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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 39 Issue 8
Aug.  2023
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Article Navigation >  Journal of Clinical Hepatology  > 2023  >  39(8): 1848-1856

Differentially expressed microRNAs in plasma exosomes from patients with chronic hepatitis B or hepatitis B virus-related acute-on-chronic liver failure: A bioinformatics analysis

DOI: 10.3969/j.issn.1001-5256.2023.08.013
  • 1.

    Qingdao Clinical Medical College of Nanjing Medical University, Qingdao, Shandong 266011, China

  • 2.

    Department of Infectious Diseases, Qingdao Municipal Hospital, Qingdao, Shandong 266011, China

  • 3.

    Clinical Research Center, Qingdao Municipal Hospital, Qingdao, Shandong 266071, China

  • 4.

    Department of Blood Purification, Qingdao Sixth People's Hospital, Qingdao, Shandong 266033, China

Research funding:

National Natural Science Foundation of China (82202416);

Special Fund for Artificial Liver of Beijing Public Welfare Foundation (RGGJJ-2021-030)

More Information
  • Corresponding author: LIU Sheng, lius123@163.com (ORCID: 0000-0002-9485-0211); XIN Yongning, xinyongning@163.com (ORCID: 0000-0002-3692-7655)
  • Received Date: 2022-12-07
  • Accepted Date: 2023-01-17
  • Published Date: 2023-08-20
    Abstract
  •   Objective  To investigate the differences in plasma exosomal microRNA (miRNA) expression profile between patients with chronic hepatitis B (CHB) and those with hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF), as well as their functions and biological processes, and to provide a reference for the clinical diagnosis of HBV-ACLF.  Methods  Six patients with CHB who were hospitalized in Department of Infectious Diseases, Qingdao Municipal Hospital, and six patients with HBV-ACLF who were treated in Blood Purification Center of Qingdao Sixth People's Hospital from October 2021 to June 2022 were enrolled. Illumina high-throughput sequencing was used to analyze the plasma exosomal miRNAs of these patients to obtain the differentially expressed miRNAs between the two groups. The differentially expressed miRNAs were screened, and a functional enrichment analysis was performed to identify the biological processes involving such miRNAs. The Mann-Whitney U rank sum test was used for comparison of continuous data between two groups, and the chi-square test was used for comparison of categorical data between groups.  Results  A total of 249 differentially expressed exosomal miRNAs were obtained according to the criteria of upregulation >2-fold or downregulation >2-fold, with P < 0.05, and compared with the CHB group, there were 126 upregulated miRNAs and 123 downregulated miRNAs. The bioinformatics analysis showed that these differentially expressed miRNAs were mainly involved in the biological processes such as gonadal development, regulation of protein stability, and cellular response to external stimuli, and they were closely associated with the signaling pathways such as hepatitis B, the role of proteoglycans in cancer, regulation of stem cell pluripotency, MAPK, Hippo, TNF, and lipid metabolism.  Conclusion  The differentially expressed miRNAs identified by Illumina high-throughput sequencing may be used as biomarkers for the early diagnosis and prognostic evaluation of HBV-ACLF.

     

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    • References(26)
  • [1]
    TANG Y, LIANG H, ZENG G, et al. Advances in new antivirals for chronic hepatitis B[J]. Chin Med J (Engl), 2022, 135(5): 571-583. DOI: 10.1097/CM9.0000000000001994.
    [2]
    ARROYO V, MOREAU R, JALAN R. Acute-on-chronic liver failure[J]. N Engl J Med, 2020, 382(22): 2137-2145. DOI: 10.1056/NEJMra1914900.
    [3]
    CONSOLE L, SCALISE M, INDIVERI C. Exosomes in inflammation and role as biomarkers[J]. Clin Chim Acta, 2019, 488: 165-171. DOI: 10.1016/j.cca.2018.11.009.
    [4]
    Chinese Society of Infectious Diseases, Chinese Medical Association; Chinese Society of Hepatology, Chinese Medical Association. Guidelines for the prevention and treatment of chronic hepatitis B(version 2019)[J]. J Clin Hepatol, 2019, 35(12): 2648-2669. DOI: 10.3969/j.issn.1001-5256.2019.12.007.

    中华医学会感染病学分会, 中华医学会肝病学分会. 慢性乙型肝炎防治指南(2019年版)[J]. 临床肝胆病杂志, 2019, 35(12): 2648-2669. DOI: 10.3969/j.issn.1001-5256.2019.12.007.
    [5]
    Liver Failure and Artificial Liver Group, Chinese Society of Infectious Diseases, Chinese Medical Association; Severe Liver Disease and Artificial Liver Group, Chinese Society of Hepatology, Chinese Medical Association. Guideline for diagnosis and treatment of liver failure(2018)[J]. J Clin Hepatol, 2019, 35(1): 38-44. DOI: 10.3969/j.issn.1001-5256.2019.01.007.

