Changes in the frequency, phenotype, and function of mucosal-associated invariant T lymphocytes in peripheral blood of children with metabolic associated fatty liver disease

Tao JIANG; Wenxian OUYANG; Yanfang TAN; Zhen KANG; Shuangjie LI

doi:10.3969/j.issn.1001-5256.2023.03.012

Volume 39 Issue 3

Mar. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Clinical Hepatology > 2023 > 39(3): 562-566

PDF( 1753 KB)

Changes in the frequency, phenotype, and function of mucosal-associated invariant T lymphocytes in peripheral blood of children with metabolic associated fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2023.03.012

Department of Hepatopathy Center, Hunan Children's Hospital, Changsha 410007, China

Research funding:

Scientific Research Project of Health and Family Planning Commission of Hunan Province (20200695)

More Information

Corresponding author: LI Shuangjie, lesjie62@vip.sina.com (ORCID: 0000-0002-3792-6793)
Received Date: 2022-10-21
Accepted Date: 2022-12-06
Published Date: 2023-03-20

Abstract

Abstract

Objective To investigate the change in mucosal-associated invariant T (MAIT) lymphocytes in peripheral blood of children with metabolic associated fatty liver disease (MAFLD) and its clinical significance. Methods A total of 18 children with MAFLD who attended Hunan Children's Hospital from March to May, 2022, were enrolled as MAFLD group, and 20 normal children who attended the hospital during the same period of time were enrolled as control group. Peripheral blood samples were collected, and flow cytometry was used to measure the percentages of MAIT lymphocytes (CD3⁺CDl61⁺TCRVα7.2⁺ cells), different MAIT lymphocyte subsets (CD4⁺CD8^- MAIT cells, CD4^-CD8^- MAIT lymphocytes, CD4^-CD8⁺ MAIT lymphocytes, and CD4⁺CD8⁺ MAIT lymphocytes), and MAIT lymphocytes expressing PD-1, CD69, perforin, CD107α, CXCR3, CXCR6, and CCR6. The correlation of MAIT lymphocyte frequency with liver inflammation, fat content, and fibrosis degree was analyzed. The t-test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups. The Spearman correlation analysis was used for correlation analysis. Results Compared with the control group, the MAFLD group had significant increases in the percentage of MAIT lymphocytes in peripheral blood, the percentages of MAIT cells expressing PD-1, CD69, CD107α, CXCR3, CXCR6 and CCR6, and the percentages of CD4⁺CD8^- MAIT cells and CD4⁺CD8⁺ MAIT lymphocytes among MAIT cell subsets (all P < 0.05), as well as a significant reduction in the percentage of CD4^-CD8⁺ MAIT cells among MAIT cell subsets (P < 0.001). The percentages of CD4⁺CD8⁺ MAIT lymphocytes and CD107α-positive MAIT lymphocytes were negatively correlated with alanine aminotransferase (r=-0.474 and -0.550, P=0.047 and 0.018). Conclusion The migration of peripheral blood MAIT lymphocytes to the liver plays a protective role against liver inflammation in children with MAFLD.
- Mucosal-Associated Invariant T Cells,
- Metabolic associated Fatty Liver Disease,
- Child

FullText(HTML)

References(19)

