Protective effect of prostaglandin E1 pretreatment against liver ischemia-reperfusion injury in rats with cholestasis

Xu Feng; Liu XiaoLin; Wang Chao; Dai ChaoLiu

doi:10.3969/j.issn.1001-5256.2017.05.022

Issue 5

May 2017

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > Journal of Clinical Hepatology > 2017 > 33(5): 899-904

PDF( 2639 KB)

Protective effect of prostaglandin E1 pretreatment against liver ischemia-reperfusion injury in rats with cholestasis

DOI: 10.3969/j.issn.1001-5256.2017.05.022

Department of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University

Research funding:

Published Date: 2017-05-20

Abstract

Abstract

Objective To investigate the protective mechanism of prostaglandin E1 (PGE1) against liver ischemia-reperfusion injury in rats with cholestasis.Methods A total of 36 male Wistar rats were randomly divided into PGE1 group (PGE group) and normal saline group (NS group) .The rats in the PGE group were treated with continuous pump of PGE1 (0.5 μg/kg/min) from 15 minutes before liver ischemia to 60 minutes of reperfusion, and those in the NS group were given normal saline of the same volume.Common bile duct ligation was performed to establish a rat model of cholestasis.Seven days later, Pringle maneuver was used to perform hepatic inflow occlusion for 15 minutes, and serum levels of enzymes and bilirubin were measured at 1, 6, and 24 hours of reperfusion, as well as the levels of myeloperoxidase (MPO) , tumor necrosis factor α (TNFα) , Bcl-2, Bax, and human heat shock protein 70 (HSP70) and histopathological changes.Results At 1, 6, and 24 hours of reperfusion, there were no significant differences in total bilirubin and direct bilirubin between the two groups (both P>0.05) , and the PGE group had significantly lower levels of alanine aminotransferase, aspartate aminotransferase, MPO, and TNFα than the NS group (all P<0.05) .At 1, 6, and 24 hours of reperfusion, compared with the NS group, the PGE group had a significantly higher level of Bcl-2 and a significantly lower level of Bax (both P<0.05) .At 1 and 6 hours of reperfusion, the PGE group had significantly higher mRNA expression of HSP70 than the NS group (P<0.05) 24="" and="" at="" hours="" of="" there="" was="" no="" significant="" difference="" in="" mrna="" expression="" hsp70="" between="" the="" two="" groups="" p="">0.05) .Compared with the NS group, the PGE group had a lower degree of liver injury, which manifested as reduced hepatocyte swelling and necrosis, clear structures of the hepatic cords and the hepatic sinusoids, regular arrangement of hepatic cords, and widened hepatic sinusoids.Conclusion PGE1 protects the liver with cholestasis against ischemia-reperfusion injury by reducing neutrophil infiltration and Bax expression and upregulating the expression of HSP70 and Bcl-2.
- cholestasis,
- reperfusion injury,
- alprostadil,
- rats, wistar

FullText(HTML)

References(22)

References

[1]KLOEK JJ, MARECHAL X, ROELOFSEN J, et al.Cholestasis is associated with hepatic microvascular dysfunction and aberrant energy metabolism before and during ischemia-reperfusion[J].Antioxid Redox Signal, 2012, 17 (8) :1109-1123.

[2]KLOEK JJ, MARSMAN HA, van VLIET AK, et al.Biliary drainage attenuates postischemic reperfusion injury in the cholestatic rat liver[J].Surgery, 2008, 144 (1) :22-31.

[3]XU FG, GU JY, BAI JL, et al.Meta-analysis on the efficacy and safety of preoperative biliary drainage in patients with obstructive jaundice[J].Chin J General Surg, 2011, 20 (2) :123-128. (in chinese) 徐方贵, 顾劲扬, 柏建岭, 等.恶性阻塞性黄疸术前胆道引流的有效性和安全性的荟萃分析[J].中国普通外科杂志, 2011, 20 (2) :123-128.

[4]MAIDA K, AKAMATSU Y, HARA Y, et al.Short oxygenated warm perfusion with prostaglandin E1 administration before cold preservation as a novel resuscitation method for liver grafts from donors after cardiac death in a rat in vivo model[J].Transplantation, 2016, 100 (5) :1052-1058.

