI; Guodong YANG

doi:10.3969/j.issn.1001-5256.2022.12.016

Volume 38 Issue 12

Dec. 2022

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Article Navigation > Journal of Clinical Hepatology > 2022 > 38(12): 2767-2773

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Clinical effect of fecal microbiota transplantation versus the traditional Chinese medicine Rheum officinale in a rat model of hyperlipidemic acute pancreatitis

DOI: 10.3969/j.issn.1001-5256.2022.12.016

Xujuan LUO^{1a
,
,
,},
Xue BAI²,
Zenghui LI^1b,
Fan LIU^1a,
Hao TANG^1b,
Ruoxin LI^1b,
Guodong YANG^1a

1a.
Department of Gastroenterology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China
1b.
Medical Imaging Key Laboratory of Sichuan Province, Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China
2.
North Sichuan Medical College, Nanchong, Sichuan 637000, China

Research funding:

Sichuan Provincial Administration of Traditional Chinese Medicine Special Research on Traditional Chinese Medicine (2020JC0056);

The University-Level Scientific Research Development Project of North Sichuan Medical College (CBY20-QA-Y19);

Research and Development Project of the Affiliated Hospital of North Sichuan Medical College (2021JC033);

Research and Development Project of the Affiliated Hospital of North Sichuan Medical College (2022ZD005)

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Corresponding author: LUO Xujuan, luoxujuan1@outlook.com (ORCID: 0000-0003-2192-837X)
Received Date: 2022-09-03
Accepted Date: 2022-10-08
Published Date: 2022-12-20

Abstract

Abstract

Objective To investigate the effect of fecal microbiota transplantation (FMT) on a rat model of hypertriglyceridemic acute pancreatitis (HLAP). Methods A total of 72 male Sprague-Dawley rats were randomly divided into sham-operation group, model group, Rheum officinale group, and fecal microbiota group, with 18 rats in each group. After 8 weeks of feeding with high-fat diet, the rats in the sham-operation group were given sham operation, and those in the other three groups were given retrograde pancreaticobiliary injection of 5% sodium taurocholate to induce acute pancreatitis; after modeling, the rats in the Rheum officinale group were given enema with Rheum officinale, and those in the fecal microbiota group were given enema with fresh fecal microbiota solution. Blood, pancreatic, and terminal ileal tissue samples were collected at 6, 24, and 36 hours after surgery. HE staining was used to observe histopathological changes of the pancreas and the intestine; an automatic biochemical analyzer was used to measure the serum levels of amylase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HLD-C); ELISA was used to measure the serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and endotoxin as an index for intestinal permeability. A one-way analysis of variance was used for comparison of normally distributed continuous data between multiple groups, and the least significant difference t-test or the Tamhane T2 test was used for further comparison between two groups; the Kruskal-Wallis H test was used for comparison of non-normally distributed continuous data between multiple groups, and the Bonferroni method was used for further comparison between two groups. Results Compared with the sham-operation group, the Rheum officinale group and the fecal microbiota group had no significant increase in the pathological score of the terminal ileum at 6 and 24 hours, and there was no significant difference between the fecal microbiota group and the sham-operation group at 36 hours (all P > 0.05). At 36 hours, the Rheum officinale group and the fecal microbiota group had a significantly lower serum level of amylase than the model group (all P < 0.05). Compared with the model group, the Rheum officinale group had a significantly lower serum level of ALT at 36 hours (P < 0.05) and a significantly lower serum level of AST at 24 hours (P < 0.05), while the fecal microbiota group had a significantly lower level of ALT at each time point (P < 0.05) and a significantly lower serum level of AST at 24 and 36 hours (all P < 0.05). The Rheum officinale group and the fecal microbiota group had significant reductions in the serum levels of TC and TG (all P < 0.05); compared with the Rheum officinale group, the fecal microbiota group had a significantly higher serum level of HDL-C at 24 and 36 hours (all P < 0.05), and compared with the model group, the fecal microbiota group had a significantly lower serum level of HDL-C at each time period (all P < 0.05). There were no significant differences in the inflammatory indices IL-6 and TNF-α between the fecal microbiota group and the sham-operation group at each time point (all P > 0.05), and the Rheum officinale group had significantly higher levels than the sham-operation group (all P < 0.05); both the Rheum officinale group and the fecal microbiota group had a significantly lower serum level of endotoxin than the model group (all P < 0.05), and the fecal microbiota group had a significantly lower level of endotoxin than the Rheum officinale group within 6 hours of treatment (P < 0.05). Conclusion Both Rheum officinale and fecal microbiota transplantation can improve tissue inflammation and intestinal permeability in HLAP rats and can improve lipid metabolism and alleviate the progression of pancreatitis to a certain extent, and fecal microbiota transplantation shows a better clinical effect than Rheum officinale alone, but more randomized controlled trials are needed for further investigation.
- Hyperlipidemias,
- Pancreatitis,
- Rheum,
- Fecal Bacteria Transplantation,
- Rats, Sprague-Dawley

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References(19)

References

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Clinical effect of fecal microbiota transplantation versus the traditional Chinese medicine Rheum officinale in a rat model of hyperlipidemic acute pancreatitis

DOI: 10.3969/j.issn.1001-5256.2022.12.016