Role and mechanism of action of the Mongolian medicine <i>Scabiosa atropurea</i> in inhibiting the proliferation of hepatic stellate cells

Yuxin YAN; Xiaoyang GAO; Chunyan ZHANG; Rong JIN; Hongwei YUAN; Yuehong MA

doi:10.3969/j.issn.1001-5256.2023.06.015

Volume 39 Issue 6

Jun. 2023

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Article Navigation > Journal of Clinical Hepatology > 2023 > 39(6): 1351-1357

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Role and mechanism of action of the Mongolian medicine Scabiosa atropurea in inhibiting the proliferation of hepatic stellate cells

DOI: 10.3969/j.issn.1001-5256.2023.06.015

School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010000, China

Research funding:

National Natural Science Foundation of China (81960759);

National Natural Science Foundation of China (81560706);

Natural Science Foundation of Inner Mongolia Autonomous Region (2019MS08010);

Natural Science Foundation of Inner Mongolia Autonomous Region (2014MS0841);

Grassland Talent Cultivation Program of Inner Mongolia Autonomous Region (Human Resources and Social Security Office of Inner Mongolia[2016]348);

Zhiyuan Talent Project of Inner Mongolia Medical University (2020-11);

Inner Mongolia Talent Development Fund (2022-22056);

Science and Technology Innovation Team for Research on the Anti-Hepatic Fibrosis Effects of Mongolian Medicine at Inner Mongolia Medical University (2022-01);

Key Project of Inner Mongolia Medical University (YKD2022ZD019)

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Corresponding author: MA Yuehong, myh19982002@sina.com (ORCID: 0000-0003-0699-378X)
Received Date: 2022-09-10
Accepted Date: 2023-02-01
Published Date: 2023-06-20

Abstract

Abstract

Objective To investigate the role and mechanism of action of Scabiosa atropurea in inhibiting the proliferation of hepatic stellate cells using cell experiment. Methods A total of 20 Wistar rats were randomly divided into control group and administration group, with 10 rats in each group. The rats in the control group were given normal saline by gavage, and those in the administration group were given Scabiosa atropurea by gavage to prepare drug-containing serum. HSC-T6 cells were incubated with the serum from the control group (10%) or the low-, middle-, and high-dose serum containing Scabiosa atropurea (10%, 15%, and 20%, respectively). MTT assay was used to observe the effect of different drug concentrations on cells in different periods of time; flow cytometry was used to measure cell apoptosis; qRT-PCR and Western blot were used to measure the mRNA and protein expression levels of fibrosis markers (α-SMA, collagen Ⅰ) and PI3K/Akt signaling pathway-related factors in hepatic stellate cells (HSCs). A one-way analysis of variance was used for comparison between multiple groups, and the least significant difference t- test was used for further comparison between two groups. Results Compared with the control group, the low-, middle-, and high-dose serum containing Scabiosa atropurea groups had a significant reduction in the OD value of cells (all P < 0.05) and a significant increase in the overall apoptosis rate of cells (all P < 0.05). The results of qRT-PCR showed that compared with the control group, the low-, middle-, and high-dose serum containing Scabiosa atropurea groups had significant reductions in the mRNA expression levels of α-SMA, collagen Ⅰ, PI3K, and Akt and a significant increase in the mRNA expression level of PTEN (all P < 0.05); Western blot showed that compared with the control group, the low-, middle-, and high-dose serum containing Scabiosa atropurea groups had significant reductions in the protein expression levels of α-SMA, collagen Ⅰ, PI3K, Akt, and p-Akt and a significant increase in the protein expression level of PTEN (all P < 0.05). Conclusion The Mongolian medicine Scabiosa atropurea can inhibit the proliferation of HSC-T6 cells and promote their apoptosis, possibly by regulating fibrosis markers and the PI3K/Akt signaling pathway to exert an anti-liver fibrosis effect.
- Hepatic Fibrosis,
- Hepatic Stellate Cells,
- Scabiosa Atropurea,
- Signal Transduction

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References(27)

References

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Role and mechanism of action of the Mongolian medicine Scabiosa atropurea in inhibiting the proliferation of hepatic stellate cells

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Role and mechanism of action of the Mongolian medicine Scabiosa atropurea in inhibiting the proliferation of hepatic stellate cells

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