[5]XU F, DAI CL, PENG SL, et al.Preconditioning with glutamine protects against ischemia/reperfusion-induced hepatic injury in rats with obstructive jaundice[J].Pharmacology, 2014, 93 (3-4) :155-165.

[6]LIU YI, LIU Z, CHEN Y, et al.PPARgamma activation reduces ischemia/reperfusion-induced metastasis in a murine model of hepatocellular carcinoma[J].Exp Ther Med, 2016, 11 (2) :387-396.

[7]YOSHIMOTO K, TAJIMA H, OHTA T, et al.Increased E-selectin in hepatic ischemia-reperfusion injury mediates liver metastasis of pancreatic cancer[J].Oncol Rep, 2012, 28 (3) :791-796.

[8]KNUDSEN AR, ANDERSEN KJ, HAMILTON-DUTOIT S, et al.Correlation between liver cell necrosis and circulating alanine aminotransferase after ischaemia/reperfusion injuries in the rat liver[J].Int J Exp Pathol, 2016, 97 (2) :133-138.

[9]TEOH N, FIELD J, SUTTON J, et al.Dual role of tumor necrosis factor-alpha in hepatic ischemia-reperfusion injury:studies in tumor necrosis factor-alpha gene knockout mice[J].Hepatology, 2004, 39 (2) :412-421.

[10]WANG YZ, MING YZ, XIONG L, et al.Protective effects of prostaglandin E1 in rat hepatic ischemia/reperfusion injury[J].Chin JGeneral Surg, 2008, 17 (1) :41-44. (in chinese) 王玉柱, 明英姿, 熊力, 等.前列腺素E1对大鼠肝脏缺血再灌注损伤的保护作用[J].中国普通外科杂志, 2008, 17 (1) :41-44.

[11]SCHROR K, HOHLFELD T.Mechanisms of anti-ischemic action of prostaglandin E1 in peripheral arterial occlusive disease[J].Vasa, 2004, 33 (3) :119-124.

[12]HAFEZ T, MOUSSA M, NESIM I, et al.The effect of intraportal prostaglandin E1 on adhesion molecule expression, inflammatory modulator function, and histology in canine hepatic ischemia/reperfusion injury[J].J Surg Res, 2007, 138 (1) :88-99.

[13]MAHMOUD MF, EL SHAZLY SM, BARAKAT W.Inhibition of TNF-alpha protects against hepatic ischemia-reperfusion injury in rats via NF-kappa B dependent pathway[J].N-S Arch Pharmacol, 2012, 385 (5) :465-471.

[14]LI J, KE W, ZHOU Q, et al.Tumour necrosis factor-alpha promotes liver ischaemia-reperfusion injury through the PGC-1alpha/Mfn2 pathway[J].J Cell Mol Med, 2014, 18 (9) :1863-1873.

[15]ZAPLETAL C, FALLSEHR C, REIDEL M, et al.Induction o HSP70 shows differences in protection against I/R injury derived by ischemic preconditioning and intermittent clamping[J].Microvasc Res, 2010, 80 (3) :365-371.

[16]BOERI D, DONDERO F, STORACE D, et al.Heat-shock protein 70 favours human liver recovery from ischaemia-reperfusion[J].Eur J Clin Invest, 2003, 33 (6) :500-504.

[17]LEE LY, KAIZU T, TOYOKAWA H, et al.Carbon monoxide induces hypothermia tolerance in Kupffer cells and attenuates liver ischemia/reperfusion injury in rats[J].Liver Transpl, 2011, 17 (12) :1457-1466.

[18]CALVISI DF, LADU S, HIRONAKA K, et al.Vitamin E downmodulates i NOS and NADPH oxidase in c-Myc/TGF-alpha transgenic mouse model of liver cancer[J].J Hepatol, 2004, 41 (5) :815-822.

[19]KUME M, YAMAMOTO Y, SAAD S, et al.Ischemic preconditioning of the liver in rats:implications of heat shock protein induction to increase tolerance of ischemia-reperfusion injury[J].J Lab Clin Med, 1996, 128 (3) :251-258.