References

[1]	JIN R, WANG XX, LIU F, et al. Research advances in pharmacotherapy for nonalcoholic fatty liver disease[J]. J Clin Hepatol, 2022, 38(7): 1634-1640. DOI: 10.3969/j.issn.1001-5256.2022.07.033. 靳睿, 王晓晓, 刘峰, 等. 非酒精性脂肪性肝病的药物治疗进展[J]. 临床肝胆病杂志, 2022, 38(7): 1634-1640. DOI: 10.3969/j.issn.1001-5256.2022.07.033.
[2]	ESLAM M, ALKHOURI N, VAJRO P, et al. Defining paediatric metabolic (dysfunction)-associated fatty liver disease: an international expert consensus statement[J]. Lancet Gastroenterol Hepatol, 2021, 6(10): 864-873. DOI: 10.1016/S2468-1253(21)00183-7.
[3]	ESLAM M, NEWSOME PN, SARIN SK, et al. A new definition for metabolic dysfunction-associated fatty liver disease: An international expert consensus statement[J]. J Hepatol, 2020, 73(1): 202-209. DOI: 10.1016/j.jhep.2020.03.039.
[4]	FLISIAK-JACKIEWICZ M, LEBENSZTEJN DM. Update on pathogenesis, diagnostics and therapy of nonalcoholic fatty liver disease in children[J]. Clin Exp Hepatol, 2019, 5(1): 11-21. DOI: 10.5114/ceh.2019.83152.
[5]	CZAJA AJ. Incorporating mucosal-associated invariant T cells into the pathogenesis of chronic liver disease[J]. World J Gastroenterol, 2021, 27 (25): 3705-3733. DOI: 10.3748/wjg.v27.i25.3705.
[6]	LEGOUX F, BELLET D, DAVIAUD C, et al. Microbial metabolites control the thymic development of mucosal-associated invariant T cells[J]. Science, 2019, 366(6464): 494-499. DOI: 10.1126/science.aaw2719.
[7]	HEGDE P, WEISS E, PARADIS V, et al. Mucosal-associated invariant T cells are a profibrogenic immune cell population in the liver[J]. Nat Commun, 2018, 9(1): 2146. DOI: 10.1038/s41467-018-04450-y.
[8]	LI Y, HUANG B, JIANG X, et al. Mucosalassociated invariant T cells improve nonalcoholic fatty liver disease through regulating macrophage polarization[J]. Front Immunol, 2018, 9: 1994. DOI: 10.3389/fimmu.2018.01994.
[9]	DIEDRICH T, KUMMER S, GALANTE A, et al. Characterization of the immune cell landscape of patients with NAFLD[J]. PLoS One, 2020, 15(3): e0230307. DOI: 10.1371/journal.pone.0230307.
[10]	HE SL, LI SJ, LIU M, et al. Study on the diagnostic value of transient elastography, APRI and FIB-4 for liver fibrosis in children with non-alcoholic fatty liver disease[J]. Chin J Hepatol, 2022, 30(1): 81-86. DOI: 10.3760/cma.j.cn501113-20210105-00007. 何舒丽, 李双杰, 刘敏, 等. 瞬时弹性成像技术、APRI及FIB-4对儿童非酒精性脂肪性肝病肝纤维化诊断价值的研究[J]. 中华肝脏病杂志, 2022, 30(1): 81-86. DOI: 10.3760/cma.j.cn501113-20210105-00007.
[11]	LIU M, ZHENG X, QIU J, et al. Diagnostic value of CAP in children with nonalcoholic fatty liver disease[J]. J Clin Res, 2021, 38(3): 343-348. DOI: 10.3969/j.issn.1671-7171.2021.03.006. 刘敏, 郑曦, 仇君, 等. CAP对儿童非酒精性脂肪性肝病的诊断价值研究[J]. 医学临床研究, 2021, 38(3): 343-348. DOI: 10.3969/j.issn.1671-7171.2021.03.006.
[12]	CAROLAN E, TOBIN LM, MANGAN BA, et al. Altered distribution and increased IL-17 production by mucosal-associated invariant T cells in adult and childhood obesity[J]. J Immunol, 2015, 194(12): 5775-5780. DOI: 10.4049/jimmunol.1402945.
[13]	MAGALHAES I, PINGRIS K, POITOU C, et al. Mucosal-associated invariant T cell alterations in obese and type 2 diabetic patients[J]. J Clin Invest, 2015, 125(4): 1752-1762. DOI: 10.1172/JCI78941.
[14]	van HERCK MA, WEYLER J, KWANTEN WJ, et al. The differential roles of T cells in non-alcoholic fatty liver disease and obesity[J]. Front Immunol, 2019, 10: 82. DOI: 10.3389/fimmu.2019.00082.
[15]	LIU J, NAN H, BRUTKIEWICZ RR, et al. Sex discrepancy in the reduction of mucosal-associated invariant T cells caused by obesity[J]. Immun Inflamm Dis, 2021, 9(1): 299-309. DOI: 10.1002/iid3.393.
[16]	BERGIN R, KINLEN D, KEDIA-MEHTA N, et al. Mucosal-associated invariant T cells are associated with insulin resistance in childhood obesity, and disrupt insulin signalling via IL-17[J]. Diabetologia, 2022, 65(6): 1012-1017. DOI: 10.1007/s00125-022-05682-w.
[17]	NAIMIMOHASSES S, O'GORMAN P, WRIGHT C, et al. Differential effects of dietary versus exercise intervention on intrahepatic MAIT cells and histological features of NAFLD[J]. Nutrients, 2022, 14(11): 2198. DOI: 10.3390/nu14112198.
[18]	KOAY HF, GHERARDIN NA, ENDERS A, et al. A three-stage intrathymic development pathway for the mucosal-associated invariant T cell lineage[J]. Nat Immunol, 2016, 17(11): 1300-1311. DOI: 10.1038/ni.3565.
[19]	ZENG F, ZHANG Y, HAN X, et al. Predicting non-alcoholic fatty liver disease progression and immune deregulations by specific gene expression patterns[J]. Front Immunol, 2020, 11: 609900. DOI: 10.3389/fimmu.2020.609900.

Relative Articles

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Tables(4)

Get Citation

PDF

XML

Article Metrics

Article views (412) PDF downloads(66)

Changes in the frequency, phenotype, and function of mucosal-associated invariant T lymphocytes in peripheral blood of children with metabolic associated fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2023.03.012

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Changes in the frequency, phenotype, and function of mucosal-associated invariant T lymphocytes in peripheral blood of children with metabolic associated fatty liver disease

DOI: 10.3969/j.issn.1001-5256.2023.03.012

Abstract

References

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

About Journal

Submission Guideline

Journal Online

Hepatobiliary College

Guidelines

Export File

Citation

Format

Content