中文｜English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Volume 37 Issue 6
Jun.  2021
Turn off MathJax
Article Contents
Article Navigation >  Journal of Clinical Hepatology  > 2021  >  37(6): 1379-1385

Effect of fecal microbiota transplantation on intestinal flora in mice with acute-on-chronic liver failure

DOI: 10.3969/j.issn.1001-5256.2021.06.031

  • Department of Infectious Diseases, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215000, China

  • Received Date: 2020-07-30
  • Accepted Date: 2020-08-31
  • Published Date: 2021-06-20
    Abstract
  •   Objective  To investigate the protective effect of fecal microbiota transplantation (FMT) on mice with acute-on-chronic liver failure (ACLF) and its effect on intestinal flora.  Methods  A total of 40 mice were randomly divided into control group (CON group), model group (MOD group), FMT group (feces of the mice in the CON group were used as fecal microbiota donor), and FMT model group (ANFMT group, with feces of the mice in the MOD group as fecal microbiota donor), with 10 mice in each group. All mice were observed in terms of body weight, death, liver histopathology, and changes in aspartate aminotransferase (AST), alanine aminotransferase (ALT), and intestinal flora. A one-way analysis of variance was used for comparison of normally distributed continuous data between multiple groups, and the SNK-q test was used for further comparison between two groups.  Results  Compared with the CON group, the MOD group had a significant reduction in body weight and significant increases in AST and ALT (all P < 0.05), as well as large patchy necrosis of hepatocytes, significant increases in Verrucomicrobia, Akkermansia, and Erysipelatoclostridium, and significant reductions in Dubosiella and Duncaniella (all P < 0.05). Compared with the CON group, the ANFMT group had a significant increase in AST (P < 0.05), hepatocyte swelling and mild ballooning degeneration, significant increases in Unclassified and Faecalibaculum, and significant reductions in Patescibacteria, Deferribacteres, Muribaculum, Candidatus_Saccharimonas, Rikenella, Odoribacter, Mucispirillum, and Lachnospiraceae_unclassified (all P < 0.05). Compared with the MOD group, the FMT group had significant reductions in AST and ALT (both P < 0.05), mild hepatocellular necrosis and marked ballooning degeneration, significant increases in Paramuribaculum and Bilophila, and significant reductions in Firmicutes, Rikenella, and Absiella (all P < 0.05).  Conclusion  Intestinal flora disturbance is observed in ACLF mice, and dysbacteriosis may lead to liver injury. FMT can alleviate liver inflammation in ACLF mice and thus exert a protective effect.

     

    • FullText(HTML)
  • loading
    • References(24)
  • [1]
    Liver Failure and Artificial Liver Group, Chinese Society of Infectious Diseases, Chinese Medical Association; Severe Liver Disease and Artificial Liver Group, Chinese Society of Hepatology, Chinese Medical Association. Guideline for diagnosis and treatment of liver failure(2018)[J]. J Clin Hepatol, 2019, 35(1): 38-44. DOI: 10.3969/j.issn.1001-5256.2019. 01.007.

    中华医学会感染病学分会肝衰竭与人工肝学组, 中华医学会肝病学分会重型肝病与人工肝学组. 肝衰竭诊治指南(2018年版)[J]. 临床肝胆病杂志, 2019, 35(1): 38-44. DOI: 10.3969/ j.issn.1001-5256.2019. 01.007.
    [2]
    CHEN Y, GUO J, QIAN G, et al. Gut dysbiosis in acute-on-chronic liver failure and its predictive value for mortality[J]. J Gastroenterol Hepatol, 2015, 30(9): 1429-1437. DOI: 10.1111/jgh.12932.
    [3]
    MINEMURA M, SHIMIZU Y. Gut microbiota and liver diseases[J]. World J Gastroenterol, 2015, 21(6): 1691-1702. DOI: 10.3748/wjg.v21.i6.1691.
    [4]
    CLEMENTE JC, URSELL LK, PARFREY LW, et al. The impact of the gut microbiota on human health: An integrative view[J]. Cell, 2012, 148(6): 1258-1270. DOI: 10.1016/j.cell.2012.01.035.
    [5]
    WANG KX, ZHENG C, ZHANG JH, et al. Clinical effect of Qingre Jiedu Liangxue prescription in treatment of mice with acute-on-chronic liver failure and related mechanism[J]. J Clin Hepatol, 2019, 35(12): 2765-2771. DOI: 10.3969/j.issn.1001-5256.2019.12.025.

    王开霞, 郑超, 张景豪, 等. 清热解毒凉血方治疗慢加急性肝衰竭小鼠模型的效果及机制[J]. 临床肝胆病杂志, 2019, 35(12): 2765-2771. DOI: 10.3969/j.issn.1001-5256.2019.12.025.
    [6]
    ZHANG YC, BI YZ, FANG X, et al. Protective effect of probiotics in rats with acute- on- chronic liver failure and related mechanism[J]. J Clin Hepatol, 2019, 35(7): 1570-1575. DOI: 10.3969/j.issn.1001-5256.2019.07.