[20]STANKIEWICZ AR, LACHAPELLE G, FOO CP, et al.Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation[J].J Biol Chem, 2005, 280 (46) :38729-38739.

[21]BEN-ARI Z, PAPPO O, CHEPORKO Y, et al.Bax ablation protects against hepatic ischemia/reperfusion injury in transgenic mice[J].Liver Transpl, 2007, 13 (8) :1181-1188.

[22]HARDWICK JM, SOANE L.Multiple functions of BCL-2 family proteins[J].Cold Spring Harb Perspect Biol, 2013, 5 (2) .pii:a008722.

Relative Articles

[1]	Du YiChao, Zhang Hao, Zhong FuRui, Cheng HuanLi, Lai Li, Qian BaoLin, Tan Peng, Xia XianMing, Fu WenGuang. Protective effect of pinocembrin in a mouse model of liver injury induced by acetaminophen[J]. Journal of Clinical Hepatology, 2020, 36(3): 608-611. doi: 10.3969/j.issn.1001-5256.2020.03.027
[3]	Li Yang, Shu DeJun, Peng CiJun, Feng ZanJie, Mei Yong, Xiong Kun. Effect of erythropoietin on the mRNA expression of apoptosis genes bcl-2 and bax in rats with fatty liver disease after hepatic ischemia-reperfusion injury[J]. Journal of Clinical Hepatology, 2019, 35(1): 157-160. doi: 10.3969/j.issn.1001-5256.2019.01.030
[4]	Lu Xin, Liu Ying, Jia Lei, Kong LingYu, Liu DianQing. Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice[J]. Journal of Clinical Hepatology, 2019, 35(7): 1532-1535. doi: 10.3969/j.issn.1001-5256.2019.07.022
[5]	Wang Hao, He Cheng, Chen Hao, Ou YangZhongMin, Liu ZhiQiang. Value of Gd-EOB-DTPA-enhanced MRI in the assessment of liver function in dogs with acute liver failure[J]. Journal of Clinical Hepatology, 2019, 35(7): 1576-1581. doi: 10.3969/j.issn.1001-5256.2019.07.030
[6]	Zhang YongChao, Bi YanZhen, Fang Xiao, Wang QuanYi, Wang QuanQuan, Tang HuiXin, Kong LingBin, Chen Yu, Duan ZhongPing, Shu ZhenFeng, Hong Feng. Protective effect of probiotics in rats with acute-on-chronic liver failure and related mechanism[J]. Journal of Clinical Hepatology, 2019, 35(7): 1570-1575. doi: 10.3969/j.issn.1001-5256.2019.07.029
[7]	Wu LinLan, Xu Bing, Wei JianWei, Zhao ZhiPing, Huang SuQin, Yang XiaoMei. Therapeutical effect of local transplantation of bone marrow mesenchymal stem cells mixed with fibrin gel on rats with liver injury[J]. Journal of Clinical Hepatology, 2018, 34(8): 1740-1744. doi: 10.3969/j.issn.1001-5256.2018.08.029
[8]	Pan Qi, Luo ChunHai, Ma WanLing, Qu YuanFei, Wang JianRu, Chen Lin. Specific magnetic resonance imaging of vascular endothelial growth factor-C targeted molecular probe and its clinical significance in a rat model of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2018, 34(7): 1492-1496. doi: 10.3969/j.issn.1001-5256.2018.07.025
[9]	Liang Kai, Kong WeiZong, Chen Juan, Wang YingChun. Effect of trefoil factor 3 on intestinal mucous barrier in rats with nonalcoholic steatohepatitis[J]. Journal of Clinical Hepatology, 2017, 33(8): 1552-1557. doi: 10.3969/j.issn.1001-5256.2017.08.029
[10]	Shi HongBo, Shi HongLin, Zhang XiangYing, Chen DeXi, Duan ZhongPing, Ren Feng. Protective effect of autophagy in mice with acute liver injury induced by D- galactosamine / lipopolysaccharide and related mechanisms[J]. Journal of Clinical Hepatology, 2017, 33(2): 329-333. doi: 10.3969/j.issn.1001-5256.2017.02.027
[11]	Li XiaoTian, Yin Yan, Guo YongZe, Zhou LiYun, You ZiXuan. Mechanism of action of 1,25(OH)_2D_3 in influencing the expression of interleukin-17 and macrophage inflammatory protein-3α and inhibiting the formation of liver fibrosis in rats[J]. Journal of Clinical Hepatology, 2016, 32(12): 2331-2336. doi: 10.3969/j.issn.1001-5256.2016.12.020
[12]	Li Dong, Lu ZhongHua, Gan JianHe. Establishment of a rat model of early-stage liver failure and Th17/Treg imbalance[J]. Journal of Clinical Hepatology, 2016, 32(5): 928-932. doi: 10.3969/j.issn.1001-5256.2016.05.025
[13]	Li XiaoPeng, Li Ming, Lei Wan, Zhang LunLi. Protective effect of magnesium isoglycyrrhizinate in rats with acute liver failure induced by D- galactosamine[J]. Journal of Clinical Hepatology, 2016, 32(3): 545-548. doi: 10.3969/j.issn.1001-5256.2016.03.032
[14]	Lai JieYing, Song SiYu, Hao LiPing, Zhang XiaoLi. Localization and therapeutic effect of bone marrow hematopoietic cell transplantation in rat model of liver fibrosis[J]. Journal of Clinical Hepatology, 2015, 31(11): 1878-1882. doi: 10.3969/j.issn.1001-5256.2015.11.026
[15]	Xu ZhongNan, Wu XiMing, Wang Pei, Zhang XiQuan, Xia ChunGuang, Wang XunQiang, Wang Yang, Zhan XiaoLe, Qu Ying, Xu MingYi, Lu LunGen. 18α glycyrrhizic acid in the treatment of acute liver injury induced by D-galactosamine in rats[J]. Journal of Clinical Hepatology, 2012, 28(1): 63-65+70.
[16]	Lu XiaoJu, Chen YongPing, Yang Tao, Shen ChunYan. Effect of baicalin of the expression of NF-κB in acute liver failure in rats[J]. Journal of Clinical Hepatology, 2011, 27(10): 1087-1092.
[17]	Wang JiPing, Leng JiYan, Dong Dong, Yan WeiQun. The protective effect of rhKD/APP on liver injured by D-galactosamine in rats[J]. Journal of Clinical Hepatology, 2007, 23(1): 24-25.
[19]	Wang JunXue, Wang GuoJun, Cai Xiong. The effects of oxymatrine on apoptosis induced by TNF-α in human liver cell line L02[J]. Journal of Clinical Hepatology, 2001, 17(4): 210-211+217.

