中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 4
Apr.  2016
Turn off MathJax
Article Contents

Protective effect of Cordyceps militaris polypeptide against acute alcoholic liver injury in rats

DOI: 10.3969/j.issn.1001-5256.2016.04.011
Research funding:

 

  • Published Date: 2016-04-20
  • Objective To investigate the protective effect of Cordyceps militaris polypeptide against acute alcoholic liver injury in rats and related mechanism. Methods A total of 60 Wistar rats were randomly divided into blank control group,model group,and low-,medium-,and high- dose Cordyceps militaris polypeptide groups. All rats except those in the blank control group were given 10 ml / kg 56° liquor by gavage once a day; the rats in the blank control group were given distilled water of the same dose by gavage once a day. At 1 hour after gavage with liquor,the rats in the model group and low-,medium-,and high- dose Cordyceps militaris polypeptide groups were given distilled water or Cordyceps militaris polypeptide solution( 6 ml / kg) by gavage. Blood samples were collected from the orbit 4 weeks later.The serum levels of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) and the activity of superoxide dismutase( SOD)and level of malondialdehyde( MDA) in the liver were measured for each group,and the pathological changes in the liver were observed under a light microscope. Analysis of variance was applied for comparison between multiple groups,and the SNK- q test was applied for comparison between any two groups. Results Compared with the model group,the low-,medium-,and high- dose Cordyceps militaris polypeptide groups showed significant reductions in the serum levels of ALT and AST and the level of MDA in the liver( all P < 0. 05),as well as a significant increase in the activity of SOD in the liver( all P < 0. 05),while these indices showed significant differences between the low-,medium-,and high- dose Cordyceps militaris polypeptide groups( all P < 0. 05). The liver pathological sections from the low-,medium-,and high- dose Cordyceps militaris polypeptide groups showed alleviated hepatocyte fatty degeneration and necrosis induced by alcohol under a light microscope. Conclusion Cordyceps militaris polypeptide has a protective effect against acute liver injury induced by alcohol,and its mechanism of action may be related to its antioxidant effect.

     

  • loading
  • [1]ZHENG ZL,HUANG CH,MEI CY,et al.Current advance on the research of cordyceps militaris[J].J Environ Entomol,2011,33(2):225-233.(in Chinese)郑壮丽,黄春花,梅彩英,等.蛹虫草国内外研究的新进展[J].环境昆虫学报,2011,33(2):225-233.
    [2]LIU GJ,ZHOU SJ,YANG SL,et al.Research process of cordycepin in cordyceps militaris[J].Food Sci,2013,34(21):408-413.(in Chinese)刘桂君,周思静,杨素玲,等.蛹虫草中虫草素的研究进展[J].食品科学,2013,34(21):408-413.
    [3]NIE LH,SUN SY,WU ZY,et al.Systematic evaluation on jinshuibao capsule for chronic renal failure[J].Chin J Exp Med Formul,2012,18(11):5-9.(in Chinese)聂玲辉,孙升云,吴志勇,等.金水宝胶囊治疗慢性肾功能衰竭的系统评价[J].中国实验方剂学杂志,2012,18(11):5-9.
    [4]HUANG YL.Effects of Jinshuibao capsule on immunoglobulin and pulmonary function in patients with chronic obstructive pulmonary disease[J].J Emerg Tradit Chin Med,2009,18(10):1589,1605.(in Chinese)黄燕玲.金水宝胶囊对慢性阻塞性肺疾病免疫球蛋白及肺功能的影响[J].中国中医急症,2009,18(10):1589,1605.
    [5]OUYANG YY,ZHANG Z,CAO YR,et al.Effects of cordyceps acid and cordycepin on the inflammatory and fibrogenic response hepatic stellate cells[J].Chin J Hepatol,2013,21(4):275-278.(in Chinese)欧阳阳阳,张哲,曹忆嵘,等.虫草酸和虫草素对肝星状细胞炎症及纤维化发生特性的影响[J].中华肝脏病杂志,2013,21(4):275-278.
    [6]ZHANG N.Cordyceps militaris polypeptide enhance the chemosensitivity of gastric cancer ceil line SGC-7901 on 5-fluorouracil through PI3K/Akt/mT OR pathway[D].Changchun:Jilin Univ,2014.(in Chinese)张楠.蛹虫草多肽通过PI3K/Akt/mT OR通路增强胃癌细胞株SGC-7901对氟尿嘧啶化疗敏感性的研究[D].长春:吉林大学,2014.
    [7]ZHAO M,CHI LP,WANG FY,et al.Establishment of mice acute liver injury model induced by alcohol and its application[J].South China J Prev Med,2005,31(1):14-17.(in Chinese)赵敏,池莉平,王凤岩,等.小鼠急性酒精性肝损伤模型的建立及应用[J].华南预防医学,2005,31(1):14-17.
    [8]SUSSMAN S,DENT CW,SKARA S,et al.Alcoholic liver disease(ALD):a new domain for prevention effort[J].Subst Use Missue,2002,37(14):1887-1904.
    [9]LI YM.The epidemiology and natural history of alcoholic liver diseases[J].Chin J Hepatol,2010,18(3):171-172.(in Chinese)厉有名.酒精性肝病的流行病学及自然史[J].中华肝脏病杂志,2010,18(3):171-172.
    [10]SALMAN N,MUHAMMAD SK,JAVID F,et al.Cirrhosis and its complications:evidence based treatment[J].World J Gastroenterol,2014,20(18):5442-5460.
    [11]EID N,ITO Y,MAEMURA K,et al.Elevated autophagic sequestration of mitochondria and lipid droplets in steatotic hepatocytes of chronic ethanol-treated rats:an immunohistochemical and electron microscopic study[J].J Mol Histol,2013,44(3):311-326.
    [12]LI AH,ZHAO G.Rrearch advances in the pathogenesis of alcoholic liver disease[J].Jilin Med J,2010,31(17):2670-2672.(in Chinese)李爱华,赵刚.酒精性肝病发病机制的研究进展[J].吉林医学,2010,31(17):2670-2672.
    [13]ZHENG JL,QI H,HUANG ZQ,et al.Determination of antioxidant activity of mycelium fermentation broth and sporocarp from northern caterpillar fungus(cordyceps militaris)[J].J Microbiol,2012,32(6):93-95.(in Chinese)郑剑玲,齐贺,黄竹青,等.北虫草菌丝发酵液和子实体抗氧化活性测定[J].微生物学杂志,2012,32(6):93-95.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2427) PDF downloads(430) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return