    中华医学会感染病学分会肝衰竭与人工肝学组, 中华医学会肝病学分会重型肝病与人工肝学组. 肝衰竭诊治指南(2018年版)[J]. 临床肝胆病杂志, 2019, 35(1): 38-44. DOI: 10.3969/j.issn.1001-5256.2019.01.007.
    [6]
    GURUNATHAN S, KANG MH, JEYARAJ M, et al. Review of the isolation, characterization, biological function, and multifarious therapeutic approaches of exosomes[J]. Cells, 2019, 8(4): 307. DOI: 10.3390/cells8040307.
    [7]
    MORI MA, LUDWIG RG, GARCIA-MARTIN R, et al. Extracellular miRNAs: from biomarkers to mediators of physiology and disease[J]. Cell Metab, 2019, 30(4): 656-673. DOI: 10.1016/j.cmet.2019.07.011.
    [8]
    ZHANG J, LI S, LI L, et al. Exosome and exosomal microRNA: trafficking, sorting, and function[J]. Genomics Proteomics Bioinformatics, 2015, 13(1): 17-24. DOI: 10.1016/j.gpb.2015.02.001.
    [9]
    CHEN W, YAN ZH, WANG YM, et al. Genome-wide microarray-based analysis of miRNAs expression in patients with acute-on-chronic liver failure[J]. Hepatobiliary Pancreat Dis Int, 2014, 13(1): 32-39. DOI: 10.1016/s1499-3872(14)60004-7.
    [10]
    HU J, XU Y, HAO J, et al. MiR-122 in hepatic function and liver diseases[J]. Protein Cell, 2012, 3(5): 364-371. DOI: 10.1007/s13238-012-2036-3.
    [11]
    TANIMIZU N, KOBAYASHI S, ICHINOHE N, et al. Downregulation of miR122 by grainyhead-like 2 restricts the hepatocytic differentiation potential of adult liver progenitor cells[J]. Development, 2014, 141(23): 4448-4456. DOI: 10.1242/dev.113654.
    [12]
    TU WL, YOU LR, TSOU AP, et al. Pten haplodeficiency accelerates liver tumor growth in miR-122a-Null mice via expansion of periportal hepatocyte-like cells[J]. Am J Pathol, 2018, 188(11): 2688-2702. DOI: 10.1016/j.ajpath.2018.07.019.
    [13]
    KRAUSKOPF J, CAIMENT F, CLAESSEN SM, et al. Application of high-throughput sequencing to circulating microRNAs reveals novel biomarkers for drug-induced liver injury[J]. Toxicol Sci, 2015, 143(2): 268-276. DOI: 10.1093/toxsci/kfu232.
    [14]
    BAI S, NASSER MW, WANG B, et al. MicroRNA-122 inhibits tumorigenic properties of hepatocellular carcinoma cells and sensitizes these cells to sorafenib[J]. J Biol Chem, 2009, 284(46): 32015-32027. DOI: 10.1074/jbc.M109.016774.
    [15]
    JOPLING CL, YI M, LANCASTER AM, et al. Modulation of hepatitis C virus RNA abundance by a liver-specific microRNA[J]. Science, 2005, 309(5740): 1577-1581. DOI: 10.1126/science.1113329.
    [16]
    CHEN S, YANG L, PAN A, et al. Inhibitory effect on the hepatitis B cells through the regulation of miR-122-MAP3K2 signal pathway[J]. An Acad Bras Cienc, 2019, 91(2): e20180941. DOI: 10.1590/0001-3765201920180941.
    [17]
    WEN Y, PENG SF, FU L, et al. Serum levels of miRNA in patients with hepatitis B virus-associated acute-on-chronic liver failure[J]. Hepatobiliary Pancreat Dis Int, 2018, 17(2): 126-132. DOI: 10.1016/j.hbpd.2018.03.004.
    [18]
    WU L, NGUYEN LH, ZHOU K, et al. Precise let-7 expression levels balance organ regeneration against tumor suppression[J]. Elife, 2015, 4: e09431. DOI: 10.7554/eLife.09431.
    [19]
    MCDANIEL K, HUANG L, SATO K, et al. The let-7/Lin28 axis regulates activation of hepatic stellate cells in alcoholic liver injury[J]. J Biol Chem, 2017, 292(27): 11336-11347. DOI: 10.1074/jbc.M116.773291.
    [20]
    MATSUURA K, AIZAWA N, ENOMOTO H, et al. Circulating let-7 levels in serum correlate with the severity of hepatic fibrosis in chronic hepatitis C[J]. Open Forum Infect Dis, 2018, 5(11): ofy268. DOI: 10.1093/ofid/ofy268.
    [21]
    SANDHU GK, MCMILLIN M, FRAMPTON G, et al. Let-7f-dependent suppression of neuronal IGF1 by aberrant TGFβ1 signaling contributes to the neurological decline observed during acute liver failure[J]. J Gastroenterology, 2017, 152(5): S1066. DOI: 10.1016/s0016-5085(17)33601-6.
    [22]
    YANG S, CHEN Z, FAN D, et al. Retracted Article: MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3[J]. RSC Adv, 2018, 8(61): 34973-34983. DOI: 10.1039/c8ra07055e.
    [23]
    CHANDEL R, DAS A, CHAWLA YK, et al. Mo1477 Progression of hepatocellular carcinoma is associated with the up regulation of rno-miR-96/182/183 cluster in liver of wistar rats[J]. J Gastroenterology, 2016, 150(4): S1125. DOI: 10.1016/s0016-5085(16)33799-4
    [24]
    MANDAL R, HARDIN H, BAUS R, et al. Analysis of miR-96 and miR-133a expression in gastrointestinal neuroendocrine neoplasms[J]. Endocr Pathol, 2017, 28(4): 345-350. DOI: 10.1007/s12022-017-9504-5.
    [25]
    SHI Y, JIA M, XU L, et al. miR-96 and autophagy are involved in the beneficial effect of grape seed proanthocyanidins against high-fat-diet-induced dyslipidemia in mice[J]. Phytother Res, 2019, 33(4): 1222-1232. DOI: 10.1002/ptr.6318.
    [26]
    BAJAJ JS, REDDY KR, O'LEARY JG, et al. Serum levels of metabolites produced by intestinal microbes and lipid moieties independently associated with acute-on-chronic liver failure and death in patients with cirrhosis[J]. Gastroenterology, 2020, 159(5): 1715-1730. e12. DOI: 10.1053/j.gastro.2020.07.019.
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