Supplements(0)

Cited By

Cited by

Periodical cited type(6)

1.	黄成，崔海坡，谭蔚锋，芦映希，姜赫宣. 肝门阻断夹持器齿型对夹持性能的影响. 生物医学工程学进展. 2022(03): 139-143 .
2.	吴均政. 腹腔镜Pringle法联合左肝静脉阻断对左半肝切除术患者术中出血量及凝血指标的影响. 中外医学研究. 2021(26): 45-48 .
3.	石川，常华，豆松萌，李恒. Pringle联合IVC法在巨大肝血管瘤切除术中的应用效果分析. 河南外科学杂志. 2021(06): 30-33 .
4.	汪长青，吴珍宝. 不同肝血流阻断对腹腔镜肝切除术患者的影响. 西南国防医药. 2019(02): 116-118 .
5.	刘雄友，高德山，郭忠涛. 不同肝血流阻断方式对肝切除术安全性及预后的影响. 肝胆外科杂志. 2018(01): 42-46 .
6.	殷杰，徐新宝，朱日祥. 腹腔镜肝切除术治疗肝血管瘤58例临床分析. 中华肝脏外科手术学电子杂志. 2018(06): 469-472 .

Other cited types(2)

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views (3045) PDF downloads(464)

Protective effect of prostaglandin E1 pretreatment against liver ischemia-reperfusion injury in rats with cholestasis

DOI: 10.3969/j.issn.1001-5256.2017.05.022