    张永超, 毕研贞, 方萧, 等. 益生菌对慢加急性肝衰竭大鼠模型的保护作用及其机制[J]. 临床肝胆病杂志, 2019, 35(7): 1570-1575. DOI: 10.3969/j.issn.1001-5256.2019.07.
    [7]
    WANG WW, ZHANG Y, HUANG XB, et al. Fecal microbiota transplantation prevents hepatic encephalopathy in rats with carbon tetrachloride-induced acute hepatic dysfunction[J]. World J Gastroenterol, 2017, 23(38): 6983-6994. DOI: 10.3748/wjg.v23.i38.6983.
    [8]
    CUI B, FENG Q, WANG H, et al. Fecal microbiota transplantation through mid-gut for refractory Crohn's disease: Safety, feasibility, and efficacy trial results[J]. J Gastroenterol Hepatol, 2015, 30(1): 51-58. DOI: 10.1111/jgh.12727.
    [9]
    ROUND JL, MAZMANIAN SK. The gut microbiota shapes intestinal immune responses during health and disease[J]. Nat Rev Immunol, 2009, 9(5): 313-323. DOI: 10.1038/nri2515.
    [10]
    QIN N, YANG F, LI A, et al. Alterations of the human gut microbiome in liver cirrhosis[J]. Nature, 2014, 513(7516): 59-64. DOI: 10.1038/nature13568.
    [11]
    MOREAU R, JALAN R, GINES P, et al. Acute-on-chronic liver failure is a distinct syndrome that develops in patients with acute decompensation of cirrhosis[J]. Gastroenterology, 2013, 144(7): 1426-1437, 1437. e1-9. DOI: 10.1053/j.gastro.2013.02.042.
    [12]
    BAJAJ JS, FAGAN A, SIKAROODI M, et al. Liver transplant modulates gut microbial dysbiosis and cognitive function in cirrhosis[J]. Liver Transpl, 2017, 23(7): 907-914. DOI: 10.1002/lt.24754.
    [13]
    BAJAJ JS, IDILMAN R, MABUDIAN L, et al. Diet affects gut microbiota and modulates hospitalization risk differentially in an international cirrhosis cohort[J]. Hepatology, 2018, 68(1): 234-247. DOI: 10.1002/hep.29791.
    [14]
    ACHARYA C, BAJAJ JS. Gut microbiota and complications of liver disease[J]. Gastroenterol Clin North Am, 2017, 46(1): 155-169. DOI: 10.1016/j.gtc.2016.09.013.
    [15]
    DAVIS BC, BAJAJ JS. The human gut microbiome in liver diseases[J]. Semin Liver Dis, 2017, 37(2): 128-140. DOI: 10.1055/s-0037-1602763.
    [16]
    TILG H, CANI PD, MAYER EA. Gut microbiome and liver diseases[J]. Gut, 2016, 65(12): 2035-2044. DOI: 10.1136/gutjnl-2016-312729.
    [17]
    ZHANG W, GU Y, CHEN Y, et al. Intestinal flora imbalance results in altered bacterial translocation and liver function in rats with experimental cirrhosis[J]. Eur J Gastroenterol Hepatol, 2010, 22(12): 1481-1486. DOI: 10.1097/MEG.0b013e32833eb8b0.
    [18]
    PENG JH, CUI T, SUN ZL, et al. Effects of puerariae radix extract on endotoxin receptors and TNF-α expression induced by gut-derived endotoxin in chronic alcoholic liver injury[J]. Evid Based Complement Alternat Med, 2012, 2012: 234987. DOI: 10.1155/2012/234987.
    [19]
    BILZER M, ROGGEL F, GERBES AL. Role of Kupffer cells in host defense and liver disease[J]. Liver Int, 2006, 26(10): 1175-1186. DOI: 10.1111/j.1478-3231.2006.01342.x.
    [20]
    TAKEUCHI O, AKIRA S. Pattern recognition receptors and inflammation[J]. Cell, 2010, 140(6): 805-820. DOI: 10.1016/j.cell.2010.01.022.
    [21]
    XU L, KITADE H, NI Y, et al. Roles of chemokines and chemokine receptors in obesity-associated insulin resistance and nonalcoholic fatty liver disease[J]. Biomolecules, 2015, 5(3): 1563-1579. DOI: 10.3390/biom5031563.
    [22]
    WIEST R. Bacterial translocation and alterations of the digestive system[M]//GINÈS P, KAMATH P, ARROYO V. Chronic liver failure. Clinical Gastroenterology. Humana Press, 2011: 189-218.
    [23]
    MA HD, WANG YH, CHANG C, et al. The intestinal microbiota and microenvironment in liver[J]. Autoimmun Rev, 2015, 14(3): 183-191. DOI: 10.1016/j.autrev.2014.10.013.
    [24]
    YANG Y, JOBIN C. Microbial imbalance and intestinal pathologies: connections and contributions[J]. Dis Model Mech, 2014, 7(10): 1131-1142. DOI: 10.1242/dmm.016428.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(12)  / Tables(1)

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (1246) PDF downloads(68) Cited by()
    Proportional views
    Related
        

    The first journal specializing in hepatobiliary and pancreatic diseases in China

    Supervisor：Ministry of Education of the People's Republic of China

    Sponsor：Jilin University

    Academic Support: Chinese Society of Hepatology，Chinese Medical Association

    Address: 461 Xinjiang Road, Changchun

    Submit：0431-88782044

    Peer review：0431-88783542

    Email：lcgdb@vip.163.com

    About Journal

    Submission Guideline

    Journal Online

    Hepatobiliary College

    Guidelines

    YesterdayIP[]YesterdayPV[]TodayIP[]TodayPV[]Online[]

    Website Design © 2020 Editorial Board of Journal of Clinical Hepatology 吉ICP备10000617号-1 Supported by: Beijing Renhe Information Technology Co. Ltd  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return